LOCUS       BBa_J14018              1089 bp    DNA     linear       15-May-2024
DEFINITION  Terminator that terminates well
ACCESSION   BBa_J14018
VERSION     BBa_J14018.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_J04500
     misc_feature    233..236
                     /label=conserved
     RBS             229..240
                     /label=BBa_B0034
     CDS             247..921
                     /label=mrfp1
     misc_feature    928..952
                     /label=Help:Barcodes
     CDS             247..927
                     /label=BBa_E1010
     stem_loop       972..1015
                     /label=stem_loop
     misc_feature    961..1040
                     /label=BBa_B0010
     terminator      961..1040
                     /label=BBa_B0010
     stem_loop       1056..1075
                     /label=T7 TE
     misc_feature    1082..1082
                     /label=stop
     polyA_site      1076..1089
                     /label=polya
     misc_feature    1049..1089
                     /label=BBa_B0012
     terminator      1049..1089
                     /label=BBa_B0012
     misc_feature    229..1089
                     /label=BBa_I13507
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagagaa agaggagaaa
      241 tactagatgg cttcctccga agacgttatc aaagagttca tgcgtttcaa agttcgtatg
      301 gaaggttccg ttaacggtca cgagttcgaa atcgaaggtg aaggtgaagg tcgtccgtac
      361 gaaggtaccc agaccgctaa actgaaagtt accaaaggtg gtccgctgcc gttcgcttgg
      421 gacatcctgt ccccgcagtt ccagtacggt tccaaagctt acgttaaaca cccggctgac
      481 atcccggact acctgaaact gtccttcccg gaaggtttca aatgggaacg tgttatgaac
      541 ttcgaagacg gtggtgttgt taccgttacc caggactcct ccctgcaaga cggtgagttc
      601 atctacaaag ttaaactgcg tggtaccaac ttcccgtccg acggtccggt tatgcagaaa
      661 aaaaccatgg gttgggaagc ttccaccgaa cgtatgtacc cggaagacgg tgctctgaaa
      721 ggtgaaatca aaatgcgtct gaaactgaaa gacggtggtc actacgacgc tgaagttaaa
      781 accacctaca tggctaaaaa accggttcag ctgccgggtg cttacaaaac cgacatcaaa
      841 ctggacatca cctcccacaa cgaagactac accatcgttg aacagtacga acgtgctgaa
      901 ggtcgtcact ccaccggtgc ttaataacgc tgatagtgct agtgtagatc gctactagag
      961 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     1021 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
     1081 gcgtttata
//