BBa_J15103
1
BBa_J15103
lacZ' coding sequence ending in TGA
2007-07-12T11:00:00Z
2015-08-31T04:08:32Z
Eschericia coli BS. The sequence was derived from gi:146575.
This is the same Escherichia coli lacZ' coding sequence as in BBa_J15102, encoding the N-terminal 76 amino acids of LacZ (beta galactosidase), except that it ends with TGA rather than the recommended TAATAA. It was mistakenly incorporated into several later constructs where we had intended to use BBa_J15012. It works exactly the same, but does not fully conform to the Registry recommendations for coding sequences.
false
false
_163_
0
837
163
Not in stock
false
Codon 77, normally TGC, was altered to TGA to truncate the sequence.
false
Chris French
annotation1938055
1
start lacZ'
range1938055
1
1
3
annotation1938054
1
lacZ'
range1938054
1
1
231
annotation1938056
1
end lacZ'
range1938056
1
229
231
annotation1938057
1
silent mutation codon 58
range1938057
1
174
174
BBa_J15103_sequence
1
atgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgagtggcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z