LOCUS       BBa_J13081              2008 bp    DNA     linear       19-Dec-2024
DEFINITION  Monocistronic RFP/YFP expression cassette
ACCESSION   BBa_J13081
VERSION     BBa_J13081.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..803
                     /label=BBa_E0030
     stem_loop       823..866
                     /label=stem_loop
     misc_feature    812..891
                     /label=BBa_B0010
     terminator      812..891
                     /label=BBa_B0010
     stem_loop       907..926
                     /label=T7 TE
     misc_feature    933..933
                     /label=stop
     polyA_site      927..940
                     /label=polya
     misc_feature    900..940
                     /label=BBa_B0012
     terminator      900..940
                     /label=BBa_B0012
     stem_loop       960..1003
                     /label=stem_loop
     misc_feature    949..1028
                     /label=BBa_B0010
     terminator      949..1028
                     /label=BBa_B0010
     stem_loop       1044..1063
                     /label=T7 TE
     misc_feature    1070..1070
                     /label=stop
     polyA_site      1064..1077
                     /label=polya
     misc_feature    1037..1077
                     /label=BBa_B0012
     terminator      1037..1077
                     /label=BBa_B0012
     misc_feature    1086..1104
                     /label=TetR 1
     misc_feature    1086..1139
                     /label=BBa_R0040
     promoter        1105..1110
                     /label=-35
     misc_feature    1111..1129
                     /label=TetR 2
     promoter        1128..1133
                     /label=-10
     promoter        1086..1139
                     /label=BBa_R0040
     misc_feature    1..1139
                     /label=BBa_J13063
     misc_feature    1152..1155
                     /label=conserved
     RBS             1148..1159
                     /label=BBa_B0034
     CDS             1166..1840
                     /label=mrfp1
     misc_feature    1847..1871
                     /label=Help:Barcodes
     CDS             1166..1846
                     /label=BBa_E1010
     stem_loop       1891..1934
                     /label=stem_loop
     misc_feature    1880..1959
                     /label=BBa_B0010
     terminator      1880..1959
                     /label=BBa_B0010
     stem_loop       1975..1994
                     /label=T7 TE
     misc_feature    2001..2001
                     /label=stop
     polyA_site      1995..2008
                     /label=polya
     misc_feature    1968..2008
                     /label=BBa_B0012
     terminator      1968..2008
                     /label=BBa_B0012
     misc_feature    1148..2008
                     /label=BBa_I13507
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atggtgagca agggcgagga gctgttcacc ggggtggtgc
      121 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
      181 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
      241 tgcccgtgcc ctggcccacc ctcgtgacca ccttcggcta cggcctgcaa tgcttcgccc
      301 gctaccccga ccacatgaag ctgcacgact tcttcaagtc cgccatgccc gaaggctacg
      361 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
      421 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
      481 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
      541 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg
      601 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
      661 tgctgctgcc cgacaaccac tacctgagct accagtccgc cctgagcaaa gaccccaacg
      721 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
      781 tggacgagct gtacaagtaa taatactaga gccaggcatc aaataaaacg aaaggctcag
      841 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
      901 cacactggct caccttcggg tgggcctttc tgcgtttata tactagagcc aggcatcaaa
      961 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
     1021 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac
     1081 tagagtccct atcagtgata gagattgaca tccctatcag tgatagagat actgagcact
     1141 actagagaaa gaggagaaat actagatggc ttcctccgaa gacgttatca aagagttcat
     1201 gcgtttcaaa gttcgtatgg aaggttccgt taacggtcac gagttcgaaa tcgaaggtga
     1261 aggtgaaggt cgtccgtacg aaggtaccca gaccgctaaa ctgaaagtta ccaaaggtgg
     1321 tccgctgccg ttcgcttggg acatcctgtc cccgcagttc cagtacggtt ccaaagctta
     1381 cgttaaacac ccggctgaca tcccggacta cctgaaactg tccttcccgg aaggtttcaa
     1441 atgggaacgt gttatgaact tcgaagacgg tggtgttgtt accgttaccc aggactcctc
     1501 cctgcaagac ggtgagttca tctacaaagt taaactgcgt ggtaccaact tcccgtccga
     1561 cggtccggtt atgcagaaaa aaaccatggg ttgggaagct tccaccgaac gtatgtaccc
     1621 ggaagacggt gctctgaaag gtgaaatcaa aatgcgtctg aaactgaaag acggtggtca
     1681 ctacgacgct gaagttaaaa ccacctacat ggctaaaaaa ccggttcagc tgccgggtgc
     1741 ttacaaaacc gacatcaaac tggacatcac ctcccacaac gaagactaca ccatcgttga
     1801 acagtacgaa cgtgctgaag gtcgtcactc caccggtgct taataacgct gatagtgcta
     1861 gtgtagatcg ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
     1921 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca
     1981 ccttcgggtg ggcctttctg cgtttata
//