##gff-version 3
##sequence-region BBa_J13070 1 931
BBa_J13070	.	engineered_region	81	794	.	+	0	ID=BBa_J06504;Name=BBa_J06504
BBa_J13070	.	CDS	81	791	.	+	0	ID=annotation1585829;Name=mCherry;Parent=BBa_J06504
BBa_J13070	.	sequence_alteration	432	432	.	+	0	ID=annotation1585833;Name=C->T (removing PstI site);Parent=BBa_J06504
BBa_J13070	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_J13070	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J13070	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J13070	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J13070	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J13070	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J13070	.	terminator	803	882	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J13070	.	engineered_region	803	882	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J13070	.	stem_loop	814	857	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J13070	.	terminator	891	931	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J13070	.	engineered_region	891	931	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J13070	.	polyA_site	918	931	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J13070	.	stem_loop	898	917	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J13070	.	stop_codon	924	924	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J13070	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J13070	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_J13070
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctcc
gtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaagg
tgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaa
gcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgag
gacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggca
ccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccga
ggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagacc
acctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacg
aggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagta
ataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtc
ggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata