BBa_J133002 1 BBa_J133002 GFPFrag+OligoN 2010-12-17T12:00:00Z 2015-08-31T04:08:30Z Source DNA: backbone: PCRed using G1000-G1001 from pSB3K5-J732720 insert1: PCRed using custom mut primers g0001_f-g0002_r from E0240 E Coli cryostock insert2: insert freed from pIDTSMART-OligoN plasmid prep aka GFPFrag + OligoN Merger of 2 Parts: 1.) PCRed B0034+E0040 5' fragment from E0240 with mutagenesis primers to remove BsmBI 2.) 326bp Synthetic fragment (OligoN) to include: a.) 3' fragment of E0040, removing BsaI and b.) RNAseE+NheI+NsiI+RNAseE+Hairpin (reverse engineered from pSB1A10) false false _634_ 0 2680 9 Not in stock false gene synthesis from IDTDNA, $220 (max 400bp), ordered on 9/22/10, arrived 10/27/10 false Norman Wang BBa_J133002_sequence 1 tactagagcgtctccaattggcggccgccctaggtactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaatcggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagatcacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagtattatttgtattgatctggtgaattaggtcttcgaatcgctagcgcggccgcatgcatgagacccgatccattgcagtggtattatttgtattgatctccttactatctctcgagagattagtacctttggagatc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z