BBa_J119401
1
BBa_J119401
tCloneTet+PDB riboswitch with P5 and BD2
2016-07-17T11:00:00Z
2016-07-18T09:19:51Z
J100255, original tClone tet+PDB Riboswitch, is located in Campbell M Lab. BD2, located in Eckdahl Lab, is part number J119025.
The BD2 C Dog RBS has a lower binding efficiency than BD18. PCR Amplification was used to amplify the J100255 plasmid, excluding BD18, and BD2 was inserted via Decoupled Golden Gate Assembly.
false
false
_613_
606
606
61
false
BD2 was inserted to the plasmid via Decoupled Golden Gate Assembly. Using iPCR, all of the J100255 plasmid was amplified, besides BD18.
false
Avery Umphreys
annotation2481015
1
p5
range2481015
1
105
140
annotation2481016
1
TetA
range2481016
1
310
1243
annotation2481014
1
BD2
range2481014
1
225
308
BBa_J119401_sequence
1
cccggaaaaacccaagcggattcatcgatgagctgacccgatcgccgccgccggagggttgcgtttgagacgggcgacagatgaattcgcggccgcttctagagttgacaattaatcatccggctcgtaatttatgtggacgacggcgataccagccgaaaggcccttggcagcgtctttacatactcggtaaacgacgctgccaaggttttttttgcggcgacgggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgctaaggaggttttctaatgggacttaatctaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagggcttgtttcggcgtgggtatggtggcaggccccgtggccggaggactgttgggcgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagagcgtcgaccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcactggtcccgcccaccaaacgttttcggcgaaaaagcagggccatttatcgcccggcatggccggcccgaaccccctggggctaccttcttggctgggctttccccaaccccgagggttgggatgggcctt
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z