##gff-version 3
##sequence-region BBa_J15509 1 3434
BBa_J15509	.	engineered_region	1	600	.	+	0	ID=BBa_J33207;Name=BBa_J33207
BBa_J15509	.	ribosome_entry_site	359	362	.	+	0	ID=annotation1907857;Name=rbs;Parent=BBa_J33207
BBa_J15509	.	CDS	370	600	.	+	0	ID=annotation1907858;Name=lacZ';Parent=BBa_J33207
BBa_J15509	.	sequence_feature	1	3	.	+	0	ID=annotation1907854;Name=SacI;Parent=BBa_J33207
BBa_J15509	.	operator	248	285	.	+	0	ID=annotation1907855;Name=CAP binding site;Parent=BBa_J33207
BBa_J15509	.	operator	332	352	.	+	0	ID=annotation1907856;Name=LacI binding site;Parent=BBa_J33207
BBa_J15509	.	promoter	320	325	.	+	0	ID=annotation1907859;Name=-10;Parent=BBa_J33207
BBa_J15509	.	promoter	297	302	.	+	0	ID=annotation1907860;Name=-35;Parent=BBa_J33207
BBa_J15509	.	engineered_region	601	2071	.	+	0	ID=BBa_J15507;Name=BBa_J15507
BBa_J15509	.	ribosome_entry_site	601	610	.	+	0	ID=BBa_J15001;Name=BBa_J15001;Parent=BBa_J15507
BBa_J15509	.	ribosome_entry_site	604	610	.	+	0	ID=annotation1938046;Name=rbs;Parent=BBa_J15001
BBa_J15509	.	sequence_feature	601	603	.	+	0	ID=annotation1938045;Name=SacI;Parent=BBa_J15001
BBa_J15509	.	CDS	611	2071	.	+	0	ID=BBa_K118022;Name=BBa_K118022;Parent=BBa_J15507
BBa_J15509	.	CDS	611	2068	.	+	0	ID=annotation1996993;Name=cex coding sequence;Parent=BBa_K118022
BBa_J15509	.	point_mutation	1391	1396	.	+	0	ID=annotation1996994;Name=PstI site removed;Parent=BBa_K118022
BBa_J15509	.	engineered_region	2072	3434	.	+	0	ID=BBa_J15508;Name=BBa_J15508
BBa_J15509	.	ribosome_entry_site	2072	2081	.	+	0	ID=BBa_J15001;Name=BBa_J15001;Parent=BBa_J15508
BBa_J15509	.	ribosome_entry_site	2075	2081	.	+	0	ID=annotation1938046;Name=rbs;Parent=BBa_J15001
BBa_J15509	.	sequence_feature	2072	2074	.	+	0	ID=annotation1938045;Name=SacI;Parent=BBa_J15001
BBa_J15509	.	CDS	2082	3434	.	+	0	ID=BBa_K118023;Name=BBa_K118023;Parent=BBa_J15508
BBa_J15509	.	CDS	2082	3431	.	+	0	ID=annotation1996991;Name=cenA coding sequence;Parent=BBa_K118023
>BBa_J15509
ctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctg
caactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgc
ccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaag
cgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggc
tcgtatgttgtgtgaaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacggattcactggc
cgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgcc
agctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttg
cctggtttccggcaccagaagcggtgccggaaagctggctggagtgactcaaggaggatgcctaggaccacgcccgcac
ccggccacccggcccgcggcgcccgcaccgctctgcgcacgacgctcgccgccgcggcggcgacgctcgtcgtcggcgc
cacggtcgtgctgcccgcccaggccgcgaccacgctcaaggaggccgccgacggcgccggccgggacttcggcttcgcg
ctcgaccccaaccggctctcggaggcgcagtacaaggcgatcgccgacagcgagttcaacctcgtcgtcgccgagaacg
cgatgaagtgggacgccaccgagccctcgcagaacagcttctccttcggcgcgggcgaccgcgtcgcgagctacgccgc
cgacaccggcaaggagctgtacggccacacgctcgtatggcactcgcagctgcccgactgggcgaagaacctcaacggc
tccgcgttcgagagcgcgatggtcaaccacgtgacgaaggtcgccgaccacttcgagggcaaggtcgcgtcgtgggacg
tcgtcaacgaggcgttcgccgacggcggcggccgccggcaggactcggcgttccagcagaagctcggcaacggctacat
cgagaccgcgttccgggcggcacgtgcggcggacccgaccgccaagctgtgcatcaacgactacaacgtcgagggcatc
aacgcgaagagcaactcgctctacgacctcgtcaaggacttcaaggcgcgcggcgtcccgctcgactgcgtcgggttcc
agtcgcacctcatcgtcggccaggtgccgggcgacttccggcagaacctgcaacggttcgcggacctgggcgtggacgt
gcgcatcaccgagctcgacatccgcatgcggacgccctccgacgcgaccaagctcgcgacccaggcggccgactacaag
aaggtcgtgcaggcctgcatgcaggtgacccgctgccagggcgtgaccgtctggggcatcaccgacaagtactcgtggg
tgccggacgtcttcccgggcgagggggccgcgctggtgtgggacgcgagctacgccaagaagccggcctacgccgccgt
gatggaggccttcggcgcgagcccgacgccgacgcccaccacgccgaccccgacgcccacgacgccgacgccgaccccg
acgtccggtccggccgggtgccaggtgctgtggggcgtcaaccagtggaacaccggcttcaccgcgaacgtcaccgtga
agaacacgtcctccgctccggtcgacggctggacgctcacgttcagcttcccgtccggccagcaggtcacccaggcgtg
gagctcgacggtcacgcagtccggctcggccgtgacggtccgcaacgccccgtggaacggctcgatcccggcgggcggc
accgcgcagttcggcttcaacggctcgcacacgggcaccaacgccgcgccgacggcgttctcgctcaacggcacgccct
gcacggtcggctaataactcaaggaggatgtccacccgcagaaccgccgcagcgctgctggcggccgcggccgtcgccg
tcggcggtctgaccgccctcaccaccaccgccgcgcaggcggctcccggctgccgcgtcgactacgccgtcaccaacca
gtggcccggcggcttcggcgccaacgtcacgatcaccaacctcggcgaccccgtctcgtcgtggaagctcgactggacc
tacaccgcaggccagcggatccagcagctgtggaacggcaccgcgtcgaccaacggcggccaggtctccgtcaccagcc
tgccctggaacggcagcatcccgaccggcggcacggcgtcgttcgggttcaacggctcgtgggccgggtccaacccgac
gccggcgtcgttctcgctcaacggcaccacctgcacgggcaccgtgccgacgaccagccccacgccgaccccgacgccg
acgacccccacgccgacgccgaccccgacccccacccccacgccgacggtcacgccgcagccgaccagcggcttctacg
tcgacccgacgacgcagggctaccgcgcgtggcaggccgcgtccggcacggacaaggcgctgctcgagaagatcgcgct
caccccgcaggcgtactgggtcggcaactgggccgacgcgtcgcacgcgcaggccgaggtcgccgactacaccggccgc
gccgtcgcggccgggaagacgccgatgctcgtcgtctacgcgatcccgggccgcgactgcggctcgcactccggcggtg
gtgtgtccgagtccgagtacgcgcgctgggtcgacaccgtcgcgcagggcatcaagggcaacccgatcgtgatcctcga
gcccgacgcgctcgcgcagctcggcgactgctccggccagggtgaccgcgtcggcttcctcaagtacgccgccaagtcg
ctcaccctcaagggcgcgcgcgtctacatcgacgcgggccacgcgaagtggctgtcggtcgacacgccggtgaaccgcc
tcaaccaggtcggcttcgagtacgcggtgggcttcgcgctcaacacgtcgaactaccagacgacggcggacagcaaggc
gtacggccagcagatctcgcagcggctgggcggcaagaagttcgtcatcgacacctcgcgcaacggcaacggctcgaac
ggcgagtggtgcaacccgcgcggccgcgcgctcggcgaacgcccggtcgcggtgaacgacggctccggcctggacgcgc
tcctgtgggtcaagctgcccggcgagtccgacggcgcgtgcaacggcggcccggccgccggccagtggtggcaggagat
cgccctggagatggcgcgcaacgccaggtggtaataa