LOCUS BBa_J153000 7697 bp DNA linear 22-Dec-2024 DEFINITION Broad-host-range shuttle vector pPMQAK1 ACCESSION BBa_J153000 VERSION BBa_J153000.1 FEATURES Location/Qualifiers misc_feature 1..21 /label=Suffix stem_loop 30..62 /label=Terminator primer_bind 155..174 /label=VR (BBa_G00101) misc_feature 265..655 /label=oriV CDS 682..966 /label=mobC CDS 1165..3294 /label=mobA CDS 1913..2326 /label=mobB CDS 2323..3294 /label=repB misc_feature 3355..3567 /label=unknown protein E (predicted) misc_feature 3569..3775 /label=repressor protein F (predicted) CDS 3805..4644 /label=repA CDS 4631..5482 /label=repC CDS 5605..6420 /label=kanR CDS 6575..7435 /label=ampR primer_bind 7557..7577 /label=VF2 (BBa_G00100) stem_loop 7647..7666 /label=Terminator misc_feature 7676..7697 /label=Prefix ORIGIN 1 tactagtagc ggccgctgca gtccggcaaa aaagggcaag gtgtcaccac cctgcccttt 61 ttctttaaaa ccgaaaagat tacttcgcgt tatgcaggct tcctcgctca ctgactcgct 121 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatcaattg 181 agttctttta ccctcagccg aaatgcctgc cgttgctaga cattgccagc cagtgcccgt 241 cactcccgta ctaactgtca cgaacccctg caataactgt cacgcccccc tgcaataact 301 gtcacgaacc cctgcaataa ctgtcacgcc cccaaacctg caaacccagc aggggcgggg 361 gctggcgggg tgttggaaaa atccatccat gattatctaa gaataatcca ctaggcgcgg 421 ttatcagcgc ccttgtgggg cgctgctgcc cttgcccaat atgcccggcc agaggccgga 481 tagctggtct attcgctgcg ctaggctaca caccgcccca ccgctgcgcg gcagggggaa 541 aggcgggcaa agcccgctaa accccacacc aaaccccgca gaaatacgct ggagcgcttt 601 tagccgcttt agcggccttt ccccctaccc gaagggtggg ggcgcgtgtg cagccccgca 661 gggcctgtct cggtcgatca ttcagcccgg ctcatccttc tggcgtggcg gcagaccgaa 721 caaggcgcgg tcgtggtcgc gttcaaggta cgcatccatt gccgccatga gccgatcctc 781 cggccactcg ctgctgttca ccttggccaa aatcatggcc cccaccagca ccttgcgcct 841 tgtttcgttc ttgcgctctt gctgctgttc ccttgcccgc acccgctgaa tttcggcatt 901 gattcgcgct cgttgttctt cgagcttggc cagccgatcc gccgccttgt tgctcccctt 961 aaccatcttg acaccccatt gttaatgtgc tgtctcgtag gctatcatgg aggcacagcg 1021 gcggcaatcc cgaccctact ttgtagggga gggcgcactt accggtttct cttcgagaaa 1081 ctggcctaac ggccaccctt cgggcggtgc gctctccgag ggccattgca tggagccgaa 1141 aagcaaaagc aacagcgagg cagcatggcg atttatcacc ttacggcgaa aaccggcagc 1201 aggtcgggcg gccaatcggc cagggccaag gccgactaca tccagcgcga aggcaagtat 1261 gcccgcgaca tggatgaagt cttgcacgcc gaatccgggc acatgccgga gttcgtcgag 1321 cggcccgccg actactggga tgctgccgac ctgtatgaac gcgccaatgg gcggctgttc 1381 aaggaggtcg aatttgccct gccggtcgag ctgaccctcg accagcagaa ggcgctggcg 1441 tccgagttcg cccagcacct gaccggtgcc gagcgcctgc cgtatacgct ggccatccat 1501 gccggtggcg gcgagaaccc gcactgccac ctgatgatct ccgagcggat caatgacggc 1561 atcgagcggc ccgccgctca gtggttcaag cggtacaacg gcaagacccc ggagaagggc 1621 ggggcacaga agaccgaagc gctcaagccc aaggcatggc ttgagcagac ccgcgaggca 1681 tgggccgacc atgccaaccg ggcattagag cgggctggcc acgacgcccg cattgaccac 1741 agaacacttg aggcgcaggg catcgagcgc ctgcccggtg ttcacctggg gccgaacgtg 1801 gtggagatgg aaggccgggg catccgcacc gaccgggcag acgtggccct gaacatcgac 1861 accgccaacg cccagatcat cgacttacag gaataccggg aggcaataga ccatgaacgc 1921 aatcgacaga gtgaagaaat ccagaggcat caacgagtta gcggagcaga tcgaaccgct 1981 ggcccagagc atggcgacac tggccgacga agcccggcag gtcatgagcc agacccagca 2041 ggccagcgag gcgcaggcgg cggagtggct gaaagcccag cgccagacag gggcggcatg 2101 ggtggagctg gccaaagagt tgcgggaggt agccgccgag gtgagcagcg ccgcgcagag 2161 cgcccggagc gcgtcgcggg ggtggcactg gaagctatgg ctaaccgtga tgctggcttc 2221 catgatgcct acggtggtgc tgctgatcgc atcgttgctc ttgctcgacc tgacgccact 2281 gacaaccgag gacggctcga tctggctgcg cttggtggcc cgatgaagaa cgacaggact 2341 ttgcaggcca taggccgaca gctcaaggcc atgggctgtg agcgcttcga tatcggcgtc 2401 agggacgcca ccaccggcca gatgatgaac cgggaatggt cagccgccga agtgctccag 2461 aacacgccat ggctcaagcg gatgaatgcc cagggcaatg acgtgtatat caggcccgcc 2521 gagcaggagc ggcatggtct ggtgctggtg gacgacctca gcgagtttga cctggatgac 2581 atgaaagccg agggccggga gcctgccctg gtagtggaaa ccagcccgaa gaactatcag 2641 gcatgggtca aggtggccga cgccgcaggc ggtgaacttc gggggcagat tgcccggacg 2701 ctggccagcg agtacgacgc cgacccggcc agcgccgaca gccgccacta tggccgcttg 2761 gcgggcttca ccaaccgcaa ggacaagcac accacccgcg ccggttatca gccgtgggtg 2821 ctgctgcgtg aatccaaggg caagaccgcc accgctggcc cggcgctggt gcagcaggct 2881 ggccagcaga tcgagcaggc ccagcggcag caggagaagg cccgcaggct ggccagcctc 2941 gaactgcccg agcggcagct tagccgccac cggcgcacgg cgctggacga gtaccgcagc 3001 gagatggccg ggctggtcaa gcgcttcggt gatgacctca gcaagtgcga ctttatcgcc 3061 gcgcagaagc tggccagccg gggccgcagt gccgaggaaa tcggcaaggc catggccgag 3121 gccagcccag cgctggcaga gcgcaagccc ggccacgaag cggattacat cgagcgcacc 3181 gtcagcaagg tcatgggtct gcccagcgtc cagcttgcgc gggccgagct ggcacgggca 3241 ccggcacccc gccagcgagg catggacagg ggcgggccag atttcagcat gtagtgcttg 3301 cgttggtact cacgcctgtt atactatgag tactcacgca cagaaggggg ttttatggaa 3361 tacgaaaaaa gcgcttcagg gtcggtctac ctgatcaaaa gtgacaaggg ctattggttg 3421 cccggtggct ttggttatac gtcaaacaag gccgaggctg gccgcttttc agtcgctgat 3481 atggccagcc ttaaccttga cggctgcacc ttgtccttgt tccgcgaaga caagcctttc 3541 ggccccggca agtttctcgg tgactgatat gaaagaccaa aaggacaagc agaccggcga 3601 cctgctggcc agccctgacg ctgtacgcca agcgcgatat gccgagcgca tgaaggccaa 3661 agggatgcgt cagcgcaagt tctggctgac cgacgacgaa tacgaggcgc tgcgcgagtg 3721 cctggaagaa ctcagagcgg cgcagggcgg gggtagtgac cccgccagcg cctaaccacc 3781 aactgcctgc aaaggaggca atcaatggct acccataagc ctatcaatat tctggaggcg 3841 ttcgcagcag cgccgccacc gctggactac gttttgccca acatggtggc cggtacggtc 3901 ggggcgctgg tgtcgcccgg tggtgccggt aaatccatgc tggccctgca actggccgca 3961 cagattgcag gcgggccgga tctgctggag gtgggcgaac tgcccaccgg cccggtgatc 4021 tacctgcccg ccgaagaccc gcccaccgcc attcatcacc gcctgcacgc ccttggggcg 4081 cacctcagcg ccgaggaacg gcaagccgtg gctgacggcc tgctgatcca gccgctgatc 4141 ggcagcctgc ccaacatcat ggccccggag tggttcgacg gcctcaagcg cgccgccgag 4201 ggccgccgcc tgatggtgct ggacacgctg cgccggttcc acatcgagga agaaaacgcc 4261 agcggcccca tggcccaggt catcggtcgc atggaggcca tcgccgccga taccgggtgc 4321 tctatcgtgt tcctgcacca tgccagcaag ggcgcggcca tgatgggcgc aggcgaccag 4381 cagcaggcca gccggggcag ctcggtactg gtcgataaca tccgctggca gtcctacctg 4441 tcgagcatga ccagcgccga ggccgaggaa tggggtgtgg acgacgacca gcgccggttc 4501 ttcgtccgct tcggtgtgag caaggccaac tatggcgcac cgttcgctga tcggtggttc 4561 aggcggcatg acggcggggt gctcaagccc gccgtgctgg agaggcagcg caagagcaag 4621 ggggtgcccc gtggtgaagc ctaagaacaa gcacagcctc agccacgtcc ggcacgaccc 4681 ggcgcactgt ctggcccccg gcctgttccg tgccctcaag cggggcgagc gcaagcgcag 4741 caagctggac gtgacgtatg actacggcga cggcaagcgg atcgagttca gcggcccgga 4801 gccgctgggc gctgatgatc tgcgcatcct gcaagggctg gtggccatgg ctgggcctaa 4861 tggcctagtg cttggcccgg aacccaagac cgaaggcgga cggcagctcc ggctgttcct 4921 ggaacccaag tgggaggccg tcaccgctga atgccatgtg gtcaaaggta gctatcgggc 4981 gctggcaaag gaaatcgggg cagaggtcga tagtggtggg gcgctcaagc acatacagga 5041 ctgcatcgag cgcctttgga aggtatccat catcgcccag aatggccgca agcggcaggg 5101 gtttcggctg ctgtcggagt acgccagcga cgaggcggac gggcgcctgt acgtggccct 5161 gaaccccttg atcgcgcagg ccgtcatggg tggcggccag catgtgcgca tcagcatgga 5221 cgaggtgcgg gcgctggaca gcgaaaccgc ccgcctgctg caccagcggc tgtgtggctg 5281 gatcgacccc ggcaaaaccg gcaaggcttc catagatacc ttgtgcggct atgtctggcc 5341 gtcagaggcc agtggttcga ccatgcgcaa gcgccgccag cgggtgcgcg aggcgttgcc 5401 ggagctggtc gcgctgggct ggacggtaac cgagttcgcg gcgggcaagt acgacatcac 5461 ccggcccaag gcggcaggct gacccccccc actctattgt aaacaagaca tttttatctt 5521 ttatattcaa tggcttattt tcctgctaat tggtaatacc atggtaccgc tctgccagtg 5581 ttacaaccaa ttaaccaatt ctgattagaa aaactcatcg agcatcaaat gaaactgcaa 5641 tttattcata tcaggattat caataccata tttttgaaaa agccgtttct gtaatgaagg 5701 agaaaactca ccgaggcagt tccataggat ggcaagatcc tggtatcggt ctgcgattcc 5761 gactcgtcca acatcaatac aacctattaa tttcccctcg tcaaaaataa ggttatcaag 5821 tgagaaatca ccatgagtga cgactgaatc cggtgagaat ggcaaaagct tatgcatttc 5881 tttccagact tgttcaacag gccagccatt acgctcgtca tcaaaatcac tcgcatcaac 5941 caaaccgtta ttcattcgtg attgcgcctg agcgagacga aatacgcgat cgctgttaaa 6001 aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac 6061 aatattttca cctgaatcag gatattcttc taatacctgg aatgctgttt tcccggggat 6121 cgcagtggtg agtaaccatg catcatcagg agtacggata aaatgcttga tggtcggaag 6181 aggcataaat tccgtcagcc agtttagtct gaccatctca tctgtaacat cattggcaac 6241 gctacctttg ccatgtttca gaaacaactc tggcgcatcg ggcttcccat acaatcgata 6301 gattgtcgca cctgattgcc cgacattatc gcgagcccat ttatacccat ataaatcagc 6361 atccatgttg gaatttaatc gcggcctgga gcaagacgtt tcccgttgaa tatggctcat 6421 aacacccctt gtattactgt ttatgtaagc agacagtttt attgttcatg atgatatatt 6481 tttatcttgt gcaatgtaac atcagagatt ttgagacaca acgtggcttt gttgaataaa 6541 tcgaactttt gctgagttga aggatcagct cgagttacca atgcttaatc agtgaggcac 6601 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga 6661 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc 6721 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca 6781 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta 6841 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg 6901 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc 6961 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg 7021 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt 7081 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt 7141 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata 7201 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc 7261 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac 7321 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa 7381 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct 7441 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat 7501 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc 7561 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca 7621 cgaggcagaa tttcagataa aaaaaatcct tagctttcgc taaggatgat ttctggaatt 7681 cgcggccgct tctagag //