BBa_J119401 1 BBa_J119401 tCloneTet+PDB riboswitch with P5 and BD2 2016-07-17T11:00:00Z 2016-07-18T09:19:51Z J100255, original tClone tet+PDB Riboswitch, is located in Campbell M Lab. BD2, located in Eckdahl Lab, is part number J119025. The BD2 C Dog RBS has a lower binding efficiency than BD18. PCR Amplification was used to amplify the J100255 plasmid, excluding BD18, and BD2 was inserted via Decoupled Golden Gate Assembly. false false _613_ 606 606 61 false BD2 was inserted to the plasmid via Decoupled Golden Gate Assembly. Using iPCR, all of the J100255 plasmid was amplified, besides BD18. false Avery Umphreys annotation2481015 1 p5 range2481015 1 105 140 annotation2481016 1 TetA range2481016 1 310 1243 annotation2481014 1 BD2 range2481014 1 225 308 BBa_J119401_sequence 1 cccggaaaaacccaagcggattcatcgatgagctgacccgatcgccgccgccggagggttgcgtttgagacgggcgacagatgaattcgcggccgcttctagagttgacaattaatcatccggctcgtaatttatgtggacgacggcgataccagccgaaaggcccttggcagcgtctttacatactcggtaaacgacgctgccaaggttttttttgcggcgacgggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgctaaggaggttttctaatgggacttaatctaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagggcttgtttcggcgtgggtatggtggcaggccccgtggccggaggactgttgggcgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagagcgtcgaccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcactggtcccgcccaccaaacgttttcggcgaaaaagcagggccatttatcgcccggcatggccggcccgaaccccctggggctaccttcttggctgggctttccccaaccccgagggttgggatgggcctt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z