LOCUS       BBa_J13202              1981 bp    DNA     linear       13-May-2024
DEFINITION  3OC6HSL + LuxR reporter and aiia (LVA+) tuning device
ACCESSION   BBa_J13202
VERSION     BBa_J13202.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=LuxR/HSL
     promoter        20..25
                     /label=-35
     promoter        42..47
                     /label=-10
     misc_feature    53..53
                     /label=start
     misc_feature    1..55
                     /label=BBa_R0062
     promoter        1..55
                     /label=BBa_R0062
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     CDS             82..804
                     /label=BBa_E0030
     stem_loop       824..867
                     /label=stem_loop
     misc_feature    813..892
                     /label=BBa_B0010
     terminator      813..892
                     /label=BBa_B0010
     stem_loop       908..927
                     /label=T7 TE
     misc_feature    934..934
                     /label=stop
     polyA_site      928..941
                     /label=polya
     misc_feature    901..941
                     /label=BBa_B0012
     terminator      901..941
                     /label=BBa_B0012
     misc_feature    952..968
                     /label=lac O1
     promoter        969..974
                     /label=-35
     misc_feature    975..991
                     /label=lac O1
     promoter        992..997
                     /label=-10
     misc_feature    950..1003
                     /label=BBa_R0011
     promoter        950..1003
                     /label=BBa_R0011
     misc_feature    1017..1020
                     /label=conserved
     RBS             1013..1024
                     /label=BBa_B0034
     misc_feature    1031..1033
                     /label=start
     misc_feature    1814..1819
                     /label=2
     misc_feature    1781..1813
                     /label=LVA
     CDS             1031..1780
                     /label=aiiA
     misc_feature    1031..1819
                     /label=BBa_C0060
     misc_feature    1820..1844
                     /label=Help:Barcodes
     CDS             1031..1819
                     /label=BBa_C0060
     stem_loop       1864..1907
                     /label=stem_loop
     misc_feature    1853..1932
                     /label=BBa_B0010
     terminator      1853..1932
                     /label=BBa_B0010
     stem_loop       1948..1967
                     /label=T7 TE
     misc_feature    1974..1974
                     /label=stop
     polyA_site      1968..1981
                     /label=polya
     misc_feature    1941..1981
                     /label=BBa_B0012
     terminator      1941..1981
                     /label=BBa_B0012
ORIGIN
        1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta
       61 gagaaagagg agaaatacta gatggtgagc aagggcgagg agctgttcac cggggtggtg
      121 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
      181 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
      241 ctgcccgtgc cctggcccac cctcgtgacc accttcggct acggcctgca atgcttcgcc
      301 cgctaccccg accacatgaa gctgcacgac ttcttcaagt ccgccatgcc cgaaggctac
      361 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
      421 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
      481 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
      541 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
      601 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
      661 gtgctgctgc ccgacaacca ctacctgagc taccagtccg ccctgagcaa agaccccaac
      721 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
      781 atggacgagc tgtacaagta ataatactag agccaggcat caaataaaac gaaaggctca
      841 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag
      901 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagaga attgtgagcg
      961 gataacaatt gacattgtga gcggataaca agatactgag cacatactag agaaagagga
     1021 gaaatactag atgacagtaa agaagcttta tttcgtccca gcaggtcgtt gtatgttgga
     1081 tcattcgtct gttaatagta cattaacacc aggagaatta ttagacttac cggtttggtg
     1141 ttatcttttg gagactgaag aaggacctat tttagtagat acaggtatgc cagaaagtgc
     1201 agttaataat gaaggtcttt ttaacggtac atttgtcgaa gggcaggttt taccgaaaat
     1261 gactgaagaa gatagaatcg tgaatatttt aaaacgggtt ggttatgagc cggaagacct
     1321 tctttatatt attagttctc acttgcattt tgatcatgca ggaggaaatg gcgcttttat
     1381 aaatacacca atcattgtac agcgtgctga atatgaggcg gcgcagcata gcgaagaata
     1441 tttgaaagaa tgtatattgc cgaatttaaa ctacaaaatc attgaaggtg attatgaagt
     1501 cgtaccagga gttcaattat tgcatacacc aggccatact ccagggcatc aatcgctatt
     1561 aattgagaca gaaaaatccg gtcctgtatt attaacgatt gatgcatcgt atacgaaaga
     1621 gaattttgaa aatgaagtgc catttgcggg atttgattca gaattagctt tatcttcaat
     1681 taaacgttta aaagaagtgg tgatgaaaga gaagccgatt gttttctttg gacatgatat
     1741 agagcaggaa aggggatgta aagtgttccc tgaatatata gctgcaaacg acgaaaacta
     1801 cgctttagta gcttaataac gctgatagtg ctagtgtaga tcgctactag agccaggcat
     1861 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
     1921 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat
     1981 a
//