##gff-version 3
##sequence-region BBa_J13001 1 2605
BBa_J13001	.	CDS	820	1437	.	+	0	ID=BBa_C0061;Name=BBa_C0061
BBa_J13001	.	engineered_tag	1438	1462	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_J13001	.	tag	1399	1430	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_J13001	.	engineered_region	820	1437	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_J13001	.	CDS	820	1398	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_J13001	.	ribosome_entry_site	802	813	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J13001	.	conserved_region	806	809	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J13001	.	terminator	1471	1550	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J13001	.	stem_loop	1482	1525	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J13001	.	engineered_region	1471	1550	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J13001	.	terminator	2477	2556	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J13001	.	stem_loop	2488	2531	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J13001	.	engineered_region	2477	2556	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J13001	.	ribosome_entry_site	1670	1681	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J13001	.	conserved_region	1674	1677	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J13001	.	terminator	1559	1599	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J13001	.	stem_loop	1566	1585	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J13001	.	stop_codon	1592	1592	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J13001	.	engineered_region	1559	1599	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J13001	.	polyA_site	1586	1599	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J13001	.	CDS	1688	2443	.	+	0	ID=BBa_C0062;Name=BBa_C0062
BBa_J13001	.	CDS	1688	2437	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_J13001	.	engineered_region	1688	2443	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_J13001	.	engineered_region	1688	1689	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_J13001	.	point_mutation	1861	1861	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_J13001	.	engineered_tag	2444	2468	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_J13001	.	point_mutation	2179	2179	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_J13001	.	terminator	2565	2605	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J13001	.	stem_loop	2572	2591	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J13001	.	stop_codon	2598	2598	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J13001	.	engineered_region	2565	2605	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J13001	.	polyA_site	2592	2605	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J13001	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J13001	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J13001	.	promoter	1608	1661	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_J13001	.	non_covalent_binding_site	1608	1626	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J13001	.	engineered_region	1608	1661	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J13001	.	promoter	1627	1632	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J13001	.	promoter	1650	1655	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J13001	.	non_covalent_binding_site	1633	1651	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J13001	.	CDS	19	768	.	+	0	ID=BBa_C0051;Name=BBa_C0051
BBa_J13001	.	tag	730	762	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_J13001	.	engineered_tag	769	793	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_J13001	.	CDS	22	729	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
>BBa_J13001
aaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaag
caatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcagg
cgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagc
gttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaa
gtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtga
tgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgacc
gcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtg
atttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttaca
accactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtgg
cctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagat
cgctactagagaaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggagga
gtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataacctt
gaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggc
gtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaaga
tcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattaca
atgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaa
tagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaa
atcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgct
ttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtc
gaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggt
gggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagc
actactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaa
gcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcg
cgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatatta
tgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatata
tttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggttta
gtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatag
tttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagca
aataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttggg
atatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaac
aaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactga
tagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttt
tatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata