BBa_J13007
type: ComponentDefinition
persistent identity: BBa J13007
display id: BBa_J13007
version: 1
was generated by: iGEM to SBOL conversion
description: Polycistronic CFP/YFP + Monocistronic mCherry
created: 2006-06-21T11:00:00Z
modified: 2015-08-31T04:08:29Z
mutable provenance: Registry
owned by: james
owned by: myers
mutable description: Polycistronic CFP/YFP + Monocistronic mCherry both driven by R0040 and all with the RBS B0034
discontinued: false
dominant: false
experience: experience/None
group u list: _5_
m user id: 0
owner id: 88
owning group id: 85
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None
star: false
creator: Jeffrey J Tabor
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_E0020
component: BBa_B0012
component: BBa_B0010
component: BBa_J06504
component: BBa_R0040
component: BBa_E0030
sequence annotation: BBa_R0040
sequence annotation: BBa_B0034
sequence annotation: BBa_E0020
sequence annotation: BBa_E0030
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_J06504
sequence: https://synbiohub.org/public/igem/BBa_J13007_sequence/1

BBa_B0034
type: Component
persistent identity: component1881966
display id: component1881966
version: 1
top level: BBa_J13007
definition: BBa_B0034
access: public

BBa_E0020
type: Component
persistent identity: component1881964
display id: component1881964
version: 1
top level: BBa_J13007
definition: ecfp
access: public

BBa_B0012
type: Component
persistent identity: component1881992
display id: component1881992
version: 1
top level: BBa_J13007
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1881990
display id: component1881990
version: 1
top level: BBa_J13007
definition: BBa_B0010
access: public

BBa_J06504
type: Component
persistent identity: component1881989
display id: component1881989
version: 1
top level: BBa_J13007
definition: mCherry
access: public

BBa_B0034
type: Component
persistent identity: component1881986
display id: component1881986
version: 1
top level: BBa_J13007
definition: BBa_B0034
access: public

BBa_B0034
type: Component
persistent identity: component1881963
display id: component1881963
version: 1
top level: BBa_J13007
definition: BBa_B0034
access: public

BBa_R0040
type: Component
persistent identity: component1881958
display id: component1881958
version: 1
top level: BBa_J13007
definition: p(tetR)
access: public

BBa_R0040
type: Component
persistent identity: component1881981
display id: component1881981
version: 1
top level: BBa_J13007
definition: p(tetR)
access: public

BBa_B0012
type: Component
persistent identity: component1881976
display id: component1881976
version: 1
top level: BBa_J13007
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1881974
display id: component1881974
version: 1
top level: BBa_J13007
definition: BBa_B0010
access: public

BBa_B0012
type: Component
persistent identity: component1881970
display id: component1881970
version: 1
top level: BBa_J13007
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1881968
display id: component1881968
version: 1
top level: BBa_J13007
definition: BBa_B0010
access: public

BBa_E0030
type: Component
persistent identity: component1881967
display id: component1881967
version: 1
top level: BBa_J13007
definition: eyfp
access: public

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1881958
display id: annotation1881958
version: 1
top level: BBa_J13007
location: https://synbiohub.org/public/igem/BBa_J13007/annotation1881958/range1881958/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_J13007/annotation1881958/range1881958/1
type: Range
persistent identity: range1881958
display id: range1881958
version: 1
top level: BBa_J13007
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1881963
display id: annotation1881963
version: 1
top level: BBa_J13007
location: https://synbiohub.org/public/igem/BBa_J13007/annotation1881963/range1881963/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J13007/annotation1881963/range1881963/1
type: Range
persistent identity: range1881963
display id: range1881963
version: 1
top level: BBa_J13007
direction: direction/reverse
start: 63
end: 74
orientation: inline

BBa_E0020
type: SequenceAnnotation
persistent identity: annotation1881964
display id: annotation1881964
version: 1
top level: BBa_J13007
location: https://synbiohub.org/public/igem/BBa_J13007/annotation1881964/range1881964/1
component: BBa_E0020

https://synbiohub.org/public/igem/BBa_J13007/annotation1881964/range1881964/1
type: Range
persistent identity: range1881964
display id: range1881964
version: 1
top level: BBa_J13007
direction: direction/reverse
start: 81
end: 803
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1881966
display id: annotation1881966
version: 1
top level: BBa_J13007
location: https://synbiohub.org/public/igem/BBa_J13007/annotation1881966/range1881966/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J13007/annotation1881966/range1881966/1
type: Range
persistent identity: range1881966
display id: range1881966
version: 1
top level: BBa_J13007
direction: direction/reverse
start: 812
end: 823
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation1881967
display id: annotation1881967
version: 1
top level: BBa_J13007
location: https://synbiohub.org/public/igem/BBa_J13007/annotation1881967/range1881967/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_J13007/annotation1881967/range1881967/1
type: Range
persistent identity: range1881967
display id: range1881967
version: 1
top level: BBa_J13007
direction: direction/reverse
start: 830
end: 1552
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1881968
display id: annotation1881968
version: 1
top level: BBa_J13007
location: https://synbiohub.org/public/igem/BBa_J13007/annotation1881968/range1881968/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J13007/annotation1881968/range1881968/1
type: Range
persistent identity: range1881968
display id: range1881968
version: 1
top level: BBa_J13007
direction: direction/forward
start: 1561
end: 1640
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1881970
display id: annotation1881970
version: 1
top level: BBa_J13007
location: https://synbiohub.org/public/igem/BBa_J13007/annotation1881970/range1881970/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J13007/annotation1881970/range1881970/1
type: Range
persistent identity: range1881970
display id: range1881970
version: 1
top level: BBa_J13007
direction: direction/forward
start: 1649
end: 1689
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1881974
display id: annotation1881974
version: 1
top level: BBa_J13007
location: https://synbiohub.org/public/igem/BBa_J13007/annotation1881974/range1881974/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J13007/annotation1881974/range1881974/1
type: Range
persistent identity: range1881974
display id: range1881974
version: 1
top level: BBa_J13007
direction: direction/forward
start: 1698
end: 1777
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1881976
display id: annotation1881976
version: 1
top level: BBa_J13007
location: https://synbiohub.org/public/igem/BBa_J13007/annotation1881976/range1881976/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J13007/annotation1881976/range1881976/1
type: Range
persistent identity: range1881976
display id: range1881976
version: 1
top level: BBa_J13007
direction: direction/forward
start: 1786
end: 1826
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1881981
display id: annotation1881981
version: 1
top level: BBa_J13007
location: https://synbiohub.org/public/igem/BBa_J13007/annotation1881981/range1881981/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_J13007/annotation1881981/range1881981/1
type: Range
persistent identity: range1881981
display id: range1881981
version: 1
top level: BBa_J13007
direction: direction/forward
start: 1835
end: 1888
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1881986
display id: annotation1881986
version: 1
top level: BBa_J13007
location: https://synbiohub.org/public/igem/BBa_J13007/annotation1881986/range1881986/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J13007/annotation1881986/range1881986/1
type: Range
persistent identity: range1881986
display id: range1881986
version: 1
top level: BBa_J13007
direction: direction/reverse
start: 1897
end: 1908
orientation: inline

BBa_J06504
type: SequenceAnnotation
persistent identity: annotation1881989
display id: annotation1881989
version: 1
top level: BBa_J13007
location: https://synbiohub.org/public/igem/BBa_J13007/annotation1881989/range1881989/1
component: BBa_J06504

https://synbiohub.org/public/igem/BBa_J13007/annotation1881989/range1881989/1
type: Range
persistent identity: range1881989
display id: range1881989
version: 1
top level: BBa_J13007
direction: direction/reverse
start: 1915
end: 2628
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1881990
display id: annotation1881990
version: 1
top level: BBa_J13007
location: https://synbiohub.org/public/igem/BBa_J13007/annotation1881990/range1881990/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J13007/annotation1881990/range1881990/1
type: Range
persistent identity: range1881990
display id: range1881990
version: 1
top level: BBa_J13007
direction: direction/forward
start: 2637
end: 2716
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1881992
display id: annotation1881992
version: 1
top level: BBa_J13007
location: https://synbiohub.org/public/igem/BBa_J13007/annotation1881992/range1881992/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J13007/annotation1881992/range1881992/1
type: Range
persistent identity: range1881992
display id: range1881992
version: 1
top level: BBa_J13007
direction: direction/forward
start: 2725
end: 2765
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

eyfp
type: ComponentDefinition
persistent identity: BBa E0030
display id: BBa_E0030
version: 1
was generated by: iGEM to SBOL conversion
description: enhanced yellow fluorescent protein derived from A. victoria GFP
created: 2004-03-02T12:00:00Z
modified: 2015-08-31T04:07:25Z
mutable provenance: Modificaitons to Clontech EYFP by Reshma Shetty
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Caitlin Conboy and Jennifer Braff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_E0030_sequence/1

ecfp
type: ComponentDefinition
persistent identity: BBa E0020
display id: BBa_E0020
version: 1
was generated by: iGEM to SBOL conversion
description: engineered cyan fluorescent protein derived from A. victoria GFP
created: 2004-03-02T12:00:00Z
modified: 2015-08-31T04:07:25Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Caitlin Conboy and Jennifer Braff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_E0020_sequence/1

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

mCherry
type: ComponentDefinition
persistent identity: BBa J06504
display id: BBa_J06504
version: 1
was generated by: iGEM to SBOL conversion
description: monomeric RFP optimized for bacteria
created: 2005-07-17T11:00:00Z
modified: 2016-01-25T01:05:28Z
mutable provenance: mCherry from Roger Y. Tsien's lab, altered to be BioBrick compatible
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: mRFP (DsRed) derived, altered to be a biobrick by removing a PstI site and adding in the ends.
discontinued: false
dominant: false
experience: experience/None
group u list: _20_
m user id: 4206
owner id: 340
owning group id: 20
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Made a point mutation to eliminate a PstI site in the middle and then added BioBrick ends using PCR. Sequenced using primers that bind to the pSB1A2 plasmid on either side of the brick.
star: true
creator: ytwang
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: mCherry
sequence annotation: C->T (removing PstI site)
sequence: https://synbiohub.org/public/igem/BBa_J06504_sequence/1

mCherry
type: SequenceAnnotation
persistent identity: annotation1585829
display id: annotation1585829
version: 1
top level: mCherry
location: https://synbiohub.org/public/igem/BBa_J06504/annotation1585829/range1585829/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_J06504/annotation1585829/range1585829/1
type: Range
persistent identity: range1585829
display id: range1585829
version: 1
top level: mCherry
direction: direction/forward
start: 1
end: 711
orientation: inline

C->T (removing PstI site)
type: SequenceAnnotation
persistent identity: annotation1585833
display id: annotation1585833
version: 1
top level: mCherry
location: https://synbiohub.org/public/igem/BBa_J06504/annotation1585833/range1585833/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_J06504/annotation1585833/range1585833/1
type: Range
persistent identity: range1585833
display id: range1585833
version: 1
top level: mCherry
direction: direction/forward
start: 352
end: 352
orientation: inline

https://synbiohub.org/public/igem/BBa_J13007_sequence/1
type: Sequence
persistent identity: BBa J13007 sequence
display id: BBa_J13007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataatactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacgg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0030_sequence/1
type: Sequence
persistent identity: BBa E0030 sequence
display id: BBa_E0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0020_sequence/1
type: Sequence
persistent identity: BBa E0020 sequence
display id: BBa_E0020_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J06504_sequence/1
type: Sequence
persistent identity: BBa J06504 sequence
display id: BBa_J06504_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z