LOCUS BBa_J147000 2712 bp DNA linear 24-Dec-2025 DEFINITION RFP inducible device regulated by LuxR and C6-HSL through the Lux promoter ACCESSION BBa_J147000 VERSION BBa_J147000.1 FEATURES Location/Qualifiers stem_loop 8..27 /label=T7 TE misc_feature 34..34 /label=stop polyA_site 28..41 /label=polya misc_feature 1..41 /label=BBa_B0012 terminator 1..41 /label=BBa_B0012 stem_loop 62..84 /label=stem_loop misc_feature 50..95 /label=BBa_B0011 terminator 50..95 /label=BBa_B0011 terminator 1..95 /label=BBa_B0014 misc_feature 104..123 /label=LuxR/HSL promoter 123..128 /label=-35 promoter 145..150 /label=-10 misc_feature 156..156 /label=start misc_feature 104..158 /label=BBa_R0062 promoter 104..158 /label=BBa_R0062 RBS 167..179 /label=RBS-3\Weak RBS 173..176 /label=RBS misc_feature 167..179 /label=BBa_B0032 RBS 167..179 /label=BBa_B0032 CDS 186..860 /label=mrfp1 misc_feature 867..891 /label=Help:Barcodes CDS 186..891 /label=BBa_E1010 stem_loop 907..926 /label=T7 TE misc_feature 933..933 /label=stop polyA_site 927..940 /label=polya misc_feature 900..940 /label=BBa_B0012 terminator 900..940 /label=BBa_B0012 stem_loop 961..983 /label=stem_loop misc_feature 949..994 /label=BBa_B0011 terminator 949..994 /label=BBa_B0011 terminator 900..994 /label=BBa_B0014 misc_feature 1003..1021 /label=TetR 1 misc_feature 1003..1056 /label=BBa_R0040 promoter 1022..1027 /label=-35 misc_feature 1028..1046 /label=TetR 2 promoter 1045..1050 /label=-10 promoter 1003..1056 /label=BBa_R0040 misc_feature 1069..1072 /label=conserved RBS 1065..1076 /label=BBa_B0034 misc_feature 1083..1084 /label=prefix misc_feature 1256..1256 /label=T misc_feature 1574..1574 /label=A CDS 1083..1832 /label=luxR misc_feature 1083..1838 /label=BBa_C0062 misc_feature 1839..1863 /label=Help:Barcodes CDS 1083..1838 /label=BBa_C0062 misc_feature 1876..1879 /label=conserved RBS 1872..1883 /label=BBa_B0034 CDS 1890..2609 /label=GFP protein CDS 1890..2609 /label=BBa_E0040 stem_loop 2625..2644 /label=T7 TE misc_feature 2651..2651 /label=stop polyA_site 2645..2658 /label=polya misc_feature 2618..2658 /label=BBa_B0012 terminator 2618..2658 /label=BBa_B0012 stem_loop 2679..2701 /label=stem_loop misc_feature 2667..2712 /label=BBa_B0011 terminator 2667..2712 /label=BBa_B0011 terminator 2618..2712 /label=BBa_B0014 ORIGIN 1 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagaga gagaatataa 61 aaagccagat tattaatccg gcttttttat tattttacta gagacctgta ggatcgtaca 121 ggtttacgca agaaaatggt ttgttatagt cgaataaata ctagagtcac acaggaaagt 181 actagatggc ttcctccgaa gacgttatca aagagttcat gcgtttcaaa gttcgtatgg 241 aaggttccgt taacggtcac gagttcgaaa tcgaaggtga aggtgaaggt cgtccgtacg 301 aaggtaccca gaccgctaaa ctgaaagtta ccaaaggtgg tccgctgccg ttcgcttggg 361 acatcctgtc cccgcagttc cagtacggtt ccaaagctta cgttaaacac ccggctgaca 421 tcccggacta cctgaaactg tccttcccgg aaggtttcaa atgggaacgt gttatgaact 481 tcgaagacgg tggtgttgtt accgttaccc aggactcctc cctgcaagac ggtgagttca 541 tctacaaagt taaactgcgt ggtaccaact tcccgtccga cggtccggtt atgcagaaaa 601 aaaccatggg ttgggaagct tccaccgaac gtatgtaccc ggaagacggt gctctgaaag 661 gtgaaatcaa aatgcgtctg aaactgaaag acggtggtca ctacgacgct gaagttaaaa 721 ccacctacat ggctaaaaaa ccggttcagc tgccgggtgc ttacaaaacc gacatcaaac 781 tggacatcac ctcccacaac gaagactaca ccatcgttga acagtacgaa cgtgctgaag 841 gtcgtcactc caccggtgct taataacgct gatagtgcta gtgtagatcg ctactagagt 901 cacactggct caccttcggg tgggcctttc tgcgtttata tactagagag agaatataaa 961 aagccagatt attaatccgg cttttttatt attttactag agtccctatc agtgatagag 1021 attgacatcc ctatcagtga tagagatact gagcactact agagaaagag gagaaatact 1081 agatgaaaaa cataaatgcc gacgacacat acagaataat taataaaatt aaagcttgta 1141 gaagcaataa tgatattaat caatgcttat ctgatatgac taaaatggta cattgtgaat 1201 attatttact cgcgatcatt tatcctcatt ctatggttaa atctgatatt tcaatcctag 1261 ataattaccc taaaaaatgg aggcaatatt atgatgacgc taatttaata aaatatgatc 1321 ctatagtaga ttattctaac tccaatcatt caccaattaa ttggaatata tttgaaaaca 1381 atgctgtaaa taaaaaatct ccaaatgtaa ttaaagaagc gaaaacatca ggtcttatca 1441 ctgggtttag tttccctatt catacggcta acaatggctt cggaatgctt agttttgcac 1501 attcagaaaa agacaactat atagatagtt tatttttaca tgcgtgtatg aacataccat 1561 taattgttcc ttctctagtt gataattatc gaaaaataaa tatagcaaat aataaatcaa 1621 acaacgattt aaccaaaaga gaaaaagaat gtttagcgtg ggcatgcgaa ggaaaaagct 1681 cttgggatat ttcaaaaata ttaggttgca gtgagcgtac tgtcactttc catttaacca 1741 atgcgcaaat gaaactcaat acaacaaacc gctgccaaag tatttctaaa gcaattttaa 1801 caggagcaat tgattgccca tactttaaaa attaataaca ctgatagtgc tagtgtagat 1861 cactactaga gaaagaggag aaatactaga tgcgtaaagg agaagaactt ttcactggag 1921 ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg 1981 gagagggtga aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg 2041 gaaaactacc tgttccatgg ccaacacttg tcactacttt cggttatggt gttcaatgct 2101 ttgcgagata cccagatcat atgaaacagc atgacttttt caagagtgcc atgcccgaag 2161 gttatgtaca ggaaagaact atatttttca aagatgacgg gaactacaag acacgtgctg 2221 aagtcaagtt tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgatttta 2281 aagaagatgg aaacattctt ggacacaaat tggaatacaa ctataactca cacaatgtat 2341 acatcatggc agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt agacacaaca 2401 ttgaagatgg aagcgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg 2461 gccctgtcct tttaccagac aaccattacc tgtccacaca atctgccctt tcgaaagatc 2521 ccaacgaaaa gagagaccac atggtccttc ttgagtttgt aacagctgct gggattacac 2581 atggcatgga tgaactatac aaataataat actagagtca cactggctca ccttcgggtg 2641 ggcctttctg cgtttatata ctagagagag aatataaaaa gccagattat taatccggct 2701 tttttattat tt //