BBa_J15402 1 BBa_J15402 ars promoter + rbs + lacZ' 2007-07-12T11:00:00Z 2015-08-31T04:08:32Z The E. coli chromosomal ars promoter comes from E. coli K12 (gi: 510824), the lacZ' from E. coli BS (gi: 146575). This is the Escherichia coli chromosomal ars promoter attached to a lacZ' gene encoding the N-terminus of LacZ (beta galactosidase). Note that the promoter is not effectively regulated without a source of ArsR protein. false false _163_ 0 837 163 Not in stock false No issues. false Chris French component1938098 1 BBa_J15301 component1938105 1 BBa_J15102 component1938101 1 BBa_J15001 annotation1938105 1 BBa_J15102 range1938105 1 152 385 annotation1938098 1 BBa_J15301 range1938098 1 1 127 annotation1938101 1 BBa_J15001 range1938101 1 136 145 BBa_J15102 1 BBa_J15102 lacZ' coding sequence 2007-07-12T11:00:00Z 2015-08-31T04:08:32Z Escherichia coli BS. The sequence was obtained from gi:146575. Released HQ 2013 Escherichia coli lacZ' coding sequence encoding the first 76 amino acids of LacZ (beta-galactosidase). This is sufficient to complement the lacZ-delta-M15 mutation found in commonly used laboratory strains of E. coli, thus allowing blue-white selection. It also can be used to effect a pH drop in the presence of lactose, as in the arsenic biosensor entered by the University of Ediburgh team for iGEM2006. false false _163_ 0 837 163 In stock false Codon 77, TGC, was changed to TAA to truncate the coding sequence. false Chris French annotation1938052 1 start lacZ' range1938052 1 1 3 annotation1938053 1 end lacZ range1938053 1 229 231 annotation1938051 1 lacZ' range1938051 1 1 231 BBa_J15001 1 BBa_J15001 strong synthetic E. coli ribosome binding site with SacI site. 2007-07-12T11:00:00Z 2015-08-31T04:08:32Z Synthetic. This is a strong synthetic E. coli ribosome binding site. It is synthesised as two complementary oligonucleotides rather than being incorporated into a biobrick plasmid. It incorporates a SacI site overlapping the XbaI site, which is preserved when it is added to any other biobrick. This allows easy detection of the RBS after it has been added upstream of a biobrick coding sequence in a plasmid vector. false false _163_ 0 837 163 Not in stock false Note the presence of a SacI site overlapping the XbaI site, which is preserved when this biobrick is added to any other biobrick. At the time of writing, this biobrick is added as a short piece of DNA composed of two complementary oligonucleotides rather than being incorporated into a biobrick cloning vector by itself. It can be added upstream of a biobrick coding sequence, and its presence can easily be detected in miniprep DNA on a gel by using a SacI-SpeI or similar digest. false Chris French annotation1938045 1 SacI range1938045 1 1 3 annotation1938046 1 rbs range1938046 1 4 10 BBa_J15301 1 BBa_J15301 Pars promoter from Escherichia coli chromosomal ars operon. 2007-07-12T11:00:00Z 2015-08-31T04:08:32Z Escherichia coli K12. Sequence obtained from X80057 (gi:510824). Regulatory sequence annotation from Cai, J., and DuBow, M.S. 1996. Expression of the Escherichia coli chromosomal ars operon. Canadian Journal of Microbiology 42, 662-671; Diorio, C., Cai, J., Marmor, J., Shinder, R., and DuBow, M.S. 1995. An Escherichia coli chromosomal ars operon homolog is functional in arsenic detoxification and is conserved in Gram negative bacteria. Journal of Bacteriology 177, 2050-2056. This is the promoter region of the Escherichia coli chromosomal ars promoter. It is repressed by ArsR in the absence of arsenate or arsenite (Cai, J., and DuBow, M.S. 1996. Expression of the Escherichia coli chromosomal ars operon. Canadian Journal of Microbiology 42, 662-671; Diorio, C., Cai, J., Marmor, J., Shinder, R., and DuBow, M.S. 1995. An Escherichia coli chromosomal ars operon homolog is functional in arsenic detoxification and is conserved in Gram negative bacteria. Journal of Bacteriology 177, 2050-2056). false false _163_ 0 837 163 Not in stock false No modifications were made to the sequence. There appears to be an unexpected repeat of ACT immediately before the SpeI site. This does not affect the biobrick suffix but means that the suffix is 5 bases downstream of the annotated transcriptional start site rather than 2 as recommended. The reason for this is unclear. false Chris French annotation1938076 1 -35 range1938076 1 88 93 annotation1938075 1 ArsR binding range1938075 1 81 85 annotation1938078 1 +1 range1938078 1 123 123 annotation1938077 1 -10 range1938077 1 111 116 annotation1938074 1 ArsR binding range1938074 1 67 71 BBa_J15102_sequence 1 atgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtaataa BBa_J15001_sequence 1 ctcaaggagg BBa_J15402_sequence 1 tcctgattcagacctcctttcaaatgaatagccaactcaaaattcacacctattaccttcctctgcacttacacattcgttaagtcatatatgtttttgacttatccgcttcgaagagagacactactactagagctcaaggaggtactagatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtaataa BBa_J15301_sequence 1 tcctgattcagacctcctttcaaatgaatagccaactcaaaattcacacctattaccttcctctgcacttacacattcgttaagtcatatatgtttttgacttatccgcttcgaagagagacactac igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z