##gff-version 3
##sequence-region BBa_J13068 1 2757
BBa_J13068	.	engineered_region	1897	2757	.	+	0	ID=BBa_I13507;Name=BBa_I13507
BBa_J13068	.	ribosome_entry_site	1897	1908	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13507
BBa_J13068	.	conserved_region	1901	1904	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J13068	.	terminator	2717	2757	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13507
BBa_J13068	.	polyA_site	2744	2757	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J13068	.	engineered_region	2717	2757	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J13068	.	stem_loop	2724	2743	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J13068	.	stop_codon	2750	2750	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J13068	.	terminator	2629	2708	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13507
BBa_J13068	.	stem_loop	2640	2683	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J13068	.	engineered_region	2629	2708	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J13068	.	CDS	1915	2595	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13507
BBa_J13068	.	CDS	1915	2589	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_J13068	.	engineered_tag	2596	2620	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_J13068	.	engineered_region	1	1888	.	+	0	ID=BBa_J13066;Name=BBa_J13066
BBa_J13068	.	CDS	81	803	.	+	0	ID=BBa_E0020;Name=BBa_E0020;Parent=BBa_J13066
BBa_J13068	.	promoter	1835	1888	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_J13066
BBa_J13068	.	engineered_region	1835	1888	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J13068	.	non_covalent_binding_site	1835	1853	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J13068	.	non_covalent_binding_site	1860	1878	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J13068	.	promoter	1854	1859	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J13068	.	promoter	1877	1882	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J13068	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_J13066
BBa_J13068	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J13068	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J13068	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J13068	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J13068	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J13068	.	terminator	1561	1640	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J13066
BBa_J13068	.	stem_loop	1572	1615	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J13068	.	engineered_region	1561	1640	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J13068	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J13066
BBa_J13068	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J13068	.	CDS	830	1552	.	+	0	ID=BBa_E0030;Name=BBa_E0030;Parent=BBa_J13066
BBa_J13068	.	terminator	1786	1826	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J13066
BBa_J13068	.	polyA_site	1813	1826	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J13068	.	engineered_region	1786	1826	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J13068	.	stem_loop	1793	1812	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J13068	.	stop_codon	1819	1819	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J13068	.	terminator	1698	1777	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J13066
BBa_J13068	.	stem_loop	1709	1752	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J13068	.	engineered_region	1698	1777	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J13068	.	ribosome_entry_site	812	823	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J13066
BBa_J13068	.	conserved_region	816	819	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J13068	.	terminator	1649	1689	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J13066
BBa_J13068	.	polyA_site	1676	1689	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J13068	.	engineered_region	1649	1689	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J13068	.	stem_loop	1656	1675	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J13068	.	stop_codon	1682	1682	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
>BBa_J13068
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccac
aagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggca
agctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacat
gaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggc
aactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttca
aggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagca
gaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccag
cagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaag
accccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagct
gtacaagtaataatactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgc
ccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccaccta
cggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggc
tacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggct
acgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacac
cctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaac
tacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaaca
tcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccga
caaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttc
gtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacga
aaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggct
caccttcgggtgggcctttctgcgtttatatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgg
gcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttct
gcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagag
aaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggtt
ccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaa
agttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgtt
aaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcg
aagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtgg
taccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccg
gaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaa
ccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaa
cgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgc
tagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctg
ttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata