BBa_J133650
1
BBa_J133650
Chimerabrick Replacement Cassette
2011-02-17T12:00:00Z
2015-08-31T04:08:30Z
n/a
Synthesized oligo intended to fix the original Screening Plasmid 3.0 design
from >MA reporterA NI< reporterB
to reporterA >MA NI< reporterB
The oligos encode Chimerabrick Replacement Cassette insert (cut X N):
X rE sP1 > M A N
Replaces X to N region in current construct (cut X N):
E X > M A B0032 GFP rE sP1 N I < sP2 rE Hr S P
to:
E X rE sP1 > M A N I < sP2 rE Hr S P
Legend:
> = BsmBI
M = MfeI
A = AvrII
N = NheI
I = NsiI
> = BsaI
rE = rnaseE site
sP1 = spacer 1
sp2 = spacer 2
rE = rnaseE site
Hr = mRNA stabilizing hairpin
false
false
_634_
0
2680
9
Not in stock
false
n/a
false
Norman Wang
BBa_J133650_sequence
1
ctagagtattatttgtattgatctggtgaattaggtcttcgaatccgtctccaattggcggccgccctagggctagcgcggccgcatgca
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z