LOCUS       BBa_J15509              3434 bp    DNA     linear       29-Nov-2024
DEFINITION  Plac+RBS+cex+RBS+cenA
ACCESSION   BBa_J15509
VERSION     BBa_J15509.1  
FEATURES             Location/Qualifiers
     misc_feature    1..3
                     /label=SacI
     misc_feature    248..285
                     /label=CAP binding site
     misc_feature    332..352
                     /label=LacI binding site
     RBS             359..362
                     /label=rbs
     CDS             370..600
                     /label=lacZ'
     promoter        320..325
                     /label=-10
     promoter        297..302
                     /label=-35
     misc_feature    1..600
                     /label=BBa_J33207
     misc_feature    601..603
                     /label=SacI
     RBS             604..610
                     /label=rbs
     RBS             601..610
                     /label=BBa_J15001
     CDS             611..2068
                     /label=cex coding sequence
     misc_feature    1391..1396
                     /label=PstI site removed
     CDS             611..2071
                     /label=BBa_K118022
     misc_feature    601..2071
                     /label=BBa_J15507
     misc_feature    2072..2074
                     /label=SacI
     RBS             2075..2081
                     /label=rbs
     RBS             2072..2081
                     /label=BBa_J15001
     CDS             2082..3431
                     /label=cenA coding sequence
     CDS             2082..3434
                     /label=BBa_K118023
     misc_feature    2072..3434
                     /label=BBa_J15508
ORIGIN
        1 ctcatgttat atcccgccgt taaccaccat caaacaggat tttcgcctgc tggggcaaac
       61 cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt
      121 gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc
      181 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
      241 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
      301 actttatgct tccggctcgt atgttgtgtg aaattgtgag cggataacaa tttcacacag
      361 gaaacagcta tgaccatgat tacggattca ctggccgtcg ttttacaacg tcgtgactgg
      421 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
      481 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
      541 gaatggcgct ttgcctggtt tccggcacca gaagcggtgc cggaaagctg gctggagtga
      601 ctcaaggagg atgcctagga ccacgcccgc acccggccac ccggcccgcg gcgcccgcac
      661 cgctctgcgc acgacgctcg ccgccgcggc ggcgacgctc gtcgtcggcg ccacggtcgt
      721 gctgcccgcc caggccgcga ccacgctcaa ggaggccgcc gacggcgccg gccgggactt
      781 cggcttcgcg ctcgacccca accggctctc ggaggcgcag tacaaggcga tcgccgacag
      841 cgagttcaac ctcgtcgtcg ccgagaacgc gatgaagtgg gacgccaccg agccctcgca
      901 gaacagcttc tccttcggcg cgggcgaccg cgtcgcgagc tacgccgccg acaccggcaa
      961 ggagctgtac ggccacacgc tcgtatggca ctcgcagctg cccgactggg cgaagaacct
     1021 caacggctcc gcgttcgaga gcgcgatggt caaccacgtg acgaaggtcg ccgaccactt
     1081 cgagggcaag gtcgcgtcgt gggacgtcgt caacgaggcg ttcgccgacg gcggcggccg
     1141 ccggcaggac tcggcgttcc agcagaagct cggcaacggc tacatcgaga ccgcgttccg
     1201 ggcggcacgt gcggcggacc cgaccgccaa gctgtgcatc aacgactaca acgtcgaggg
     1261 catcaacgcg aagagcaact cgctctacga cctcgtcaag gacttcaagg cgcgcggcgt
     1321 cccgctcgac tgcgtcgggt tccagtcgca cctcatcgtc ggccaggtgc cgggcgactt
     1381 ccggcagaac ctgcaacggt tcgcggacct gggcgtggac gtgcgcatca ccgagctcga
     1441 catccgcatg cggacgccct ccgacgcgac caagctcgcg acccaggcgg ccgactacaa
     1501 gaaggtcgtg caggcctgca tgcaggtgac ccgctgccag ggcgtgaccg tctggggcat
     1561 caccgacaag tactcgtggg tgccggacgt cttcccgggc gagggggccg cgctggtgtg
     1621 ggacgcgagc tacgccaaga agccggccta cgccgccgtg atggaggcct tcggcgcgag
     1681 cccgacgccg acgcccacca cgccgacccc gacgcccacg acgccgacgc cgaccccgac
     1741 gtccggtccg gccgggtgcc aggtgctgtg gggcgtcaac cagtggaaca ccggcttcac
     1801 cgcgaacgtc accgtgaaga acacgtcctc cgctccggtc gacggctgga cgctcacgtt
     1861 cagcttcccg tccggccagc aggtcaccca ggcgtggagc tcgacggtca cgcagtccgg
     1921 ctcggccgtg acggtccgca acgccccgtg gaacggctcg atcccggcgg gcggcaccgc
     1981 gcagttcggc ttcaacggct cgcacacggg caccaacgcc gcgccgacgg cgttctcgct
     2041 caacggcacg ccctgcacgg tcggctaata actcaaggag gatgtccacc cgcagaaccg
     2101 ccgcagcgct gctggcggcc gcggccgtcg ccgtcggcgg tctgaccgcc ctcaccacca
     2161 ccgccgcgca ggcggctccc ggctgccgcg tcgactacgc cgtcaccaac cagtggcccg
     2221 gcggcttcgg cgccaacgtc acgatcacca acctcggcga ccccgtctcg tcgtggaagc
     2281 tcgactggac ctacaccgca ggccagcgga tccagcagct gtggaacggc accgcgtcga
     2341 ccaacggcgg ccaggtctcc gtcaccagcc tgccctggaa cggcagcatc ccgaccggcg
     2401 gcacggcgtc gttcgggttc aacggctcgt gggccgggtc caacccgacg ccggcgtcgt
     2461 tctcgctcaa cggcaccacc tgcacgggca ccgtgccgac gaccagcccc acgccgaccc
     2521 cgacgccgac gacccccacg ccgacgccga ccccgacccc cacccccacg ccgacggtca
     2581 cgccgcagcc gaccagcggc ttctacgtcg acccgacgac gcagggctac cgcgcgtggc
     2641 aggccgcgtc cggcacggac aaggcgctgc tcgagaagat cgcgctcacc ccgcaggcgt
     2701 actgggtcgg caactgggcc gacgcgtcgc acgcgcaggc cgaggtcgcc gactacaccg
     2761 gccgcgccgt cgcggccggg aagacgccga tgctcgtcgt ctacgcgatc ccgggccgcg
     2821 actgcggctc gcactccggc ggtggtgtgt ccgagtccga gtacgcgcgc tgggtcgaca
     2881 ccgtcgcgca gggcatcaag ggcaacccga tcgtgatcct cgagcccgac gcgctcgcgc
     2941 agctcggcga ctgctccggc cagggtgacc gcgtcggctt cctcaagtac gccgccaagt
     3001 cgctcaccct caagggcgcg cgcgtctaca tcgacgcggg ccacgcgaag tggctgtcgg
     3061 tcgacacgcc ggtgaaccgc ctcaaccagg tcggcttcga gtacgcggtg ggcttcgcgc
     3121 tcaacacgtc gaactaccag acgacggcgg acagcaaggc gtacggccag cagatctcgc
     3181 agcggctggg cggcaagaag ttcgtcatcg acacctcgcg caacggcaac ggctcgaacg
     3241 gcgagtggtg caacccgcgc ggccgcgcgc tcggcgaacg cccggtcgcg gtgaacgacg
     3301 gctccggcct ggacgcgctc ctgtgggtca agctgcccgg cgagtccgac ggcgcgtgca
     3361 acggcggccc ggccgccggc cagtggtggc aggagatcgc cctggagatg gcgcgcaacg
     3421 ccaggtggta ataa
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