LOCUS BBa_J147001 2714 bp DNA linear 12-Mar-2025 DEFINITION RFP inducible device regulated by LuxR and C6-HSL through the Lux promoter ACCESSION BBa_J147001 VERSION BBa_J147001.1 FEATURES Location/Qualifiers stem_loop 8..27 /label=T7 TE misc_feature 34..34 /label=stop polyA_site 28..41 /label=polya misc_feature 1..41 /label=BBa_B0012 terminator 1..41 /label=BBa_B0012 stem_loop 62..84 /label=stem_loop misc_feature 50..95 /label=BBa_B0011 terminator 50..95 /label=BBa_B0011 terminator 1..95 /label=BBa_B0014 misc_feature 104..123 /label=LuxR/HSL promoter 123..128 /label=-35 promoter 145..150 /label=-10 misc_feature 156..156 /label=start misc_feature 104..158 /label=BBa_R0062 promoter 104..158 /label=BBa_R0062 RBS 167..179 /label=RBS-3\Weak RBS 173..176 /label=RBS misc_feature 167..179 /label=BBa_B0032 RBS 167..179 /label=BBa_B0032 CDS 186..860 /label=mrfp1 misc_feature 867..891 /label=Help:Barcodes CDS 186..891 /label=BBa_E1010 stem_loop 907..926 /label=T7 TE misc_feature 933..933 /label=stop polyA_site 927..940 /label=polya misc_feature 900..940 /label=BBa_B0012 terminator 900..940 /label=BBa_B0012 stem_loop 961..983 /label=stem_loop misc_feature 949..994 /label=BBa_B0011 terminator 949..994 /label=BBa_B0011 terminator 900..994 /label=BBa_B0014 misc_feature 1003..1021 /label=TetR 1 misc_feature 1003..1056 /label=BBa_R0040 promoter 1022..1027 /label=-35 misc_feature 1028..1046 /label=TetR 2 promoter 1045..1050 /label=-10 promoter 1003..1056 /label=BBa_R0040 RBS 1065..1077 /label=RBS-3\Weak RBS 1071..1074 /label=RBS misc_feature 1065..1077 /label=BBa_B0032 RBS 1065..1077 /label=BBa_B0032 misc_feature 1084..1085 /label=prefix misc_feature 1257..1257 /label=T misc_feature 1575..1575 /label=A CDS 1084..1833 /label=luxR misc_feature 1084..1839 /label=BBa_C0062 misc_feature 1840..1864 /label=Help:Barcodes CDS 1084..1839 /label=BBa_C0062 RBS 1873..1885 /label=RBS-3\Weak RBS 1879..1882 /label=RBS misc_feature 1873..1885 /label=BBa_B0032 RBS 1873..1885 /label=BBa_B0032 CDS 1892..2611 /label=GFP protein CDS 1892..2611 /label=BBa_E0040 stem_loop 2627..2646 /label=T7 TE misc_feature 2653..2653 /label=stop polyA_site 2647..2660 /label=polya misc_feature 2620..2660 /label=BBa_B0012 terminator 2620..2660 /label=BBa_B0012 stem_loop 2681..2703 /label=stem_loop misc_feature 2669..2714 /label=BBa_B0011 terminator 2669..2714 /label=BBa_B0011 terminator 2620..2714 /label=BBa_B0014 ORIGIN 1 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagaga gagaatataa 61 aaagccagat tattaatccg gcttttttat tattttacta gagacctgta ggatcgtaca 121 ggtttacgca agaaaatggt ttgttatagt cgaataaata ctagagtcac acaggaaagt 181 actagatggc ttcctccgaa gacgttatca aagagttcat gcgtttcaaa gttcgtatgg 241 aaggttccgt taacggtcac gagttcgaaa tcgaaggtga aggtgaaggt cgtccgtacg 301 aaggtaccca gaccgctaaa ctgaaagtta ccaaaggtgg tccgctgccg ttcgcttggg 361 acatcctgtc cccgcagttc cagtacggtt ccaaagctta cgttaaacac ccggctgaca 421 tcccggacta cctgaaactg tccttcccgg aaggtttcaa atgggaacgt gttatgaact 481 tcgaagacgg tggtgttgtt accgttaccc aggactcctc cctgcaagac ggtgagttca 541 tctacaaagt taaactgcgt ggtaccaact tcccgtccga cggtccggtt atgcagaaaa 601 aaaccatggg ttgggaagct tccaccgaac gtatgtaccc ggaagacggt gctctgaaag 661 gtgaaatcaa aatgcgtctg aaactgaaag acggtggtca ctacgacgct gaagttaaaa 721 ccacctacat ggctaaaaaa ccggttcagc tgccgggtgc ttacaaaacc gacatcaaac 781 tggacatcac ctcccacaac gaagactaca ccatcgttga acagtacgaa cgtgctgaag 841 gtcgtcactc caccggtgct taataacgct gatagtgcta gtgtagatcg ctactagagt 901 cacactggct caccttcggg tgggcctttc tgcgtttata tactagagag agaatataaa 961 aagccagatt attaatccgg cttttttatt attttactag agtccctatc agtgatagag 1021 attgacatcc ctatcagtga tagagatact gagcactact agagtcacac aggaaagtac 1081 tagatgaaaa acataaatgc cgacgacaca tacagaataa ttaataaaat taaagcttgt 1141 agaagcaata atgatattaa tcaatgctta tctgatatga ctaaaatggt acattgtgaa 1201 tattatttac tcgcgatcat ttatcctcat tctatggtta aatctgatat ttcaatccta 1261 gataattacc ctaaaaaatg gaggcaatat tatgatgacg ctaatttaat aaaatatgat 1321 cctatagtag attattctaa ctccaatcat tcaccaatta attggaatat atttgaaaac 1381 aatgctgtaa ataaaaaatc tccaaatgta attaaagaag cgaaaacatc aggtcttatc 1441 actgggttta gtttccctat tcatacggct aacaatggct tcggaatgct tagttttgca 1501 cattcagaaa aagacaacta tatagatagt ttatttttac atgcgtgtat gaacatacca 1561 ttaattgttc cttctctagt tgataattat cgaaaaataa atatagcaaa taataaatca 1621 aacaacgatt taaccaaaag agaaaaagaa tgtttagcgt gggcatgcga aggaaaaagc 1681 tcttgggata tttcaaaaat attaggttgc agtgagcgta ctgtcacttt ccatttaacc 1741 aatgcgcaaa tgaaactcaa tacaacaaac cgctgccaaa gtatttctaa agcaatttta 1801 acaggagcaa ttgattgccc atactttaaa aattaataac actgatagtg ctagtgtaga 1861 tcactactag agtcacacag gaaagtacta gatgcgtaaa ggagaagaac ttttcactgg 1921 agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag 1981 tggagagggt gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac 2041 tggaaaacta cctgttccat ggccaacact tgtcactact ttcggttatg gtgttcaatg 2101 ctttgcgaga tacccagatc atatgaaaca gcatgacttt ttcaagagtg ccatgcccga 2161 aggttatgta caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtgc 2221 tgaagtcaag tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt 2281 taaagaagat ggaaacattc ttggacacaa attggaatac aactataact cacacaatgt 2341 atacatcatg gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa 2401 cattgaagat ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga 2461 tggccctgtc cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga 2521 tcccaacgaa aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac 2581 acatggcatg gatgaactat acaaataata atactagagt cacactggct caccttcggg 2641 tgggcctttc tgcgtttata tactagagag agaatataaa aagccagatt attaatccgg 2701 cttttttatt attt //