##gff-version 3
##sequence-region BBa_J147000 1 2712
BBa_J147000	.	terminator	1	95	.	+	0	ID=BBa_B0014;Name=BBa_B0014
BBa_J147000	.	terminator	1	41	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0014
BBa_J147000	.	stop_codon	34	34	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J147000	.	polyA_site	28	41	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J147000	.	stem_loop	8	27	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J147000	.	engineered_region	1	41	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J147000	.	terminator	50	95	.	+	0	ID=BBa_B0011;Name=BBa_B0011;Parent=BBa_B0014
BBa_J147000	.	engineered_region	50	95	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_J147000	.	stem_loop	62	84	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_J147000	.	terminator	2618	2712	.	+	0	ID=BBa_B0014;Name=BBa_B0014
BBa_J147000	.	terminator	2618	2658	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0014
BBa_J147000	.	stop_codon	2651	2651	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J147000	.	polyA_site	2645	2658	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J147000	.	stem_loop	2625	2644	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J147000	.	engineered_region	2618	2658	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J147000	.	terminator	2667	2712	.	+	0	ID=BBa_B0011;Name=BBa_B0011;Parent=BBa_B0014
BBa_J147000	.	engineered_region	2667	2712	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_J147000	.	stem_loop	2679	2701	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_J147000	.	CDS	1083	1838	.	+	0	ID=BBa_C0062;Name=BBa_C0062
BBa_J147000	.	engineered_tag	1839	1863	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_J147000	.	engineered_region	1083	1838	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_J147000	.	engineered_region	1083	1084	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_J147000	.	point_mutation	1256	1256	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_J147000	.	CDS	1083	1832	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_J147000	.	point_mutation	1574	1574	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_J147000	.	ribosome_entry_site	167	179	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_J147000	.	ribosome_entry_site	167	179	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_J147000	.	engineered_region	167	179	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_J147000	.	ribosome_entry_site	173	176	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_J147000	.	CDS	186	891	.	+	0	ID=BBa_E1010;Name=BBa_E1010
BBa_J147000	.	engineered_tag	867	891	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_J147000	.	CDS	186	860	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_J147000	.	promoter	104	158	.	+	0	ID=BBa_R0062;Name=BBa_R0062
BBa_J147000	.	start_codon	156	156	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_J147000	.	promoter	145	150	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_J147000	.	promoter	123	128	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_J147000	.	engineered_region	104	158	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_J147000	.	non_covalent_binding_site	104	123	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_J147000	.	ribosome_entry_site	1872	1883	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J147000	.	conserved_region	1876	1879	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J147000	.	terminator	900	994	.	+	0	ID=BBa_B0014;Name=BBa_B0014
BBa_J147000	.	terminator	900	940	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0014
BBa_J147000	.	stop_codon	933	933	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J147000	.	polyA_site	927	940	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J147000	.	stem_loop	907	926	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J147000	.	engineered_region	900	940	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J147000	.	terminator	949	994	.	+	0	ID=BBa_B0011;Name=BBa_B0011;Parent=BBa_B0014
BBa_J147000	.	engineered_region	949	994	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_J147000	.	stem_loop	961	983	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_J147000	.	ribosome_entry_site	1065	1076	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J147000	.	conserved_region	1069	1072	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J147000	.	promoter	1003	1056	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_J147000	.	engineered_region	1003	1056	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J147000	.	promoter	1022	1027	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J147000	.	promoter	1045	1050	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J147000	.	non_covalent_binding_site	1003	1021	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J147000	.	non_covalent_binding_site	1028	1046	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J147000	.	CDS	1890	2609	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_J147000	.	CDS	1890	2609	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
>BBa_J147000
tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatcc
ggcttttttattattttactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaa
tactagagtcacacaggaaagtactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgta
tggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgc
taaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaa
gcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgtta
tgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaa
actgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgt
atgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctg
aagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcac
ctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgc
tgatagtgctagtgtagatcgctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagag
agagaatataaaaagccagattattaatccggcttttttattattttactagagtccctatcagtgatagagattgaca
tccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgaaaaacataaatgccgacgac
acatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaa
tggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataa
ttaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactcc
aatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcga
aaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcaca
ttcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagtt
gataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgt
gggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaac
caatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgc
ccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagaaagaggagaaatactagatgcgta
aaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgt
cagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgtt
ccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatg
actttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagac
acgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatgga
aacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaa
tcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactcc
aattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaa
aagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataat
aatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccaga
ttattaatccggcttttttattattt