BBa_J119402 1 BBa_J119402 Double NiCo Riboswitch in tClone Red 2016-07-31T11:00:00Z 2016-08-01T01:50:06Z Synthetic oligonucleotides. A riboswitch found to be responsive to transitions metals (Ni++, Co++, Mn++) from Furakawa et al. (Molecular Cell 57: 1088-1098, 2015) was cloned as a tandem repeat into tClone Red using BsaI Golden Gate Assembly. false false _613_435_ 606 606 61 false No internal BsaI sites. false Anthony J. Eckdahl annotation2481371 1 BD18 C dog RBS range2481371 1 299 383 annotation2481372 1 RFP range2481372 1 384 1064 annotation2481368 1 P5 promoter range2481368 1 1 36 annotation2481369 1 NiCo Riboswitch range2481369 1 41 167 annotation2481370 1 NiCo Riboswitch range2481370 1 168 294 BBa_J119402_sequence 1 ttgacaattaatcatccggctcgtaatttatgtggacgacagtacaaactgagcaggcgatggacctttcatagaggtacatggggccgggccacccagtgagtggcagcagattgcaatcatgcacatctgtgggacagtagtatgttccacgggtgtgcttttttagtacaaactgagcaggcgatggacctttcatagaggtacatggggccgggccacccagtgagtggcagcagattgcaatcatgcacatctgtgggacagtagtatgttccacgggtgtgcttttttgcgggggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcgacggagcgtttctaatggcttcctccgaagatgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagatggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagatggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagattacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z