LOCUS       BBa_J13008               969 bp    DNA     linear       16-Feb-2025
DEFINITION  LacI repressed ho1 from synechosytsis
ACCESSION   BBa_J13008
VERSION     BBa_J13008.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     misc_feature    808..832
                     /label=Help:Barcodes
     CDS             82..807
                     /label=BBa_I15008
     stem_loop       852..895
                     /label=stem_loop
     misc_feature    841..920
                     /label=BBa_B0010
     terminator      841..920
                     /label=BBa_B0010
     stem_loop       936..955
                     /label=T7 TE
     misc_feature    962..962
                     /label=stop
     polyA_site      956..969
                     /label=polya
     misc_feature    929..969
                     /label=BBa_B0012
     terminator      929..969
                     /label=BBa_B0012
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatgagtgtc aacttagctt cccagttgcg ggaagggacg
      121 aaaaaatccc actccatggc ggagaacgtc ggctttgtca aatgcttcct caagggcgtt
      181 gtcgagaaaa attcctaccg taagctggtt ggcaatctct actttgtcta cagtgccatg
      241 gaagaggaaa tggcaaaatt taaggaccat cccatcctca gccacattta cttccccgaa
      301 ctcaaccgca aacaaagcct agagcaagac ctgcaattct attacggctc caactggcgg
      361 caagaagtga aaatttctgc cgctggccaa gcctatgtgg accgagtccg gcaagtggcc
      421 gctacggccc ctgaattgtt ggtggcccat tcctacaccc gttacctggg ggatctttcc
      481 ggcggtcaaa ttctcaagaa aattgcccaa aatgccatga atctccacga tggtggcaca
      541 gctttctatg aatttgccga cattgatgac gaaaaggctt ttaaaaatac ctaccgtcaa
      601 gctatgaatg atctgcccat tgaccaagcc accgccgaac ggattgtgga tgaagccaat
      661 gacgcctttg ccatgaacat gaaaatgttc aacgaacttg aaggcaacct gatcaaggcg
      721 atcggcatta tggtgttcaa cagcctcacc cgtcgccgca gtcaaggcag caccgaagtt
      781 ggcctcgcca cctccgaagg ctaataacgc tgatagtgct agtgtagatc gctactagag
      841 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
      901 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
      961 gcgtttata
//