LOCUS BBa_J13007 2765 bp DNA linear 25-Nov-2024 DEFINITION Polycistronic CFP/YFP + Monocistronic mCherry ACCESSION BBa_J13007 VERSION BBa_J13007.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 CDS 81..803 /label=BBa_E0020 misc_feature 816..819 /label=conserved RBS 812..823 /label=BBa_B0034 CDS 830..1552 /label=BBa_E0030 stem_loop 1572..1615 /label=stem_loop misc_feature 1561..1640 /label=BBa_B0010 terminator 1561..1640 /label=BBa_B0010 stem_loop 1656..1675 /label=T7 TE misc_feature 1682..1682 /label=stop polyA_site 1676..1689 /label=polya misc_feature 1649..1689 /label=BBa_B0012 terminator 1649..1689 /label=BBa_B0012 stem_loop 1709..1752 /label=stem_loop misc_feature 1698..1777 /label=BBa_B0010 terminator 1698..1777 /label=BBa_B0010 stem_loop 1793..1812 /label=T7 TE misc_feature 1819..1819 /label=stop polyA_site 1813..1826 /label=polya misc_feature 1786..1826 /label=BBa_B0012 terminator 1786..1826 /label=BBa_B0012 misc_feature 1835..1853 /label=TetR 1 misc_feature 1835..1888 /label=BBa_R0040 promoter 1854..1859 /label=-35 misc_feature 1860..1878 /label=TetR 2 promoter 1877..1882 /label=-10 promoter 1835..1888 /label=BBa_R0040 misc_feature 1901..1904 /label=conserved RBS 1897..1908 /label=BBa_B0034 CDS 1915..2625 /label=mCherry misc_feature 2266..2266 /label=C->T (removing PstI site) misc_feature 1915..2628 /label=BBa_J06504 stem_loop 2648..2691 /label=stem_loop misc_feature 2637..2716 /label=BBa_B0010 terminator 2637..2716 /label=BBa_B0010 stem_loop 2732..2751 /label=T7 TE misc_feature 2758..2758 /label=stop polyA_site 2752..2765 /label=polya misc_feature 2725..2765 /label=BBa_B0012 terminator 2725..2765 /label=BBa_B0012 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atggtgagca agggcgagga gctgttcacc ggggtggtgc 121 ccatcctggt cgagctggac ggcgacgtga acggccacaa gttcagcgtg tccggcgagg 181 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc 241 tgcccgtgcc ctggcccacc ctcgtgacca ccctgacctg gggcgtgcag tgcttcagcc 301 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg 361 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga 421 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg 481 acggcaacat cctggggcac aagctggagt acaactacat cagccacaac gtctatatca 541 ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa gatccgccac aacatcgagg 601 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg 661 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg 721 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca 781 tggacgagct gtacaagtaa taatactaga gaaagaggag aaatactaga tggtgagcaa 841 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa 901 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac 961 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac 1021 cttcggctac ggcctgcaat gcttcgcccg ctaccccgac cacatgaagc tgcacgactt 1081 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga 1141 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat 1201 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta 1261 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt 1321 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca 1381 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcta 1441 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt 1501 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaat aatactagag 1561 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt 1621 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct 1681 gcgtttatat actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc 1741 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc 1801 ttcgggtggg cctttctgcg tttatatact agagtcccta tcagtgatag agattgacat 1861 ccctatcagt gatagagata ctgagcacta ctagagaaag aggagaaata ctagatggtg 1921 agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac 1981 atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc 2041 tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc 2101 tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc 2161 gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg 2221 aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccttgca ggacggcgag 2281 ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag 2341 aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg 2401 aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc 2461 aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc 2521 aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc 2581 gagggccgcc actccaccgg cggcatggac gagctgtaca agtaataata ctagagccag 2641 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt 2701 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt 2761 ttata //