LOCUS       BBa_J13000               571 bp    DNA     linear       14-May-2024
DEFINITION  3OC6HSL+LuxR dependent LacZalpha generator
ACCESSION   BBa_J13000
VERSION     BBa_J13000.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=LuxR/HSL
     promoter        20..25
                     /label=-35
     promoter        42..47
                     /label=-10
     misc_feature    53..53
                     /label=start
     misc_feature    1..55
                     /label=BBa_R0062
     promoter        1..55
                     /label=BBa_R0062
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     CDS             82..429
                     /label=lacZ alpha
     misc_feature    162..162
                     /label=T
     misc_feature    168..168
                     /label=A
     misc_feature    189..189
                     /label=C
     misc_feature    159..159
                     /label=C
     misc_feature    192..192
                     /label=G
     promoter        107..116
                     /label=T3 promoter
     promoter        254..272
                     /label=T7 promoter
     misc_feature    280..429
                     /label=lacZ gene fragment
     misc_feature    82..96
                     /label=lacZ gene fragment
     misc_feature    82..434
                     /label=BBa_E0033
     stem_loop       454..497
                     /label=stem_loop
     misc_feature    443..522
                     /label=BBa_B0010
     terminator      443..522
                     /label=BBa_B0010
     stem_loop       538..557
                     /label=T7 TE
     misc_feature    564..564
                     /label=stop
     polyA_site      558..571
                     /label=polya
     misc_feature    531..571
                     /label=BBa_B0012
     terminator      531..571
                     /label=BBa_B0012
     terminator      443..571
                     /label=BBa_B0015
     misc_feature    64..571
                     /label=BBa_E0433
ORIGIN
        1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta
       61 gagaaagagg agaaatacta gatgaccatg attacgccaa gcgcgcaatt aaccctcact
      121 aaagggaaca aaagctggag ctccaccgcg gtggcggcag cactagagct agtggatccc
      181 ccgggctgta gaaattcgat atcaagctta tcgataccgt cgacctcgag ggggggcccg
      241 gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa
      301 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
      361 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
      421 agcctgaata ataatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac
      481 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc
      541 tcaccttcgg gtgggccttt ctgcgtttat a
//