LOCUS       BBa_J133653              859 bp    DNA     linear       22-Dec-2024
DEFINITION  aadA Cassette + Flanked by TLTerminators (missing RBS)
ACCESSION   BBa_J133653
VERSION     BBa_J133653.1  
FEATURES             Location/Qualifiers
     misc_feature    1..12
                     /label=BBa_J133422
     promoter        13..49
                     /label=BBa I14032 P(Lac) IQ
     misc_feature    56..847
                     /label=BBa K125520 Aminoglycoside 3"-Adenylyltransferase 
                     (aadA1) Gene CDS
     misc_feature    848..859
                     /label=BBa_B0042
     misc_feature    296..298
                     /label=E81V mutation GAG to GTG
ORIGIN
        1 ttagctaatt agtggtgcaa aacctttcgc ggtatggcat gatagcgcct actagatgag
       61 ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg tcatcgagcg
      121 ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg atggcggcct
      181 gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg atgaaacaac
      241 gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag agagcgtgat
      301 tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt ggcgttatcc
      361 agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg caggtatctt
      421 cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa gagaacatag
      481 cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg aacaggatct
      541 atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact gggctggcga
      601 tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa ccggcaaaat
      661 cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc agtatcagcc
      721 cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct tggcctcgcg
      781 cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg
      841 caaataatta gttagttag
//