BBa_J120001 1 BBa_J120001 DFFb (CAD)-coding sequence for H. sapien DNA fragmentation factor beta 2012-04-09T11:00:00Z 2015-08-31T04:08:29Z NCBI accession #NM_004402.2 DFFb (DNA fragmentation factor beta polypeptide) a.k.a CAD (caspase activated DNase) is activated by cleavage from its inhibitor by Caspase 3. This is a key step in apoptosis. This sequence was originally obtained from NCBI (accession # NM_004402.2, but we modified the sequence to prevent cleavage by restriction enzymes at internal restriction sites. This was done by replacing the third base at each of these codons. false false _617_ 0 12905 9 Not in stock false There were two restriction enzyme recognition sites found within this coding sequence. The third base in codons at these sites were changed to avoid cleavage by restriction enzymes. false Corinthia Black, Sheree Harper, Srikanth Sistla, Anna Wieman BBa_J120001_sequence 1 atgctccagaagcccaagagcgtgaagctgcgggccctgcgcagcccgaggaagttcggcgtggctggccggagctgccaggaggtgctgcgcaagggctgtctccgcttccagctccctgagcgcggttcccggctgtgcctgtacgaggatggcacggagctgacggaagattacttccccagtgttcccgacaacgccgagctggtgctgctcaccttgggccaggcctggcagggctatgtgagcgacatcaggcgcttcctcagtgcatttcacgagccacaggtggggctcatccaggccgcccagcagctgctgtgtgatgagcaggccccacagaggcagaggctgctggctgacctcctgcacaacgtcagccagaacatcgcggccgagacccgggctgaggacccgccgtggtttgaaggcttggagtcccgatttcagagcaagtctggctatctgagatacagctgtgagagccggatccggagttacctgagggaggtgagctcctacccctccacggtgggtgcggaggctcaggaggagtttctgcgggtcctcggctccatgtgccagaggctccggtccatgcagtacaatggcagctacttcgacagaggagccaagggcggcagccgcctctgcacaccggaaggctggttctcctgccagggtccctttgacatggacagctgcttatcaagacactccatcaacccctacagtaacagggagagcaggatcctcttcagcacctggaacctggatcacataatagaaaagaaacgcaccatcattcctacactggtggaagcaattaaggaacaagatggaagagaagtggactgggagtatttttatggcctgctttttacctcagagaacctaaaactggtgcacattgtctgccataagaaaaccacccacaagctcaactgtgacccaagcagaatctacaaaccccagacaaggttgaagcggaagcagcctgtgcggaaacgccagtga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z