BBa_J15102
1
BBa_J15102
lacZ' coding sequence
2007-07-12T11:00:00Z
2015-08-31T04:08:32Z
Escherichia coli BS. The sequence was obtained from gi:146575.
Released HQ 2013
Escherichia coli lacZ' coding sequence encoding the first 76 amino acids of LacZ (beta-galactosidase). This is sufficient to complement the lacZ-delta-M15 mutation found in commonly used laboratory strains of E. coli, thus allowing blue-white selection. It also can be used to effect a pH drop in the presence of lactose, as in the arsenic biosensor entered by the University of Ediburgh team for iGEM2006.
false
false
_163_
0
837
163
In stock
false
Codon 77, TGC, was changed to TAA to truncate the coding sequence.
false
Chris French
annotation1938053
1
end lacZ
range1938053
1
229
231
annotation1938051
1
lacZ'
range1938051
1
1
231
annotation1938052
1
start lacZ'
range1938052
1
1
3
BBa_J15102_sequence
1
atgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z