LOCUS BBa_J13065 2025 bp DNA linear 29-Nov-2024 DEFINITION Monocistronic CFP/YFP expression cassette ACCESSION BBa_J13065 VERSION BBa_J13065.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 CDS 81..803 /label=BBa_E0020 stem_loop 823..866 /label=stem_loop misc_feature 812..891 /label=BBa_B0010 terminator 812..891 /label=BBa_B0010 stem_loop 907..926 /label=T7 TE misc_feature 933..933 /label=stop polyA_site 927..940 /label=polya misc_feature 900..940 /label=BBa_B0012 terminator 900..940 /label=BBa_B0012 stem_loop 960..1003 /label=stem_loop misc_feature 949..1028 /label=BBa_B0010 terminator 949..1028 /label=BBa_B0010 stem_loop 1044..1063 /label=T7 TE misc_feature 1070..1070 /label=stop polyA_site 1064..1077 /label=polya misc_feature 1037..1077 /label=BBa_B0012 terminator 1037..1077 /label=BBa_B0012 misc_feature 1086..1104 /label=TetR 1 misc_feature 1086..1139 /label=BBa_R0040 promoter 1105..1110 /label=-35 misc_feature 1111..1129 /label=TetR 2 promoter 1128..1133 /label=-10 promoter 1086..1139 /label=BBa_R0040 misc_feature 1152..1155 /label=conserved RBS 1148..1159 /label=BBa_B0034 CDS 1166..1888 /label=BBa_E0030 stem_loop 1908..1951 /label=stem_loop misc_feature 1897..1976 /label=BBa_B0010 terminator 1897..1976 /label=BBa_B0010 stem_loop 1992..2011 /label=T7 TE misc_feature 2018..2018 /label=stop polyA_site 2012..2025 /label=polya misc_feature 1985..2025 /label=BBa_B0012 terminator 1985..2025 /label=BBa_B0012 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atggtgagca agggcgagga gctgttcacc ggggtggtgc 121 ccatcctggt cgagctggac ggcgacgtga acggccacaa gttcagcgtg tccggcgagg 181 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc 241 tgcccgtgcc ctggcccacc ctcgtgacca ccctgacctg gggcgtgcag tgcttcagcc 301 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg 361 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga 421 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg 481 acggcaacat cctggggcac aagctggagt acaactacat cagccacaac gtctatatca 541 ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa gatccgccac aacatcgagg 601 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg 661 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg 721 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca 781 tggacgagct gtacaagtaa taatactaga gccaggcatc aaataaaacg aaaggctcag 841 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt 901 cacactggct caccttcggg tgggcctttc tgcgtttata tactagagcc aggcatcaaa 961 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga 1021 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac 1081 tagagtccct atcagtgata gagattgaca tccctatcag tgatagagat actgagcact 1141 actagagaaa gaggagaaat actagatggt gagcaagggc gaggagctgt tcaccggggt 1201 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg 1261 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg 1321 caagctgccc gtgccctggc ccaccctcgt gaccaccttc ggctacggcc tgcaatgctt 1381 cgcccgctac cccgaccaca tgaagctgca cgacttcttc aagtccgcca tgcccgaagg 1441 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga 1501 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa 1561 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta 1621 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat 1681 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg 1741 ccccgtgctg ctgcccgaca accactacct gagctaccag tccgccctga gcaaagaccc 1801 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct 1861 cggcatggac gagctgtaca agtaataata ctagagccag gcatcaaata aaacgaaagg 1921 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact 1981 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata // LOCUS BBa_J13076 2025 bp DNA linear 29-Nov-2024 DEFINITION Monocistronic CFP/YFP expression cassette ACCESSION BBa_J13076 VERSION BBa_J13076.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 CDS 81..803 /label=BBa_E0020 stem_loop 823..866 /label=stem_loop misc_feature 812..891 /label=BBa_B0010 terminator 812..891 /label=BBa_B0010 stem_loop 907..926 /label=T7 TE misc_feature 933..933 /label=stop polyA_site 927..940 /label=polya misc_feature 900..940 /label=BBa_B0012 terminator 900..940 /label=BBa_B0012 stem_loop 960..1003 /label=stem_loop misc_feature 949..1028 /label=BBa_B0010 terminator 949..1028 /label=BBa_B0010 stem_loop 1044..1063 /label=T7 TE misc_feature 1070..1070 /label=stop polyA_site 1064..1077 /label=polya misc_feature 1037..1077 /label=BBa_B0012 terminator 1037..1077 /label=BBa_B0012 misc_feature 1086..1104 /label=TetR 1 misc_feature 1086..1139 /label=BBa_R0040 promoter 1105..1110 /label=-35 misc_feature 1111..1129 /label=TetR 2 promoter 1128..1133 /label=-10 promoter 1086..1139 /label=BBa_R0040 misc_feature 1152..1155 /label=conserved RBS 1148..1159 /label=BBa_B0034 CDS 1166..1888 /label=BBa_E0030 stem_loop 1908..1951 /label=stem_loop misc_feature 1897..1976 /label=BBa_B0010 terminator 1897..1976 /label=BBa_B0010 stem_loop 1992..2011 /label=T7 TE misc_feature 2018..2018 /label=stop polyA_site 2012..2025 /label=polya misc_feature 1985..2025 /label=BBa_B0012 terminator 1985..2025 /label=BBa_B0012 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atggtgagca agggcgagga gctgttcacc ggggtggtgc 121 ccatcctggt cgagctggac ggcgacgtga acggccacaa gttcagcgtg tccggcgagg 181 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc 241 tgcccgtgcc ctggcccacc ctcgtgacca ccctgacctg gggcgtgcag tgcttcagcc 301 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg 361 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga 421 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg 481 acggcaacat cctggggcac aagctggagt acaactacat cagccacaac gtctatatca 541 ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa gatccgccac aacatcgagg 601 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg 661 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg 721 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca 781 tggacgagct gtacaagtaa taatactaga gccaggcatc aaataaaacg aaaggctcag 841 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt 901 cacactggct caccttcggg tgggcctttc tgcgtttata tactagagcc aggcatcaaa 961 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga 1021 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac 1081 tagagtccct atcagtgata gagattgaca tccctatcag tgatagagat actgagcact 1141 actagagaaa gaggagaaat actagatggt gagcaagggc gaggagctgt tcaccggggt 1201 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg 1261 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg 1321 caagctgccc gtgccctggc ccaccctcgt gaccaccttc ggctacggcc tgcaatgctt 1381 cgcccgctac cccgaccaca tgaagctgca cgacttcttc aagtccgcca tgcccgaagg 1441 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga 1501 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa 1561 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta 1621 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat 1681 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg 1741 ccccgtgctg ctgcccgaca accactacct gagctaccag tccgccctga gcaaagaccc 1801 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct 1861 cggcatggac gagctgtaca agtaataata ctagagccag gcatcaaata aaacgaaagg 1921 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact 1981 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata //