##gff-version 3
##sequence-region BBa_J147001 1 2714
BBa_J147001	.	ribosome_entry_site	1873	1885	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_J147001	.	engineered_region	1873	1885	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_J147001	.	ribosome_entry_site	1873	1885	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_J147001	.	ribosome_entry_site	1879	1882	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_J147001	.	terminator	1	95	.	+	0	ID=BBa_B0014;Name=BBa_B0014
BBa_J147001	.	terminator	50	95	.	+	0	ID=BBa_B0011;Name=BBa_B0011;Parent=BBa_B0014
BBa_J147001	.	stem_loop	62	84	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_J147001	.	engineered_region	50	95	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_J147001	.	terminator	1	41	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0014
BBa_J147001	.	stop_codon	34	34	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J147001	.	stem_loop	8	27	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J147001	.	polyA_site	28	41	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J147001	.	engineered_region	1	41	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J147001	.	promoter	1003	1056	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_J147001	.	promoter	1022	1027	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J147001	.	non_covalent_binding_site	1028	1046	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J147001	.	promoter	1045	1050	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J147001	.	non_covalent_binding_site	1003	1021	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J147001	.	engineered_region	1003	1056	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J147001	.	ribosome_entry_site	167	179	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_J147001	.	engineered_region	167	179	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_J147001	.	ribosome_entry_site	167	179	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_J147001	.	ribosome_entry_site	173	176	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_J147001	.	terminator	2620	2714	.	+	0	ID=BBa_B0014;Name=BBa_B0014
BBa_J147001	.	terminator	2669	2714	.	+	0	ID=BBa_B0011;Name=BBa_B0011;Parent=BBa_B0014
BBa_J147001	.	stem_loop	2681	2703	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_J147001	.	engineered_region	2669	2714	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_J147001	.	terminator	2620	2660	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0014
BBa_J147001	.	stop_codon	2653	2653	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J147001	.	stem_loop	2627	2646	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J147001	.	polyA_site	2647	2660	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J147001	.	engineered_region	2620	2660	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J147001	.	CDS	1892	2611	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_J147001	.	CDS	1892	2611	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_J147001	.	ribosome_entry_site	1065	1077	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_J147001	.	engineered_region	1065	1077	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_J147001	.	ribosome_entry_site	1065	1077	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_J147001	.	ribosome_entry_site	1071	1074	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_J147001	.	CDS	186	891	.	+	0	ID=BBa_E1010;Name=BBa_E1010
BBa_J147001	.	engineered_tag	867	891	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_J147001	.	CDS	186	860	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_J147001	.	CDS	1084	1839	.	+	0	ID=BBa_C0062;Name=BBa_C0062
BBa_J147001	.	engineered_region	1084	1839	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_J147001	.	CDS	1084	1833	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_J147001	.	engineered_region	1084	1085	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_J147001	.	point_mutation	1257	1257	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_J147001	.	engineered_tag	1840	1864	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_J147001	.	point_mutation	1575	1575	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_J147001	.	promoter	104	158	.	+	0	ID=BBa_R0062;Name=BBa_R0062
BBa_J147001	.	non_covalent_binding_site	104	123	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_J147001	.	start_codon	156	156	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_J147001	.	promoter	123	128	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_J147001	.	promoter	145	150	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_J147001	.	engineered_region	104	158	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_J147001	.	terminator	900	994	.	+	0	ID=BBa_B0014;Name=BBa_B0014
BBa_J147001	.	terminator	949	994	.	+	0	ID=BBa_B0011;Name=BBa_B0011;Parent=BBa_B0014
BBa_J147001	.	stem_loop	961	983	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_J147001	.	engineered_region	949	994	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_J147001	.	terminator	900	940	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0014
BBa_J147001	.	stop_codon	933	933	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J147001	.	stem_loop	907	926	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J147001	.	polyA_site	927	940	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J147001	.	engineered_region	900	940	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
>BBa_J147001
tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatcc
ggcttttttattattttactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaa
tactagagtcacacaggaaagtactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgta
tggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgc
taaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaa
gcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgtta
tgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaa
actgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgt
atgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctg
aagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcac
ctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgc
tgatagtgctagtgtagatcgctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagag
agagaatataaaaagccagattattaatccggcttttttattattttactagagtccctatcagtgatagagattgaca
tccctatcagtgatagagatactgagcactactagagtcacacaggaaagtactagatgaaaaacataaatgccgacga
cacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaa
atggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagata
attaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactc
caatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcg
aaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcac
attcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagt
tgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcg
tgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaa
ccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattg
cccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagtcacacaggaaagtactagatgcg
taaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttct
gtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctg
ttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagca
tgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaag
acacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatg
gaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatgg
aatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatact
ccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacg
aaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaata
ataatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagcca
gattattaatccggcttttttattattt