LOCUS BBa_J147002 2710 bp DNA linear 23-Dec-2024 DEFINITION RFP inducible device regulated by LuxR and C6-HSL through the Lux promoter ACCESSION BBa_J147002 VERSION BBa_J147002.1 FEATURES Location/Qualifiers stem_loop 8..27 /label=T7 TE misc_feature 34..34 /label=stop polyA_site 28..41 /label=polya misc_feature 1..41 /label=BBa_B0012 terminator 1..41 /label=BBa_B0012 stem_loop 62..84 /label=stem_loop misc_feature 50..95 /label=BBa_B0011 terminator 50..95 /label=BBa_B0011 terminator 1..95 /label=BBa_B0014 misc_feature 104..123 /label=LuxR/HSL promoter 123..128 /label=-35 promoter 145..150 /label=-10 misc_feature 156..156 /label=start misc_feature 104..158 /label=BBa_R0062 promoter 104..158 /label=BBa_R0062 RBS 167..179 /label=RBS-3\Weak RBS 173..176 /label=RBS misc_feature 167..179 /label=BBa_B0032 RBS 167..179 /label=BBa_B0032 CDS 186..860 /label=mrfp1 misc_feature 867..891 /label=Help:Barcodes CDS 186..891 /label=BBa_E1010 stem_loop 907..926 /label=T7 TE misc_feature 933..933 /label=stop polyA_site 927..940 /label=polya misc_feature 900..940 /label=BBa_B0012 terminator 900..940 /label=BBa_B0012 stem_loop 961..983 /label=stem_loop misc_feature 949..994 /label=BBa_B0011 terminator 949..994 /label=BBa_B0011 terminator 900..994 /label=BBa_B0014 misc_feature 1003..1021 /label=TetR 1 misc_feature 1003..1056 /label=BBa_R0040 promoter 1022..1027 /label=-35 misc_feature 1028..1046 /label=TetR 2 promoter 1045..1050 /label=-10 promoter 1003..1056 /label=BBa_R0040 RBS 1065..1075 /label=RBS-4\Weaker RBS 1071..1074 /label=RBS misc_feature 1065..1075 /label=BBa_B0033 RBS 1065..1075 /label=BBa_B0033 misc_feature 1082..1083 /label=prefix misc_feature 1255..1255 /label=T misc_feature 1573..1573 /label=A CDS 1082..1831 /label=luxR misc_feature 1082..1837 /label=BBa_C0062 misc_feature 1838..1862 /label=Help:Barcodes CDS 1082..1837 /label=BBa_C0062 RBS 1871..1881 /label=RBS-4\Weaker RBS 1877..1880 /label=RBS misc_feature 1871..1881 /label=BBa_B0033 RBS 1871..1881 /label=BBa_B0033 CDS 1888..2607 /label=GFP protein CDS 1888..2607 /label=BBa_E0040 stem_loop 2623..2642 /label=T7 TE misc_feature 2649..2649 /label=stop polyA_site 2643..2656 /label=polya misc_feature 2616..2656 /label=BBa_B0012 terminator 2616..2656 /label=BBa_B0012 stem_loop 2677..2699 /label=stem_loop misc_feature 2665..2710 /label=BBa_B0011 terminator 2665..2710 /label=BBa_B0011 terminator 2616..2710 /label=BBa_B0014 ORIGIN 1 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagaga gagaatataa 61 aaagccagat tattaatccg gcttttttat tattttacta gagacctgta ggatcgtaca 121 ggtttacgca agaaaatggt ttgttatagt cgaataaata ctagagtcac acaggaaagt 181 actagatggc ttcctccgaa gacgttatca aagagttcat gcgtttcaaa gttcgtatgg 241 aaggttccgt taacggtcac gagttcgaaa tcgaaggtga aggtgaaggt cgtccgtacg 301 aaggtaccca gaccgctaaa ctgaaagtta ccaaaggtgg tccgctgccg ttcgcttggg 361 acatcctgtc cccgcagttc cagtacggtt ccaaagctta cgttaaacac ccggctgaca 421 tcccggacta cctgaaactg tccttcccgg aaggtttcaa atgggaacgt gttatgaact 481 tcgaagacgg tggtgttgtt accgttaccc aggactcctc cctgcaagac ggtgagttca 541 tctacaaagt taaactgcgt ggtaccaact tcccgtccga cggtccggtt atgcagaaaa 601 aaaccatggg ttgggaagct tccaccgaac gtatgtaccc ggaagacggt gctctgaaag 661 gtgaaatcaa aatgcgtctg aaactgaaag acggtggtca ctacgacgct gaagttaaaa 721 ccacctacat ggctaaaaaa ccggttcagc tgccgggtgc ttacaaaacc gacatcaaac 781 tggacatcac ctcccacaac gaagactaca ccatcgttga acagtacgaa cgtgctgaag 841 gtcgtcactc caccggtgct taataacgct gatagtgcta gtgtagatcg ctactagagt 901 cacactggct caccttcggg tgggcctttc tgcgtttata tactagagag agaatataaa 961 aagccagatt attaatccgg cttttttatt attttactag agtccctatc agtgatagag 1021 attgacatcc ctatcagtga tagagatact gagcactact agagtcacac aggactacta 1081 gatgaaaaac ataaatgccg acgacacata cagaataatt aataaaatta aagcttgtag 1141 aagcaataat gatattaatc aatgcttatc tgatatgact aaaatggtac attgtgaata 1201 ttatttactc gcgatcattt atcctcattc tatggttaaa tctgatattt caatcctaga 1261 taattaccct aaaaaatgga ggcaatatta tgatgacgct aatttaataa aatatgatcc 1321 tatagtagat tattctaact ccaatcattc accaattaat tggaatatat ttgaaaacaa 1381 tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg aaaacatcag gtcttatcac 1441 tgggtttagt ttccctattc atacggctaa caatggcttc ggaatgctta gttttgcaca 1501 ttcagaaaaa gacaactata tagatagttt atttttacat gcgtgtatga acataccatt 1561 aattgttcct tctctagttg ataattatcg aaaaataaat atagcaaata ataaatcaaa 1621 caacgattta accaaaagag aaaaagaatg tttagcgtgg gcatgcgaag gaaaaagctc 1681 ttgggatatt tcaaaaatat taggttgcag tgagcgtact gtcactttcc atttaaccaa 1741 tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt atttctaaag caattttaac 1801 aggagcaatt gattgcccat actttaaaaa ttaataacac tgatagtgct agtgtagatc 1861 actactagag tcacacagga ctactagatg cgtaaaggag aagaactttt cactggagtt 1921 gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga 1981 gagggtgaag gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga 2041 aaactacctg ttccatggcc aacacttgtc actactttcg gttatggtgt tcaatgcttt 2101 gcgagatacc cagatcatat gaaacagcat gactttttca agagtgccat gcccgaaggt 2161 tatgtacagg aaagaactat atttttcaaa gatgacggga actacaagac acgtgctgaa 2221 gtcaagtttg aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa 2281 gaagatggaa acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac 2341 atcatggcag acaaacaaaa gaatggaatc aaagttaact tcaaaattag acacaacatt 2401 gaagatggaa gcgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc 2461 cctgtccttt taccagacaa ccattacctg tccacacaat ctgccctttc gaaagatccc 2521 aacgaaaaga gagaccacat ggtccttctt gagtttgtaa cagctgctgg gattacacat 2581 ggcatggatg aactatacaa ataataatac tagagtcaca ctggctcacc ttcgggtggg 2641 cctttctgcg tttatatact agagagagaa tataaaaagc cagattatta atccggcttt 2701 tttattattt //