BBa_J119403 1 BBa_J119403 Triple NiCo Riboswitch in tClone Red 2016-07-31T11:00:00Z 2016-08-01T01:57:40Z Synthetic oligonucleotides. A riboswitch found to be responsive to transitions metals (Ni++, Co++, Mn++) from Furakawa et al. (Molecular Cell 57: 1088-1098, 2015) was cloned as three direct repeats into tClone Red using BsaI Golden Gate Assembly. false false _613_435_ 606 606 61 false No internal BsaI sites. false Anthony J. Eckdahl annotation2481378 1 RFP range2481378 1 511 1191 annotation2481374 1 NiCo Riboswitch range2481374 1 41 167 annotation2481375 1 NiCo Riboswitch range2481375 1 168 294 annotation2481377 1 NiCo Riboswitch range2481377 1 295 421 annotation2481376 1 BD18 C dog RBS range2481376 1 426 510 annotation2481379 1 P5 promoter range2481379 1 1 36 BBa_J119403_sequence 1 ttgacaattaatcatccggctcgtaatttatgtggacgacagtacaaactgagcaggcgatggacctttcatagaggtacatggggccgggccacccagtgagtggcagcagattgcaatcatgcacatctgtgggacagtagtatgttccacgggtgtgcttttttagtacaaactgagcaggcgatggacctttcatagaggtacatggggccgggccacccagtgagtggcagcagattgcaatcatgcacatctgtgggacagtagtatgttccacgggtgtgcttttttagtacaaactgagcaggcgatggacctttcatagaggtacatggggccgggccacccagtgagtggcagcagattgcaatcatgcacatctgtgggacagtagtatgttccacgggtgtgcttttttgcgggggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcgacggagcgtttctaatggcttcctccgaagatgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagatggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagatggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagattacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z