##gff-version 3
##sequence-region BBa_J13031 1 3863
BBa_J13031	.	terminator	2875	2915	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J13031	.	engineered_region	2875	2915	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J13031	.	stop_codon	2908	2908	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J13031	.	stem_loop	2882	2901	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J13031	.	polyA_site	2902	2915	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J13031	.	ribosome_entry_site	2094	2105	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J13031	.	conserved_region	2098	2101	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J13031	.	terminator	1781	1860	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J13031	.	stem_loop	1792	1835	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J13031	.	engineered_region	1781	1860	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J13031	.	ribosome_entry_site	2986	2997	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J13031	.	conserved_region	2990	2993	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J13031	.	terminator	2787	2866	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J13031	.	stem_loop	2798	2841	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J13031	.	engineered_region	2787	2866	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J13031	.	promoter	1918	2085	.	+	0	ID=BBa_I14016;Name=BBa_I14016
BBa_J13031	.	promoter	2034	2039	.	+	0	ID=annotation1026124;Name=-35;Parent=BBa_I14016
BBa_J13031	.	non_covalent_binding_site	2069	2085	.	+	0	ID=annotation1017878;Name=CI lambda O1;Parent=BBa_I14016
BBa_J13031	.	promoter	2057	2062	.	+	0	ID=annotation1026125;Name=-10;Parent=BBa_I14016
BBa_J13031	.	engineered_region	1918	2068	.	+	0	ID=annotation1017859;Name=R0079;Parent=BBa_I14016
BBa_J13031	.	CDS	124	741	.	+	0	ID=BBa_C0061;Name=BBa_C0061
BBa_J13031	.	engineered_region	124	741	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_J13031	.	tag	703	734	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_J13031	.	engineered_tag	742	766	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_J13031	.	CDS	124	702	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_J13031	.	CDS	992	1747	.	+	0	ID=BBa_C0062;Name=BBa_C0062
BBa_J13031	.	CDS	992	1741	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_J13031	.	engineered_region	992	1747	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_J13031	.	engineered_tag	1748	1772	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_J13031	.	point_mutation	1165	1165	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_J13031	.	engineered_region	992	993	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_J13031	.	point_mutation	1483	1483	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_J13031	.	promoter	2924	2977	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_J13031	.	promoter	2943	2948	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J13031	.	non_covalent_binding_site	2949	2967	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J13031	.	engineered_region	2924	2977	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J13031	.	non_covalent_binding_site	2924	2942	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J13031	.	promoter	2966	2971	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J13031	.	promoter	912	965	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_J13031	.	promoter	931	936	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J13031	.	non_covalent_binding_site	937	955	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J13031	.	engineered_region	912	965	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J13031	.	non_covalent_binding_site	912	930	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J13031	.	promoter	954	959	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J13031	.	ribosome_entry_site	974	985	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J13031	.	conserved_region	978	981	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J13031	.	CDS	3004	3726	.	+	0	ID=BBa_C0179;Name=BBa_C0179
BBa_J13031	.	terminator	863	903	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J13031	.	engineered_region	863	903	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J13031	.	stop_codon	896	896	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J13031	.	stem_loop	870	889	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J13031	.	polyA_site	890	903	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J13031	.	ribosome_entry_site	106	117	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J13031	.	conserved_region	110	113	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J13031	.	promoter	1	97	.	+	0	ID=BBa_R0065;Name=BBa_R0065
BBa_J13031	.	promoter	94	97	.	+	0	ID=annotation1986781;Name=-10;Parent=BBa_R0065
BBa_J13031	.	non_covalent_binding_site	81	97	.	+	0	ID=annotation1986780;Name=OR1 cI;Parent=BBa_R0065
BBa_J13031	.	promoter	47	52	.	+	0	ID=annotation1986776;Name=-10;Parent=BBa_R0065
BBa_J13031	.	non_covalent_binding_site	6	25	.	+	0	ID=annotation1986775;Name=Lux Box;Parent=BBa_R0065
BBa_J13031	.	promoter	71	76	.	+	0	ID=annotation1986779;Name=-35;Parent=BBa_R0065
BBa_J13031	.	promoter	58	58	.	+	0	ID=annotation1986778;Name=lux p(R) start;Parent=BBa_R0065
BBa_J13031	.	non_covalent_binding_site	57	73	.	+	0	ID=annotation1986777;Name=OR2 cI;Parent=BBa_R0065
BBa_J13031	.	engineered_region	1	97	.	+	0	ID=annotation1986774;Name=BBa_R0065;Parent=BBa_R0065
BBa_J13031	.	terminator	3735	3814	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J13031	.	stem_loop	3746	3789	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J13031	.	engineered_region	3735	3814	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J13031	.	terminator	1869	1909	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J13031	.	engineered_region	1869	1909	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J13031	.	stop_codon	1902	1902	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J13031	.	stem_loop	1876	1895	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J13031	.	polyA_site	1896	1909	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J13031	.	terminator	775	854	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J13031	.	stem_loop	786	829	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J13031	.	engineered_region	775	854	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J13031	.	terminator	3823	3863	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J13031	.	engineered_region	3823	3863	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J13031	.	stop_codon	3856	3856	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J13031	.	stem_loop	3830	3849	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J13031	.	polyA_site	3850	3863	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J13031	.	CDS	2112	2753	.	+	0	ID=BBa_C0078;Name=BBa_C0078
BBa_J13031	.	tag	2715	2747	.	+	0	ID=annotation306608;Name=LVA;Parent=BBa_C0078
BBa_J13031	.	engineered_tag	2754	2778	.	+	0	ID=annotation2214003;Name=Help:Barcodes;Parent=BBa_C0078
BBa_J13031	.	CDS	2112	2714	.	+	0	ID=annotation305970;Name=LasI;Parent=BBa_C0078
>BBa_J13031
taagcacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataacaccgtgcgtgttgactatt
ttacctctggcggtgatatactagagaaagaggagaaatactagatgactataatgataaaaaaatcggattttttggc
aattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagtt
gtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatg
taagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaaca
gagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactct
gctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacag
taacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgt
attaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaac
gacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaa
acgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacact
ggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtg
atagagatactgagcactactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaata
attaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtg
aatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaa
atggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcacca
attaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtc
ttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaaga
caactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcga
aaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaag
gaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaat
gaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaa
aattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaaga
ctgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcct
ttctgcgtttatatactagaggcccctcgctgagcgcgtcccggagctgggggcaacctagctgccacctgcttttctg
ctagctattccagcgaaaacatacagatttccggcgaaatcaaggctacctgccagttctggcaggtttggccgcgggt
tctttttggtacactacctctggcggtgatatactagagaaagaggagaaatactagatgatcgttcagatcggtcgtc
gtgaagagttcgacaaaaaactgctgggtgaaatgcacaaactgcgtgctcaggttttcaaagaacgtaaaggttggga
cgtttccgttatcgacgaaatggaaatcgacggttacgacgctctgtccccgtactacatgctgatccaggaagacacc
ccggaagctcaggttttcggttgctggcgtatcttcgacaccaccggtccgtacatgctgaaaaacaccttcccggaac
tgctgcacggtaaagaagctccgtgctccccgcacatctgggaactgtcccgtttcgctatcaactccggtcagaaagg
ttccctgggtttctccgactgcaccctggaagctatgcgtgctctggctcgttactccttgcagaacgacatccagacc
ctggttaccgttaccaccgttggtgttgaaaaaatgatgatccgtgctggtctggacgtttcccgtttcggtccgcacc
tgaaaatcggtatcgaacgtgctgttgctctgcgtatcgaactgaacgctaaaacccagatcgctctgtacggtggtgt
tctggttgaacagcgtctggctgtttccgctgctaacgacgaaaactacgctctggttgcttaataactctgatagtgc
tagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctg
ttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagag
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatggccttggttgacggttttcttgagctggaacgctcaagtggaaaattggagtggagcgccatcctccagaagatg
gcgagcgaccttggattctcgaagatcctgttcggcctgttgcctaaggacagccaggactacgagaacgccttcatcg
tcggcaactacccggccgcctggcgcgagcattacgaccgggctggctacgcgcgggtcgacccgacggtcagtcactg
tacccagagcgtactgccgattttctgggaaccgtccatctaccagacgcgaaagcagcacgagttcttcgaggaagcc
tcggccgccggcctggtgtatgggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgctgagcctcagcg
tggaagcggaaaaccgggccgaggccaaccgtttcatagagtcggtcctgccgaccctgtggatgctcaaggactacgc
actgcaaagcggtgccggactggccttcgaacatccggtcagcaaaccggtggttctgaccagccgggagaaggaagtg
ttgcagtggtgcgccatcggcaagaccagttgggagatatcggttatctgcaactgctcggaagccaatgtgaacttcc
atatgggaaatattcggcggaagttcggtgtgacctcccgccgcgtagcggccattatggccgttaatttgggtcttat
tactctctaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctg
ttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata