LOCUS BBa_J13025 966 bp DNA linear 22-Nov-2024 DEFINITION 3OC6HSL+LuxR dependent mRFP generator ACCESSION BBa_J13025 VERSION BBa_J13025.1 FEATURES Location/Qualifiers misc_feature 1..97 /label=BBa_R0065 misc_feature 6..25 /label=Lux Box promoter 47..52 /label=-10 misc_feature 57..73 /label=OR2 cI promoter 58..58 /label=lux p(R) start promoter 71..76 /label=-35 misc_feature 81..97 /label=OR1 cI promoter 94..97 /label=-10 promoter 1..97 /label=BBa_R0065 misc_feature 110..113 /label=conserved RBS 106..117 /label=BBa_B0034 CDS 124..798 /label=mrfp1 misc_feature 805..829 /label=Help:Barcodes CDS 124..804 /label=BBa_E1010 stem_loop 849..892 /label=stem_loop misc_feature 838..917 /label=BBa_B0010 terminator 838..917 /label=BBa_B0010 stem_loop 933..952 /label=T7 TE misc_feature 959..959 /label=stop polyA_site 953..966 /label=polya misc_feature 926..966 /label=BBa_B0012 terminator 926..966 /label=BBa_B0012 ORIGIN 1 taagcacctg taggatcgta caggtttacg caagaaaatg gtttgttata gtcgaataac 61 accgtgcgtg ttgactattt tacctctggc ggtgatatac tagagaaaga ggagaaatac 121 tagatggctt cctccgaaga cgttatcaaa gagttcatgc gtttcaaagt tcgtatggaa 181 ggttccgtta acggtcacga gttcgaaatc gaaggtgaag gtgaaggtcg tccgtacgaa 241 ggtacccaga ccgctaaact gaaagttacc aaaggtggtc cgctgccgtt cgcttgggac 301 atcctgtccc cgcagttcca gtacggttcc aaagcttacg ttaaacaccc ggctgacatc 361 ccggactacc tgaaactgtc cttcccggaa ggtttcaaat gggaacgtgt tatgaacttc 421 gaagacggtg gtgttgttac cgttacccag gactcctccc tgcaagacgg tgagttcatc 481 tacaaagtta aactgcgtgg taccaacttc ccgtccgacg gtccggttat gcagaaaaaa 541 accatgggtt gggaagcttc caccgaacgt atgtacccgg aagacggtgc tctgaaaggt 601 gaaatcaaaa tgcgtctgaa actgaaagac ggtggtcact acgacgctga agttaaaacc 661 acctacatgg ctaaaaaacc ggttcagctg ccgggtgctt acaaaaccga catcaaactg 721 gacatcacct cccacaacga agactacacc atcgttgaac agtacgaacg tgctgaaggt 781 cgtcactcca ccggtgctta ataacgctga tagtgctagt gtagatcgct actagagcca 841 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt 901 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg 961 tttata //