LOCUS       BBa_J13065              2025 bp    DNA     linear       22-Nov-2024
DEFINITION  Monocistronic CFP/YFP expression cassette
ACCESSION   BBa_J13065
VERSION     BBa_J13065.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..803
                     /label=BBa_E0020
     stem_loop       823..866
                     /label=stem_loop
     misc_feature    812..891
                     /label=BBa_B0010
     terminator      812..891
                     /label=BBa_B0010
     stem_loop       907..926
                     /label=T7 TE
     misc_feature    933..933
                     /label=stop
     polyA_site      927..940
                     /label=polya
     misc_feature    900..940
                     /label=BBa_B0012
     terminator      900..940
                     /label=BBa_B0012
     stem_loop       960..1003
                     /label=stem_loop
     misc_feature    949..1028
                     /label=BBa_B0010
     terminator      949..1028
                     /label=BBa_B0010
     stem_loop       1044..1063
                     /label=T7 TE
     misc_feature    1070..1070
                     /label=stop
     polyA_site      1064..1077
                     /label=polya
     misc_feature    1037..1077
                     /label=BBa_B0012
     terminator      1037..1077
                     /label=BBa_B0012
     misc_feature    1086..1104
                     /label=TetR 1
     misc_feature    1086..1139
                     /label=BBa_R0040
     promoter        1105..1110
                     /label=-35
     misc_feature    1111..1129
                     /label=TetR 2
     promoter        1128..1133
                     /label=-10
     promoter        1086..1139
                     /label=BBa_R0040
     misc_feature    1152..1155
                     /label=conserved
     RBS             1148..1159
                     /label=BBa_B0034
     CDS             1166..1888
                     /label=BBa_E0030
     stem_loop       1908..1951
                     /label=stem_loop
     misc_feature    1897..1976
                     /label=BBa_B0010
     terminator      1897..1976
                     /label=BBa_B0010
     stem_loop       1992..2011
                     /label=T7 TE
     misc_feature    2018..2018
                     /label=stop
     polyA_site      2012..2025
                     /label=polya
     misc_feature    1985..2025
                     /label=BBa_B0012
     terminator      1985..2025
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atggtgagca agggcgagga gctgttcacc ggggtggtgc
      121 ccatcctggt cgagctggac ggcgacgtga acggccacaa gttcagcgtg tccggcgagg
      181 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
      241 tgcccgtgcc ctggcccacc ctcgtgacca ccctgacctg gggcgtgcag tgcttcagcc
      301 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
      361 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
      421 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
      481 acggcaacat cctggggcac aagctggagt acaactacat cagccacaac gtctatatca
      541 ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa gatccgccac aacatcgagg
      601 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
      661 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg
      721 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
      781 tggacgagct gtacaagtaa taatactaga gccaggcatc aaataaaacg aaaggctcag
      841 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
      901 cacactggct caccttcggg tgggcctttc tgcgtttata tactagagcc aggcatcaaa
      961 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
     1021 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac
     1081 tagagtccct atcagtgata gagattgaca tccctatcag tgatagagat actgagcact
     1141 actagagaaa gaggagaaat actagatggt gagcaagggc gaggagctgt tcaccggggt
     1201 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     1261 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     1321 caagctgccc gtgccctggc ccaccctcgt gaccaccttc ggctacggcc tgcaatgctt
     1381 cgcccgctac cccgaccaca tgaagctgca cgacttcttc aagtccgcca tgcccgaagg
     1441 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     1501 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     1561 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     1621 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat
     1681 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     1741 ccccgtgctg ctgcccgaca accactacct gagctaccag tccgccctga gcaaagaccc
     1801 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     1861 cggcatggac gagctgtaca agtaataata ctagagccag gcatcaaata aaacgaaagg
     1921 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
     1981 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata
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