BBa_B0011
1
BBa_B0011
LuxICDABEG (+/-)
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
Released HQ 2013
Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.</p>
false
false
_1_
0
24
7
In stock
false
<P> <P>In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.<P>
true
Reshma Shetty
annotation7019
1
BBa_B0011
range7019
1
1
46
annotation1683
1
stem_loop
range1683
1
13
35
BBa_J120020
1
BBa_J120020
CH1 domain of human antibody
2012-04-02T11:00:00Z
2015-08-31T04:08:29Z
write later
write later
false
false
_617_
0
12298
9
Not in stock
false
write later
false
Xiaoyang Feng, Orijit Kar, Stephen Cain, Sanjana Gurrala, Sahil Sharma
BBa_K194001
1
BBa_K194001
GFP (a yeast- and FACS-optimized GFP variant),
2009-07-12T11:00:00Z
2015-05-08T01:11:16Z
Cormack BP, Bertram G, Egerton M, Gow NAR, Falkow S,
Brown AJP. 1997. Yeast-enhanced green ??uorescent protein
(yEGFP): a reporter of gene expression in Candida albicans.
Microbiology 143: 303??311.
Green Fluorescent Protein (GFP) codonoptimized for yeast.
false
false
_289_
0
3946
9
It's complicated
true
the biobrick follow the biobrick standards
false
Christian Kaas
annotation2011260
1
yeast GFP CDS
range2011260
1
1
713
BBa_J120027
1
BBa_J120027
It is a composite part containing CH1 domain of human antibody along with GFP.
2012-04-02T11:00:00Z
2015-08-31T04:08:29Z
genomic sequence.
The composite part contains constant region of the ADAM17 antibody (MO1) along with GFP (Part:BBa_K194001) whose expression will be observed in yeast. But for this device to work a promoter and a variable region is required to be present before the composite part. The variable region sequence is currently unknown and any promoter can be used which works in yeast.
false
false
_617_
0
12909
9
Not in stock
false
will be provided
false
Sanjana Gurrala, Xiaoyang Feng, Stephen Cain, Orijit Kar, Sahil Sharma
component2172065
1
BBa_K194001
component2172066
1
BBa_B0011
component2172063
1
BBa_J120020
annotation2172065
1
BBa_K194001
range2172065
1
298
1010
annotation2172063
1
BBa_J120020
range2172063
1
1
297
annotation2172066
1
BBa_B0011
range2172066
1
1011
1056
BBa_J120020_sequence
1
atggcaagcttcaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcaggcgccctgaccagcggcgtgcacaccttcccggctgtcctacagtcctcaggactctactccctcagcagcgtggtgaccgtgccctccagcagcttgggcacccagacctacatctgcaacgtgaatcacaagcccagcaacaccaaggtggacaagaaagtt
BBa_J120027_sequence
1
atggcaagcttcaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcaggcgccctgaccagcggcgtgcacaccttcccggctgtcctacagtcctcaggactctactccctcagcagcgtggtgaccgtgccctccagcagcttgggcacccagacctacatctgcaacgtgaatcacaagcccagcaacaccaaggtggacaagaaagttatgtctaaaggtgaagaattattcactggtgttgtcccaattttggttgaattagatggtgatgttaatggtcacaaattttctgtctccggtgaaggtgaaggtgatgctacttacggtaaattgaccttaaaatttatttgtactactggtaaattgccagttccatggccaaccttagtcactactttcggttatggtgttcaatgttttgcgagatacccagatcatatgaaacaacatgactttttcaagtctgccatgccagaaggttatgttcaagaaagaactatttttttcaaagatgacggtaactacaagaccagagctgaagtcaagtttgaaggtgataccttagttaatagaatcgaattaaaaggtattgattttaaagaagatggtaacattttaggtcacaaattggaatacaactataactctcacaatgtttacatcatggctgacaaacaaaagaatggtatcaaagttaacttcaaaattagacacaacattgaagatggttctgttcaattagctgaccattatcaacaaaatactccaattggtgatggtccagtcttgttaccagacaaccattacttatccactcaatctgccttatccaaagatccaaacgaaaagagagaccacatggtcttgttagaatttgttactgctgctggtattacccatggtatggatgaatttaataaagagaatataaaaagccagattattaatccggcttttttattattt
BBa_K194001_sequence
1
atgtctaaaggtgaagaattattcactggtgttgtcccaattttggttgaattagatggtgatgttaatggtcacaaattttctgtctccggtgaaggtgaaggtgatgctacttacggtaaattgaccttaaaatttatttgtactactggtaaattgccagttccatggccaaccttagtcactactttcggttatggtgttcaatgttttgcgagatacccagatcatatgaaacaacatgactttttcaagtctgccatgccagaaggttatgttcaagaaagaactatttttttcaaagatgacggtaactacaagaccagagctgaagtcaagtttgaaggtgataccttagttaatagaatcgaattaaaaggtattgattttaaagaagatggtaacattttaggtcacaaattggaatacaactataactctcacaatgtttacatcatggctgacaaacaaaagaatggtatcaaagttaacttcaaaattagacacaacattgaagatggttctgttcaattagctgaccattatcaacaaaatactccaattggtgatggtccagtcttgttaccagacaaccattacttatccactcaatctgccttatccaaagatccaaacgaaaagagagaccacatggtcttgttagaatttgttactgctgctggtattacccatggtatggatgaatttaataa
BBa_B0011_sequence
1
agagaatataaaaagccagattattaatccggcttttttattattt
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z