BBa_J153001
1
BBa_J153001
Ptrc1O
2012-02-03T12:00:00Z
2015-08-31T04:08:32Z
Ptrc1O, or the trc promoter, was extracted from the pTrc99A plasmid using PCR (Huang etal 2010, Brosius etal 1985) (links).
Ptrc1O, or the trc promoter, is a hybrid of the Escherichia coli trp promoter and the lacUV5 promoter (Brosius 1985).
It contains the O1 lac operator (proximal to the core promoter) that allows for LacI repression and subsequent induction by IPTG.
Usage
It was characterized with a GFP reporter construct (link) carried on the broad-host-range shuttle vector pPMQAK1 (link) to be stronger than both ???Plac??? (link) (ca 35% stronger) and ???PtetR??? (link) (ca 320 % stronger) in E. coli DH5alpha. Characterization in the cyanobacterium Synechocystis sp. PCC 6803 revealed strong fluorescence from the Ptrc1O-driven construct but no detectable signal from the ???Plac??? construct and only a weak signal from the ???PtetR??? construct. Repression of Ptrc1O by LacI in Synechocystis was very modest (Huang etal 2010) (link).
The Ptrc2O promoter (link) is of similar strength as Ptrc1O but significantly more repressed by LacI in Synechocystis. However, there are problems inducing this promoter to its full strength, limiting its usability.
false
false
_643_
0
1292
265
It's complicated
false
None
false
Daniel Camsund / Hsinho Huang
annotation2170088
1
-10 box
range2170088
1
24
29
annotation2170089
1
lac O1
range2170089
1
36
56
annotation2170087
1
-35 box
range2170087
1
1
6
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation4184
1
stem_loop
range4184
1
12
55
annotation7018
1
BBa_B0010
range7018
1
1
80
BBa_I13504
1
BBa_I13504
Screening plasmid intermediate
2005-05-30T11:00:00Z
2015-08-31T04:07:34Z
Released HQ 2013
Built by Josh as an intermediate in screening plasmid construction.
false
true
_11_
0
253
6
In stock
false
true
jkm
component1505084
1
BBa_B0012
component1505067
1
BBa_B0034
component1505069
1
BBa_E0040
component1505074
1
BBa_B0010
annotation1505084
1
BBa_B0012
range1505084
1
835
875
annotation1505067
1
BBa_B0034
range1505067
1
1
12
annotation1505069
1
BBa_E0040
range1505069
1
19
738
annotation1505074
1
BBa_B0010
range1505074
1
747
826
BBa_E0040
1
GFP
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
2004-09-29T11:00:00Z
2016-01-26T02:09:38Z
Released HQ 2013
GFP (mut3b) [note that this part does not have a barcode]
false
true
_11_1_
4206
61
7
In stock
false
true
jcbraff
annotation1934520
1
GFP protein
range1934520
1
1
720
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation7020
1
BBa_B0012
range7020
1
1
41
BBa_J153003
1
BBa_J153003
Ptrc1O-driven GFP reporter
2012-02-03T12:00:00Z
2015-08-31T04:08:32Z
This Ptrc1O-driven GFP reporter was assembled from Ptrc1O (link) and an intermediate GFP construct (Part:BBa_I13504).
Uses the strong Ptrc1O promoter (link) to generate untagged GFPmut3b (link).
Usage
This strong GFP generator was used to test for the replication of the broad-host-range shuttle vector pPMQAK1 (link) in various cyanobacteria (Huang etal 2010) (link). This reporter carried on pPMQAK1 has also been used as a reference construct for free cytoplasmic GFP in cyanobacterial protein localization studies (Camsund etal 2011) (link).
false
false
_643_
0
1292
265
It's complicated
false
None
false
Daniel Camsund / Hsinho Huang
component2170122
1
BBa_I13504
component2170112
1
BBa_J153001
annotation2170112
1
BBa_J153001
range2170112
1
1
63
annotation2170122
1
BBa_I13504
range2170122
1
72
946
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_J153003_sequence
1
ttgacaattaatcatccggctcgtataatgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0034_sequence
1
aaagaggagaaa
BBa_J153001_sequence
1
ttgacaattaatcatccggctcgtataatgtgtggaattgtgagcggataacaatttcacaca
BBa_E0040_sequence
1
atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
BBa_I13504_sequence
1
aaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z