LOCUS       BBa_J13012               992 bp    DNA     linear       20-Nov-2024
DEFINITION  Tet repressible ho1
ACCESSION   BBa_J13012
VERSION     BBa_J13012.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..830
                     /label=PcyA
     misc_feature    831..855
                     /label=Help:Barcodes
     CDS             81..830
                     /label=BBa_I15009
     stem_loop       875..918
                     /label=stem_loop
     misc_feature    864..943
                     /label=BBa_B0010
     terminator      864..943
                     /label=BBa_B0010
     stem_loop       959..978
                     /label=T7 TE
     misc_feature    985..985
                     /label=stop
     polyA_site      979..992
                     /label=polya
     misc_feature    952..992
                     /label=BBa_B0012
     terminator      952..992
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atggccgtca ctgatttaag tttgaccaat tcttccctga
      121 tgcctacgtt gaacccgatg attcaacagt tggccctggc gatcgccgct agttggcaaa
      181 gtttacccct caagccctat caattgccgg aggatttggg ctacgtagaa ggccgcctgg
      241 aaggggaaaa gttagtgatt gaaaatcggt gctaccaaac gccccagttt cgcaaaatgc
      301 atttggagtt ggccaaggtg ggcaaagggt tggatattct ccactgtgta atgtttcctg
      361 agcctttata cggtctacct ttgtttggct gtgacattgt ggccggcccc ggtggagtaa
      421 gtgcggctat tgcggatcta tcccccaccc aaagcgatcg ccaattgccc gcagcgtacc
      481 aaaaatcatt ggcagagcta ggccagccag aatttgagca acaacgggaa ttgcccccct
      541 ggggagaaat attttctgaa tattgtttat tcatccgtcc cagcaatgtc actgaagaag
      601 aaagatttgt acaaagggta gtggactttt tgcaaattca ttgtcaccaa tccatcgttg
      661 ccgaaccctt gtctgaagct caaactttgg agcaccgtca ggggcaaatt cattactgcc
      721 aacaacaaca gaaaaatgat aaaacccgtc gggtactgga aaaagctttt ggggaagctt
      781 gggcggaacg gtatatgagc caagtcttat ttgatgttat ccaataataa ctctgatagt
      841 gctagtgtag atctctacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga
      901 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg
      961 ctcaccttcg ggtgggcctt tctgcgttta ta
//