BBa_J15509
type: ComponentDefinition
persistent identity: BBa J15509
display id: BBa_J15509
version: 1
was generated by: iGEM to SBOL conversion
description: Plac+RBS+cex+RBS+cenA
created: 2013-11-25T12:00:00Z
modified: 2015-08-31T04:08:33Z
mutable provenance: Cellulomonas fimi
owned by: james
owned by: myers
mutable description: Exo- and endo- glucanases from the cellulolytic bacterium Cellulomonas fimi under the E. coli lac promoter. Together with betaglucosidases, the bacterium degrades cellulose into D-glucose.
discontinued: false
dominant: false
experience: experience/None
group u list: _163_
m user id: 0
owner id: 14847
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Cex is a GC rich part and may require extended denaturation in amplifying the gene.
star: false
creator: Kwabena O. Duedu, Chris French, Sahreena Lakhundi
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J15508
component: BBa_J15507
component: BBa_J33207
sequence annotation: BBa_J33207
sequence annotation: BBa_J15507
sequence annotation: BBa_J15508
sequence: https://synbiohub.org/public/igem/BBa_J15509_sequence/1

BBa_J15508
type: Component
persistent identity: component2371462
display id: component2371462
version: 1
top level: BBa_J15509
definition: BBa_J15508
access: public

BBa_J15507
type: Component
persistent identity: component2371456
display id: component2371456
version: 1
top level: BBa_J15509
definition: BBa_J15507
access: public

BBa_J33207
type: Component
persistent identity: component2371449
display id: component2371449
version: 1
top level: BBa_J15509
definition: BBa_J33207
access: public

BBa_J33207
type: SequenceAnnotation
persistent identity: annotation2371449
display id: annotation2371449
version: 1
top level: BBa_J15509
location: https://synbiohub.org/public/igem/BBa_J15509/annotation2371449/range2371449/1
component: BBa_J33207

https://synbiohub.org/public/igem/BBa_J15509/annotation2371449/range2371449/1
type: Range
persistent identity: range2371449
display id: range2371449
version: 1
top level: BBa_J15509
direction: direction/reverse
start: 1
end: 600
orientation: inline

BBa_J15507
type: SequenceAnnotation
persistent identity: annotation2371456
display id: annotation2371456
version: 1
top level: BBa_J15509
location: https://synbiohub.org/public/igem/BBa_J15509/annotation2371456/range2371456/1
component: BBa_J15507

https://synbiohub.org/public/igem/BBa_J15509/annotation2371456/range2371456/1
type: Range
persistent identity: range2371456
display id: range2371456
version: 1
top level: BBa_J15509
direction: direction/reverse
start: 601
end: 2071
orientation: inline

BBa_J15508
type: SequenceAnnotation
persistent identity: annotation2371462
display id: annotation2371462
version: 1
top level: BBa_J15509
location: https://synbiohub.org/public/igem/BBa_J15509/annotation2371462/range2371462/1
component: BBa_J15508

https://synbiohub.org/public/igem/BBa_J15509/annotation2371462/range2371462/1
type: Range
persistent identity: range2371462
display id: range2371462
version: 1
top level: BBa_J15509
direction: direction/reverse
start: 2072
end: 3434
orientation: inline

BBa_J33207
type: ComponentDefinition
persistent identity: BBa J33207
display id: BBa_J33207
version: 1
was generated by: iGEM to SBOL conversion
description: lac promoter and lacZ
created: 2006-10-26T11:00:00Z
modified: 2015-08-31T04:08:46Z
mutable provenance: The DNA was amplified from E. coli BL21 genomic DNA using primers based on published sequence (Genbank accession J01636, gi:146575). The annotation shown here is based on that associated with this Genbank entry. The sequence shown here is derived by sequencing the construct.
owned by: james
owned by: myers
mutable description: This part (submitted in pSB1A2) consists of the lac promoter and lacZ' gene, encoding the N-terminal 76 amino acid residues of LacZ, sufficient to complement the lacZ-delta-M15 mutation for blue-white selection on Xgal plates. A SacI site has been introduced at the 3' end, overlapping the XbaI site of the Biobrick prefix. This is designed to be used as a cloning vector for making new biobricks. PCR primers can be designed with a SacI site in one primer and an SpeI site in the other. This removes the necessity for an excessively long non-complementary tail on one primer bearing either the full biobrick prefix or suffix. The PCR product can then be digested with SacI and SpeI for insertion into this plasmid, replacing the Plac-lacZ' cassette. Recombinant plasmids will then be white on IPTG/Xgal plates, whereas any that still contain the original insert will be blue. We have used this strategy to prepare several biobricks, including BBa_J33204, which contains the xylE gene encoding catechol-2,3-dioxygenase. (For making biobricks that contain lacZ', BBa_J33204 can be used in the same way; in this case, clones with plasmids that still contain xylE will turn yellow on addition of a drop of 10 mM catechol.)
discontinued: false
dominant: false
experience: experience/Works
group u list: _63_
m user id: 0
owner id: 837
owning group id: 63
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: Note that the SacI site overlaps the SpeI site. The Biobrick prefix ends ...TCTAGAG. When this is added to the CTC at the start of the sequence shown here, the SacI site, GAGCTC, is generated.
star: false
creator: Chris French
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: SacI
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: rbs
sequence annotation: lacZ'
sequence: https://synbiohub.org/public/igem/BBa_J33207_sequence/1

SacI
type: SequenceAnnotation
persistent identity: annotation1907854
display id: annotation1907854
version: 1
top level: BBa_J33207
location: https://synbiohub.org/public/igem/BBa_J33207/annotation1907854/range1907854/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_J33207/annotation1907854/range1907854/1
type: Range
persistent identity: range1907854
display id: range1907854
version: 1
top level: BBa_J33207
direction: direction/forward
start: 1
end: 3
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1907855
display id: annotation1907855
version: 1
top level: BBa_J33207
location: https://synbiohub.org/public/igem/BBa_J33207/annotation1907855/range1907855/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_J33207/annotation1907855/range1907855/1
type: Range
persistent identity: range1907855
display id: range1907855
version: 1
top level: BBa_J33207
direction: direction/forward
start: 248
end: 285
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1907860
display id: annotation1907860
version: 1
top level: BBa_J33207
location: https://synbiohub.org/public/igem/BBa_J33207/annotation1907860/range1907860/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_J33207/annotation1907860/range1907860/1
type: Range
persistent identity: range1907860
display id: range1907860
version: 1
top level: BBa_J33207
direction: direction/forward
start: 297
end: 302
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1907859
display id: annotation1907859
version: 1
top level: BBa_J33207
location: https://synbiohub.org/public/igem/BBa_J33207/annotation1907859/range1907859/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_J33207/annotation1907859/range1907859/1
type: Range
persistent identity: range1907859
display id: range1907859
version: 1
top level: BBa_J33207
direction: direction/forward
start: 320
end: 325
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1907856
display id: annotation1907856
version: 1
top level: BBa_J33207
location: https://synbiohub.org/public/igem/BBa_J33207/annotation1907856/range1907856/1
role: feature/operator
role: SO:0000057

https://synbiohub.org/public/igem/BBa_J33207/annotation1907856/range1907856/1
type: Range
persistent identity: range1907856
display id: range1907856
version: 1
top level: BBa_J33207
direction: direction/forward
start: 332
end: 352
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation1907857
display id: annotation1907857
version: 1
top level: BBa_J33207
location: https://synbiohub.org/public/igem/BBa_J33207/annotation1907857/range1907857/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_J33207/annotation1907857/range1907857/1
type: Range
persistent identity: range1907857
display id: range1907857
version: 1
top level: BBa_J33207
direction: direction/forward
start: 359
end: 362
orientation: inline

lacZ'
type: SequenceAnnotation
persistent identity: annotation1907858
display id: annotation1907858
version: 1
top level: BBa_J33207
location: https://synbiohub.org/public/igem/BBa_J33207/annotation1907858/range1907858/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_J33207/annotation1907858/range1907858/1
type: Range
persistent identity: range1907858
display id: range1907858
version: 1
top level: BBa_J33207
direction: direction/forward
start: 370
end: 600
orientation: inline

BBa_J15507
type: ComponentDefinition
persistent identity: BBa J15507
display id: BBa_J15507
version: 1
was generated by: iGEM to SBOL conversion
description: RBS+Cex (Exoglucanase)
created: 2013-11-25T12:00:00Z
modified: 2015-08-31T04:08:33Z
mutable provenance: Cellulomonas fimi exoglucanase cex (accession M15824) with RBS
owned by: james
owned by: myers
mutable description: RBS + Exoglucanase (cex, BBa_K118022) from the cellulolytic bacterium Cellulomonas fimi (accession M15824).
discontinued: false
dominant: false
group u list: _163_
m user id: 0
owner id: 14847
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: GC rich part. Extended denaturation stage in PCR with glycerol or commercial GC rich enhancers may be required
star: false
creator: Kwabena O. Duedu
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K118022
component: BBa_J15001
sequence annotation: BBa_J15001
sequence annotation: BBa_K118022
sequence: https://synbiohub.org/public/igem/BBa_J15507_sequence/1

BBa_K118022
type: Component
persistent identity: component2371436
display id: component2371436
version: 1
top level: BBa_J15507
definition: BBa_K118022
access: public

BBa_J15001
type: Component
persistent identity: component2371433
display id: component2371433
version: 1
top level: BBa_J15507
definition: BBa_J15001
access: public

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation2371433
display id: annotation2371433
version: 1
top level: BBa_J15507
location: https://synbiohub.org/public/igem/BBa_J15507/annotation2371433/range2371433/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_J15507/annotation2371433/range2371433/1
type: Range
persistent identity: range2371433
display id: range2371433
version: 1
top level: BBa_J15507
direction: direction/reverse
start: 1
end: 10
orientation: inline

BBa_K118022
type: SequenceAnnotation
persistent identity: annotation2371436
display id: annotation2371436
version: 1
top level: BBa_J15507
location: https://synbiohub.org/public/igem/BBa_J15507/annotation2371436/range2371436/1
component: BBa_K118022

https://synbiohub.org/public/igem/BBa_J15507/annotation2371436/range2371436/1
type: Range
persistent identity: range2371436
display id: range2371436
version: 1
top level: BBa_J15507
direction: direction/reverse
start: 11
end: 1471
orientation: inline

BBa_J15508
type: ComponentDefinition
persistent identity: BBa J15508
display id: BBa_J15508
version: 1
was generated by: iGEM to SBOL conversion
description: RBS+CenA (Endoglucanase)
created: 2013-11-25T12:00:00Z
modified: 2015-08-31T04:08:33Z
mutable provenance: Cellulomonas fimi (accession M15823)
owned by: james
owned by: myers
mutable description: Endoglucanase A (CenA) from the cellulolytic bacterium Cellulomonas fimi (accession M15823) used in the degradation of cellulose into cellobiose, before using beta-glucosidase to catalyse the conversion of cellobiose to D-glucose.
discontinued: false
dominant: false
group u list: _163_
m user id: 0
owner id: 14847
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: No special conditions required
star: false
creator: Kwabena O. Duedu
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K118023
component: BBa_J15001
sequence annotation: BBa_J15001
sequence annotation: BBa_K118023
sequence: https://synbiohub.org/public/igem/BBa_J15508_sequence/1

BBa_K118023
type: Component
persistent identity: component2371441
display id: component2371441
version: 1
top level: BBa_J15508
definition: BBa_K118023
access: public

BBa_J15001
type: Component
persistent identity: component2371439
display id: component2371439
version: 1
top level: BBa_J15508
definition: BBa_J15001
access: public

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation2371439
display id: annotation2371439
version: 1
top level: BBa_J15508
location: https://synbiohub.org/public/igem/BBa_J15508/annotation2371439/range2371439/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_J15508/annotation2371439/range2371439/1
type: Range
persistent identity: range2371439
display id: range2371439
version: 1
top level: BBa_J15508
direction: direction/reverse
start: 1
end: 10
orientation: inline

BBa_K118023
type: SequenceAnnotation
persistent identity: annotation2371441
display id: annotation2371441
version: 1
top level: BBa_J15508
location: https://synbiohub.org/public/igem/BBa_J15508/annotation2371441/range2371441/1
component: BBa_K118023

https://synbiohub.org/public/igem/BBa_J15508/annotation2371441/range2371441/1
type: Range
persistent identity: range2371441
display id: range2371441
version: 1
top level: BBa_J15508
direction: direction/reverse
start: 11
end: 1363
orientation: inline

BBa_J15001
type: ComponentDefinition
persistent identity: BBa J15001
display id: BBa_J15001
version: 1
was generated by: iGEM to SBOL conversion
description: strong synthetic E. coli ribosome binding site with SacI site.
created: 2007-07-12T11:00:00Z
modified: 2015-08-31T04:08:32Z
mutable provenance: Synthetic.
owned by: james
owned by: myers
mutable description: This is a strong synthetic E. coli ribosome binding site. It is synthesised as two complementary oligonucleotides rather than being incorporated into a biobrick plasmid. It incorporates a SacI site overlapping the XbaI site, which is preserved when it is added to any other biobrick. This allows easy detection of the RBS after it has been added upstream of a biobrick coding sequence in a plasmid vector.
discontinued: false
dominant: false
experience: experience/None
group u list: _163_
m user id: 0
owner id: 837
owning group id: 163
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Note the presence of a SacI site overlapping the XbaI site, which is preserved when this biobrick is added to any other biobrick. At the time of writing, this biobrick is added as a short piece of DNA composed of two complementary oligonucleotides rather than being incorporated into a biobrick cloning vector by itself. It can be added upstream of a biobrick coding sequence, and its presence can easily be detected in miniprep DNA on a gel by using a SacI-SpeI or similar digest.
star: false
creator: Chris French
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: SacI
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_J15001_sequence/1

SacI
type: SequenceAnnotation
persistent identity: annotation1938045
display id: annotation1938045
version: 1
top level: BBa_J15001
location: https://synbiohub.org/public/igem/BBa_J15001/annotation1938045/range1938045/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_J15001/annotation1938045/range1938045/1
type: Range
persistent identity: range1938045
display id: range1938045
version: 1
top level: BBa_J15001
direction: direction/forward
start: 1
end: 3
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation1938046
display id: annotation1938046
version: 1
top level: BBa_J15001
location: https://synbiohub.org/public/igem/BBa_J15001/annotation1938046/range1938046/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_J15001/annotation1938046/range1938046/1
type: Range
persistent identity: range1938046
display id: range1938046
version: 1
top level: BBa_J15001
direction: direction/forward
start: 4
end: 10
orientation: inline

BBa_K118022
type: ComponentDefinition
persistent identity: BBa K118022
display id: BBa_K118022
version: 1
was generated by: iGEM to SBOL conversion
description: cex coding sequence encoding Cellulomonas fimi exoglucanase
created: 2008-10-06T11:00:00Z
modified: 2015-05-08T01:09:37Z
mutable provenance: Purified ''Cellulomonas fimi'' genomic DNA.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The fungus ''Cellulomonas fimi'' uses an exoglucanase (from ''cex'') along with 3 endoglucanases in the degradation of cellulose into cellobiose, before use B-glucosidase to catalyse the conversion of cellobiose to D-glucose.
discontinued: false
dominant: false
experience: experience/None
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: The original sequence had a PstI site for 781-786. We added the silent mutation CAG->CAA (Gln) by MABEL to remove this site.
star: true
creator: Andrew Hall
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: cex coding sequence
sequence annotation: PstI site removed
sequence: https://synbiohub.org/public/igem/BBa_K118022_sequence/1

cex coding sequence
type: SequenceAnnotation
persistent identity: annotation1996993
display id: annotation1996993
version: 1
top level: BBa_K118022
location: https://synbiohub.org/public/igem/BBa_K118022/annotation1996993/range1996993/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K118022/annotation1996993/range1996993/1
type: Range
persistent identity: range1996993
display id: range1996993
version: 1
top level: BBa_K118022
direction: direction/forward
start: 1
end: 1458
orientation: inline

PstI site removed
type: SequenceAnnotation
persistent identity: annotation1996994
display id: annotation1996994
version: 1
top level: BBa_K118022
location: https://synbiohub.org/public/igem/BBa_K118022/annotation1996994/range1996994/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_K118022/annotation1996994/range1996994/1
type: Range
persistent identity: range1996994
display id: range1996994
version: 1
top level: BBa_K118022
direction: direction/forward
start: 781
end: 786
orientation: inline

BBa_K118023
type: ComponentDefinition
persistent identity: BBa K118023
display id: BBa_K118023
version: 1
was generated by: iGEM to SBOL conversion
description: cenA coding sequence encoding Cellulomonas fimi endoglucanase A
created: 2008-10-06T11:00:00Z
modified: 2015-05-08T01:09:37Z
mutable provenance: Purified ''Cellulomonas fimi'' genomic DNA.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The cellulolytic bacterium ''Cellulomonas fimi'' uses 3 endoglucanase (including CenA) and an exoglucanase in the degradation of cellulose into cellobiose, before use B-glucosidase to catalyse the conversion of cellobiose to D-glucose.
discontinued: false
dominant: false
experience: experience/None
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: No special considerations.
star: true
creator: Andrew Hall
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: cenA coding sequence
sequence: https://synbiohub.org/public/igem/BBa_K118023_sequence/1

cenA coding sequence
type: SequenceAnnotation
persistent identity: annotation1996991
display id: annotation1996991
version: 1
top level: BBa_K118023
location: https://synbiohub.org/public/igem/BBa_K118023/annotation1996991/range1996991/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K118023/annotation1996991/range1996991/1
type: Range
persistent identity: range1996991
display id: range1996991
version: 1
top level: BBa_K118023
direction: direction/forward
start: 1
end: 1350
orientation: inline

https://synbiohub.org/public/igem/BBa_J15509_sequence/1
type: Sequence
persistent identity: BBa J15509 sequence
display id: BBa_J15509_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtgaaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtgactcaaggaggatgcctaggaccacgcccgcacccggccacccggcccgcggcgcccgcaccgctctgcgcacgacgctcgccgccgcggcggcgacgctcgtcgtcggcgccacggtcgtgctgcccgcccaggccgcgaccacgctcaaggaggccgccgacggcgccggccgggacttcggcttcgcgctcgaccccaaccggctctcggaggcgcagtacaaggcgatcgccgacagcgagttcaacctcgtcgtcgccgagaacgcgatgaagtgggacgccaccgagccctcgcagaacagcttctccttcggcgcgggcgaccgcgtcgcgagctacgccgccgacaccggcaaggagctgtacggccacacgctcgtatggcactcgcagctgcccgactgggcgaagaacctcaacggctccgcgttcgagagcgcgatggtcaaccacgtgacgaaggtcgccgaccacttcgagggcaaggtcgcgtcgtgggacgtcgtcaacgaggcgttcgccgacggcggcggccgccggcaggactcggcgttccagcagaagctcggcaacggctacatcgagaccgcgttccgggcggcacgtgcggcggacccgaccgccaagctgtgcatcaacgactacaacgtcgagggcatcaacgcgaagagcaactcgctctacgacctcgtcaaggacttcaaggcgcgcggcgtcccgctcgactgcgtcgggttccagtcgcacctcatcgtcggccaggtgccgggcgacttccggcagaacctgcaacggttcgcggacctgggcgtggacgtgcgcatcaccgagctcgacatccgcatgcggacgccctccgacgcgaccaagctcgcgacccaggcggccgactacaagaaggtcgtgcaggcctgcatgcaggtgacccgctgccagggcgtgaccgtctggggcatcaccgacaagtactcgtgggtgccggacgtcttcccgggcgagggggccgcgctggtgtgggacgcgagctacgccaagaagccggcctacgccgccgtgatggaggccttcggcgcgagcccgacgccgacgcccaccacgccgaccccgacgcccacgacgccgacgccgaccccgacgtccggtccggccgggtgccaggtgctgtggggcgtcaaccagtggaacaccggcttcaccgcgaacgtcaccgtgaagaacacgtcctccgctccggtcgacggctggacgctcacgttcagcttcccgtccggccagcaggtcacccaggcgtggagctcgacggtcacgcagtccggctcggccgtgacggtccgcaacgccccgtggaacggctcgatcccggcgggcggcaccgcgcagttcggcttcaacggct…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J33207_sequence/1
type: Sequence
persistent identity: BBa J33207 sequence
display id: BBa_J33207_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtgaaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J15507_sequence/1
type: Sequence
persistent identity: BBa J15507 sequence
display id: BBa_J15507_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctcaaggaggatgcctaggaccacgcccgcacccggccacccggcccgcggcgcccgcaccgctctgcgcacgacgctcgccgccgcggcggcgacgctcgtcgtcggcgccacggtcgtgctgcccgcccaggccgcgaccacgctcaaggaggccgccgacggcgccggccgggacttcggcttcgcgctcgaccccaaccggctctcggaggcgcagtacaaggcgatcgccgacagcgagttcaacctcgtcgtcgccgagaacgcgatgaagtgggacgccaccgagccctcgcagaacagcttctccttcggcgcgggcgaccgcgtcgcgagctacgccgccgacaccggcaaggagctgtacggccacacgctcgtatggcactcgcagctgcccgactgggcgaagaacctcaacggctccgcgttcgagagcgcgatggtcaaccacgtgacgaaggtcgccgaccacttcgagggcaaggtcgcgtcgtgggacgtcgtcaacgaggcgttcgccgacggcggcggccgccggcaggactcggcgttccagcagaagctcggcaacggctacatcgagaccgcgttccgggcggcacgtgcggcggacccgaccgccaagctgtgcatcaacgactacaacgtcgagggcatcaacgcgaagagcaactcgctctacgacctcgtcaaggacttcaaggcgcgcggcgtcccgctcgactgcgtcgggttccagtcgcacctcatcgtcggccaggtgccgggcgacttccggcagaacctgcaacggttcgcggacctgggcgtggacgtgcgcatcaccgagctcgacatccgcatgcggacgccctccgacgcgaccaagctcgcgacccaggcggccgactacaagaaggtcgtgcaggcctgcatgcaggtgacccgctgccagggcgtgaccgtctggggcatcaccgacaagtactcgtgggtgccggacgtcttcccgggcgagggggccgcgctggtgtgggacgcgagctacgccaagaagccggcctacgccgccgtgatggaggccttcggcgcgagcccgacgccgacgcccaccacgccgaccccgacgcccacgacgccgacgccgaccccgacgtccggtccggccgggtgccaggtgctgtggggcgtcaaccagtggaacaccggcttcaccgcgaacgtcaccgtgaagaacacgtcctccgctccggtcgacggctggacgctcacgttcagcttcccgtccggccagcaggtcacccaggcgtggagctcgacggtcacgcagtccggctcggccgtgacggtccgcaacgccccgtggaacggctcgatcccggcgggcggcaccgcgcagttcggcttcaacggctcgcacacgggcaccaacgccgcgccgacggcgttctcgctcaacggcacgccctgcacggtcggctaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J15508_sequence/1
type: Sequence
persistent identity: BBa J15508 sequence
display id: BBa_J15508_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctcaaggaggatgtccacccgcagaaccgccgcagcgctgctggcggccgcggccgtcgccgtcggcggtctgaccgccctcaccaccaccgccgcgcaggcggctcccggctgccgcgtcgactacgccgtcaccaaccagtggcccggcggcttcggcgccaacgtcacgatcaccaacctcggcgaccccgtctcgtcgtggaagctcgactggacctacaccgcaggccagcggatccagcagctgtggaacggcaccgcgtcgaccaacggcggccaggtctccgtcaccagcctgccctggaacggcagcatcccgaccggcggcacggcgtcgttcgggttcaacggctcgtgggccgggtccaacccgacgccggcgtcgttctcgctcaacggcaccacctgcacgggcaccgtgccgacgaccagccccacgccgaccccgacgccgacgacccccacgccgacgccgaccccgacccccacccccacgccgacggtcacgccgcagccgaccagcggcttctacgtcgacccgacgacgcagggctaccgcgcgtggcaggccgcgtccggcacggacaaggcgctgctcgagaagatcgcgctcaccccgcaggcgtactgggtcggcaactgggccgacgcgtcgcacgcgcaggccgaggtcgccgactacaccggccgcgccgtcgcggccgggaagacgccgatgctcgtcgtctacgcgatcccgggccgcgactgcggctcgcactccggcggtggtgtgtccgagtccgagtacgcgcgctgggtcgacaccgtcgcgcagggcatcaagggcaacccgatcgtgatcctcgagcccgacgcgctcgcgcagctcggcgactgctccggccagggtgaccgcgtcggcttcctcaagtacgccgccaagtcgctcaccctcaagggcgcgcgcgtctacatcgacgcgggccacgcgaagtggctgtcggtcgacacgccggtgaaccgcctcaaccaggtcggcttcgagtacgcggtgggcttcgcgctcaacacgtcgaactaccagacgacggcggacagcaaggcgtacggccagcagatctcgcagcggctgggcggcaagaagttcgtcatcgacacctcgcgcaacggcaacggctcgaacggcgagtggtgcaacccgcgcggccgcgcgctcggcgaacgcccggtcgcggtgaacgacggctccggcctggacgcgctcctgtgggtcaagctgcccggcgagtccgacggcgcgtgcaacggcggcccggccgccggccagtggtggcaggagatcgccctggagatggcgcgcaacgccaggtggtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J15001_sequence/1
type: Sequence
persistent identity: BBa J15001 sequence
display id: BBa_J15001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctcaaggagg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K118022_sequence/1
type: Sequence
persistent identity: BBa K118022 sequence
display id: BBa_K118022_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcctaggaccacgcccgcacccggccacccggcccgcggcgcccgcaccgctctgcgcacgacgctcgccgccgcggcggcgacgctcgtcgtcggcgccacggtcgtgctgcccgcccaggccgcgaccacgctcaaggaggccgccgacggcgccggccgggacttcggcttcgcgctcgaccccaaccggctctcggaggcgcagtacaaggcgatcgccgacagcgagttcaacctcgtcgtcgccgagaacgcgatgaagtgggacgccaccgagccctcgcagaacagcttctccttcggcgcgggcgaccgcgtcgcgagctacgccgccgacaccggcaaggagctgtacggccacacgctcgtatggcactcgcagctgcccgactgggcgaagaacctcaacggctccgcgttcgagagcgcgatggtcaaccacgtgacgaaggtcgccgaccacttcgagggcaaggtcgcgtcgtgggacgtcgtcaacgaggcgttcgccgacggcggcggccgccggcaggactcggcgttccagcagaagctcggcaacggctacatcgagaccgcgttccgggcggcacgtgcggcggacccgaccgccaagctgtgcatcaacgactacaacgtcgagggcatcaacgcgaagagcaactcgctctacgacctcgtcaaggacttcaaggcgcgcggcgtcccgctcgactgcgtcgggttccagtcgcacctcatcgtcggccaggtgccgggcgacttccggcagaacctgcaacggttcgcggacctgggcgtggacgtgcgcatcaccgagctcgacatccgcatgcggacgccctccgacgcgaccaagctcgcgacccaggcggccgactacaagaaggtcgtgcaggcctgcatgcaggtgacccgctgccagggcgtgaccgtctggggcatcaccgacaagtactcgtgggtgccggacgtcttcccgggcgagggggccgcgctggtgtgggacgcgagctacgccaagaagccggcctacgccgccgtgatggaggccttcggcgcgagcccgacgccgacgcccaccacgccgaccccgacgcccacgacgccgacgccgaccccgacgtccggtccggccgggtgccaggtgctgtggggcgtcaaccagtggaacaccggcttcaccgcgaacgtcaccgtgaagaacacgtcctccgctccggtcgacggctggacgctcacgttcagcttcccgtccggccagcaggtcacccaggcgtggagctcgacggtcacgcagtccggctcggccgtgacggtccgcaacgccccgtggaacggctcgatcccggcgggcggcaccgcgcagttcggcttcaacggctcgcacacgggcaccaacgccgcgccgacggcgttctcgctcaacggcacgccctgcacggtcggctaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K118023_sequence/1
type: Sequence
persistent identity: BBa K118023 sequence
display id: BBa_K118023_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtccacccgcagaaccgccgcagcgctgctggcggccgcggccgtcgccgtcggcggtctgaccgccctcaccaccaccgccgcgcaggcggctcccggctgccgcgtcgactacgccgtcaccaaccagtggcccggcggcttcggcgccaacgtcacgatcaccaacctcggcgaccccgtctcgtcgtggaagctcgactggacctacaccgcaggccagcggatccagcagctgtggaacggcaccgcgtcgaccaacggcggccaggtctccgtcaccagcctgccctggaacggcagcatcccgaccggcggcacggcgtcgttcgggttcaacggctcgtgggccgggtccaacccgacgccggcgtcgttctcgctcaacggcaccacctgcacgggcaccgtgccgacgaccagccccacgccgaccccgacgccgacgacccccacgccgacgccgaccccgacccccacccccacgccgacggtcacgccgcagccgaccagcggcttctacgtcgacccgacgacgcagggctaccgcgcgtggcaggccgcgtccggcacggacaaggcgctgctcgagaagatcgcgctcaccccgcaggcgtactgggtcggcaactgggccgacgcgtcgcacgcgcaggccgaggtcgccgactacaccggccgcgccgtcgcggccgggaagacgccgatgctcgtcgtctacgcgatcccgggccgcgactgcggctcgcactccggcggtggtgtgtccgagtccgagtacgcgcgctgggtcgacaccgtcgcgcagggcatcaagggcaacccgatcgtgatcctcgagcccgacgcgctcgcgcagctcggcgactgctccggccagggtgaccgcgtcggcttcctcaagtacgccgccaagtcgctcaccctcaagggcgcgcgcgtctacatcgacgcgggccacgcgaagtggctgtcggtcgacacgccggtgaaccgcctcaaccaggtcggcttcgagtacgcggtgggcttcgcgctcaacacgtcgaactaccagacgacggcggacagcaaggcgtacggccagcagatctcgcagcggctgggcggcaagaagttcgtcatcgacacctcgcgcaacggcaacggctcgaacggcgagtggtgcaacccgcgcggccgcgcgctcggcgaacgcccggtcgcggtgaacgacggctccggcctggacgcgctcctgtgggtcaagctgcccggcgagtccgacggcgcgtgcaacggcggcccggccgccggccagtggtggcaggagatcgccctggagatggcgcgcaacgccaggtggtaataa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z