BBa_J120020 1 BBa_J120020 CH1 domain of human antibody 2012-04-02T11:00:00Z 2015-08-31T04:08:29Z write later write later false false _617_ 0 12298 9 Not in stock false write later false Xiaoyang Feng, Orijit Kar, Stephen Cain, Sanjana Gurrala, Sahil Sharma BBa_B0011 1 BBa_B0011 LuxICDABEG (+/-) 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>. Released HQ 2013 Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.</p> false false _1_ 0 24 7 In stock false <P> <P>In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A-&gt;G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.<P> true Reshma Shetty annotation7019 1 BBa_B0011 range7019 1 1 46 annotation1683 1 stem_loop range1683 1 13 35 BBa_I0500 1 pBad/araC Inducible pBad/araC promoter 2003-12-05T12:00:00Z 2015-08-31T04:07:29Z -- No description -- false true _1_ 0 24 7 In stock false true Sri Kosuri annotation1498842 1 PC range1498842 1 1043 1114 annotation1498843 1 AraC range1498843 1 1 1079 annotation1498841 1 Pbad range1498841 1 1080 1210 BBa_J120030 1 BBa_J120030 Composite part with Yeast promoter,CH1 domain of human Ab, ADAM17 cleavage sequence & Phoratoxin. 2012-04-03T11:00:00Z 2015-08-31T04:08:29Z later This device contains four sub-parts which will be produced in E.coli. These parts are E.coli promoter BBa_I0500, constant region of ADAM17 antibody which would be linked with cleavage site that is recognized by the ADAM17 proteolytic function knows as ???sheddase". Once cleaved, the phoratoxin which is linked to the other end of the cleavage site would be released and will lyse the cell membrane. This will be tested in the yeast cell in presence of another part BBa_J120026. false false _617_ 0 12298 9 Not in stock false Later false Xiaoyang Feng, Orijit Kar, Stephen Cain, Sanjana Gurrala, Sahil Sharma component2172078 1 BBa_J120023 component2172076 1 BBa_I0500 component2172079 1 BBa_J120022 component2172077 1 BBa_J120020 component2172080 1 BBa_B0011 annotation2172079 1 BBa_J120022 range2172079 1 1538 1678 annotation2172080 1 BBa_B0011 range2172080 1 1679 1724 annotation2172076 1 BBa_I0500 range2172076 1 1 1210 annotation2172077 1 BBa_J120020 range2172077 1 1211 1507 annotation2172078 1 BBa_J120023 range2172078 1 1508 1537 BBa_J120023 1 BBa_J120023 ADAM17 Cleavage Sequence 2012-04-02T11:00:00Z 2015-08-31T04:08:29Z to edit later Please see design considerations for a proper "long description" because at this time, we do not know how to make future edits to long descriptions. Thanks, and good day! false false _617_ 0 12907 9 Not in stock false to edit later false Orijit Kar, Xiaoyang Feng, Stephen Cain, Sanjana Gurrala, Sahil Sharma BBa_J120022 1 BBa_J120022 Phoratoxin 2012-04-02T11:00:00Z 2015-08-31T04:08:29Z Phoradendron tomentosum (California mistletoe) This is the 46 amino acid sequence for Phoratoxin, a thionin-type plant protein which are toxic to animal cells. They seem to exert their toxic effect at the level of the cell membrane, possibly through perforation of the membrane. Their precise function is not known. false false _617_ 0 12907 9 Not in stock false dunno at the moment... false Orijit Kar, Xiaoyang Feng, Stephen Cain, Sanjana Gurrala, Sahil Sharma BBa_J120022_sequence 1 aaaagctgctgcccgaccaccaccgcgcgcaacatttataacacctgccgctttggcggcggcagccgcccggtgtgcgcgaaactgagcggctgcaaaattattagcggcaccaaatgcgatagcggctggaaccattga BBa_J120020_sequence 1 atggcaagcttcaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcaggcgccctgaccagcggcgtgcacaccttcccggctgtcctacagtcctcaggactctactccctcagcagcgtggtgaccgtgccctccagcagcttgggcacccagacctacatctgcaacgtgaatcacaagcccagcaacaccaaggtggacaagaaagtt BBa_J120030_sequence 1 ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagcatggcaagcttcaagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcaggcgccctgaccagcggcgtgcacaccttcccggctgtcctacagtcctcaggactctactccctcagcagcgtggtgaccgtgccctccagcagcttgggcacccagacctacatctgcaacgtgaatcacaagcccagcaacaccaaggtggacaagaaagttccgctggcgcaggcggtgcgcagcagcagcaaaagctgctgcccgaccaccaccgcgcgcaacatttataacacctgccgctttggcggcggcagccgcccggtgtgcgcgaaactgagcggctgcaaaattattagcggcaccaaatgcgatagcggctggaaccattgaagagaatataaaaagccagattattaatccggcttttttattattt BBa_I0500_sequence 1 ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagc BBa_B0011_sequence 1 agagaatataaaaagccagattattaatccggcttttttattattt BBa_J120023_sequence 1 ccgctggcgcaggcggtgcgcagcagcagc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z