LOCUS       BBa_J13204              1805 bp    DNA     linear       22-Dec-2024
DEFINITION  3OC6HSL + LuxR reporter and cI tuning device
ACCESSION   BBa_J13204
VERSION     BBa_J13204.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=LuxR/HSL
     promoter        20..25
                     /label=-35
     promoter        42..47
                     /label=-10
     misc_feature    53..53
                     /label=start
     misc_feature    1..55
                     /label=BBa_R0062
     promoter        1..55
                     /label=BBa_R0062
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     CDS             82..804
                     /label=BBa_E0030
     stem_loop       824..867
                     /label=stem_loop
     misc_feature    813..892
                     /label=BBa_B0010
     terminator      813..892
                     /label=BBa_B0010
     stem_loop       908..927
                     /label=T7 TE
     misc_feature    934..934
                     /label=stop
     polyA_site      928..941
                     /label=polya
     misc_feature    901..941
                     /label=BBa_B0012
     terminator      901..941
                     /label=BBa_B0012
     misc_feature    952..968
                     /label=lac O1
     promoter        969..974
                     /label=-35
     misc_feature    975..991
                     /label=lac O1
     promoter        992..997
                     /label=-10
     misc_feature    950..1003
                     /label=BBa_R0011
     promoter        950..1003
                     /label=BBa_R0011
     misc_feature    1017..1020
                     /label=conserved
     RBS             1013..1024
                     /label=BBa_B0034
     CDS             1034..1741
                     /label=cI lambda
     misc_feature    1742..1774
                     /label=LVA
     misc_feature    1781..1805
                     /label=Help:Barcodes
     CDS             1031..1780
                     /label=BBa_C0051
ORIGIN
        1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta
       61 gagaaagagg agaaatacta gatggtgagc aagggcgagg agctgttcac cggggtggtg
      121 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
      181 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
      241 ctgcccgtgc cctggcccac cctcgtgacc accttcggct acggcctgca atgcttcgcc
      301 cgctaccccg accacatgaa gctgcacgac ttcttcaagt ccgccatgcc cgaaggctac
      361 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
      421 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
      481 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
      541 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
      601 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
      661 gtgctgctgc ccgacaacca ctacctgagc taccagtccg ccctgagcaa agaccccaac
      721 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
      781 atggacgagc tgtacaagta ataatactag agccaggcat caaataaaac gaaaggctca
      841 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag
      901 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagaga attgtgagcg
      961 gataacaatt gacattgtga gcggataaca agatactgag cacatactag agaaagagga
     1021 gaaatactag atgagcacaa aaaagaaacc attaacacaa gagcagcttg aggacgcacg
     1081 tcgccttaaa gcaatttatg aaaaaaagaa aaatgaactt ggcttatccc aggaatctgt
     1141 cgcagacaag atggggatgg ggcagtcagg cgttggtgct ttatttaatg gcatcaatgc
     1201 attaaatgct tataacgccg cattgcttgc aaaaattctc aaagttagcg ttgaagaatt
     1261 tagcccttca atcgccagag aaatctacga gatgtatgaa gcggttagta tgcagccgtc
     1321 acttagaagt gagtatgagt accctgtttt ttctcatgtt caggcaggga tgttctcacc
     1381 tgagcttaga acctttacca aaggtgatgc ggagagatgg gtaagcacaa ccaaaaaagc
     1441 cagtgattct gcattctggc ttgaggttga aggtaattcc atgaccgcac caacaggctc
     1501 caagccaagc tttcctgacg gaatgttaat tctcgttgac cctgagcagg ctgttgagcc
     1561 aggtgatttc tgcatagcca gacttggggg tgatgagttt accttcaaga aactgatcag
     1621 ggatagcggt caggtgtttt tacaaccact aaacccacag tacccaatga tcccatgcaa
     1681 tgagagttgt tccgttgtgg ggaaagttat cgctagtcag tggcctgaag agacgtttgg
     1741 cgctgcaaac gacgaaaact acgctttagt agcttaataa cgctgatagt gctagtgtag
     1801 atcgc
//