LOCUS       BBa_J13011              2480 bp    DNA     linear       20-Nov-2024
DEFINITION  TetR repressed Cph8
ACCESSION   BBa_J13011
VERSION     BBa_J13011.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..2318
                     /label=Cph8
     misc_feature    2319..2343
                     /label=Help:Barcodes
     CDS             81..2318
                     /label=BBa_I15010
     stem_loop       2363..2406
                     /label=stem_loop
     misc_feature    2352..2431
                     /label=BBa_B0010
     terminator      2352..2431
                     /label=BBa_B0010
     stem_loop       2447..2466
                     /label=T7 TE
     misc_feature    2473..2473
                     /label=stop
     polyA_site      2467..2480
                     /label=polya
     misc_feature    2440..2480
                     /label=BBa_B0012
     terminator      2440..2480
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atggccacca ccgtacaact cagcgaccaa tccctccgtc
      121 agctagaaac cctcgccatc cacaccgccc acctgattca gccccacggt ttagtggtgg
      181 tcctgcaaga accagacctc accatcagcc aaattagcgc caactgcacc ggcattttag
      241 ggcgatcgcc agaggatttg ttgggcagaa ccctagggga agtgtttgat agctttcaga
      301 ttgatcccat ccagagtcgc ctaacggccg gacaaatcag cagcctcaac cccagtaaac
      361 tttgggcgcg ggtcatgggg gacgactttg tcatttttga cggggttttt catcgcaaca
      421 gtgacggttt attggtatgt gaactcgagc cagcctacac ttccgataat ctgcccttcc
      481 tcggttttta tcacatggcc aacgctgccc tgaatcggtt gcgccaacaa gctaatctac
      541 gggatttcta cgatgttatt gtcgaagaag tccgccgtat gactggcttt gaccgggtga
      601 tgctataccg ctttgatgaa aataaccacg gtgatgtcat tgccgaagat aaacgggatg
      661 atatggaacc ctatttgggc ctgcactatc ccgaatcgga tattccccaa cccgcccgtc
      721 ggctatttat ccacaacccc attcgagtaa ttcccgatgt ttatggtgtg gcggtgcccc
      781 tgaccccagc ggttaacccc agcaccaacc gagcggtgga tttaacagaa tccattctgc
      841 gcagtgcgta ccattgccac ttgacctatc tgaaaaatat gggggtagga gcgtctttaa
      901 ccatttccct aattaaggac ggccatctct gggggctcat tgcctgccac catcaaaccc
      961 ccaaagtaat tccctttgaa ctgcgtaaag cctgcgaatt ttttggtcgg gtggtgttta
     1021 gcaacatttc cgcccaggaa gatacggaaa ccttcgatta ccgggtgcag ctggcggagc
     1081 atgaagcggt tttattggac aaaatgacca cggcggcgga ttttgtcgaa ggattaacta
     1141 atcatcccga tcgcctgttg ggattaacgg gctcccaggg ggcggccatt tgctttgggg
     1201 aaaaattgat tttagtaggg gaaaccccgg acgagaaagc agtgcaatat ttactgcaat
     1261 ggttggagaa tcgggaagtg caagacgttt tcttcacctc ttccctctca caaatttatc
     1321 ctgatgcagt gaattttaaa tccgtggcca gtggcttatt ggccattccc attgcccgtc
     1381 acaacttttt gctctggttt cgccctgaag tgttgcaaac ggttaattgg ggcggtgacc
     1441 caaatcatgc ttacgaagct acccaggaag acggtaaaat cgagctccat ccccgccaat
     1501 cctttgacct ctggaaagaa attgtccgac tccaatcttt gccctggcaa tcggtggaaa
     1561 tccaaagtgc cctggccctg aaaaaggcga tcgtcaacct cattttgcgc caggcagaag
     1621 aattgcatat ggcggctggt gttaagcaac tggcggatga ccgcacgctg ctgatggcgg
     1681 gggtaagtca cgacttgcgc acgccgctga cgcgtattcg cctggcgact gagatgatga
     1741 gcgagcagga tggctatctg gcagaatcga tcaataaaga tatcgaagag tgcaacgcca
     1801 tcattgagca gtttatcgac tacctgcgca ccgggcagga gatgccgatg gaaatggcgg
     1861 atcttaatgc agtactcggt gaggtgattg ctgccgaaag tggctatgag cgggaaattg
     1921 aaaccgcgct ttaccccggc agcattgaag tgaaaatgca cccgctgtcg atcaaacgcg
     1981 cggtggcgaa tatggtggtc aacgccgccc gttatggcaa tggctgggtc aaagtcagca
     2041 gcggaacgga gccgaatcgc gcctggttcc aggtggaaga tgacggtccg ggaattgcgc
     2101 cggaacaacg taagcacctg ttccagccgt ttgtccgcgg cgacagtgcg cgcaccatta
     2161 gcggcacggg attagggctg gcaattgtgc agcgtatcgt ggataaccat aacgggatgc
     2221 tggagcttgg caccagcgag cggggcgggc tttccattcg cgcctggctg ccagtgccgg
     2281 taacgcgggc gcagggcatg acaaaagaag ggtaataact ctgatagtgc tagtgtagat
     2341 ctctactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     2401 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg
     2461 tgggcctttc tgcgtttata
//