LOCUS       BBa_J125000              883 bp    DNA     linear       15-Nov-2024
DEFINITION  aaa
ACCESSION   BBa_J125000
VERSION     BBa_J125000.1  
FEATURES             Location/Qualifiers
     stem_loop       12..55
                     /label=stem_loop
     misc_feature    1..80
                     /label=BBa_B0010
     terminator      1..80
                     /label=BBa_B0010
     stem_loop       96..115
                     /label=T7 TE
     misc_feature    122..122
                     /label=stop
     polyA_site      116..129
                     /label=polya
     misc_feature    89..129
                     /label=BBa_B0012
     terminator      89..129
                     /label=BBa_B0012
     terminator      1..129
                     /label=BBa_B0015
     promoter        138..172
                     /label=BBa_J23100
     misc_feature    185..188
                     /label=conserved
     RBS             181..192
                     /label=BBa_B0034
     CDS             202..818
                     /label=tetR
     misc_feature    819..852
                     /label=SsrA
     misc_feature    859..883
                     /label=Help:Barcodes
     CDS             199..883
                     /label=BBa_C0040
ORIGIN
        1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
       61 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
      121 gcgtttatat actagagttg acggctagct cagtcctagg tacagtgcta gctactagag
      181 aaagaggaga aatactagat gtccagatta gataaaagta aagtgattaa cagcgcatta
      241 gagctgctta atgaggtcgg aatcgaaggt ttaacaaccc gtaaactcgc ccagaagcta
      301 ggtgtagagc agcctacatt gtattggcat gtaaaaaata agcgggcttt gctcgacgcc
      361 ttagccattg agatgttaga taggcaccat actcactttt gccctttaga aggggaaagc
      421 tggcaagatt ttttacgtaa taacgctaaa agttttagat gtgctttact aagtcatcgc
      481 gatggagcaa aagtacattt aggtacacgg cctacagaaa aacagtatga aactctcgaa
      541 aatcaattag cctttttatg ccaacaaggt ttttcactag agaatgcatt atatgcactc
      601 agcgctgtgg ggcattttac tttaggttgc gtattggaag atcaagagca tcaagtcgct
      661 aaagaagaaa gggaaacacc tactactgat agtatgccgc cattattacg acaagctatc
      721 gaattatttg atcaccaagg tgcagagcca gccttcttat tcggccttga attgatcata
      781 tgcggattag aaaaacaact taaatgtgaa agtgggtccg ctgcaaacga cgaaaactac
      841 gctttagtag cttaataaca ctgatagtgc tagtgtagat cac
//