LOCUS       BBa_J16000              2299 bp    DNA     linear       22-Nov-2024
DEFINITION  iGEM tutorial part
ACCESSION   BBa_J16000
VERSION     BBa_J16000.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    1170..1208
                     /label=LVA
     CDS             81..1208
                     /label=lacI-LVA
     misc_feature    81..1208
                     /label=BBa_C0012
     misc_feature    1209..1233
                     /label=Help:Barcodes
     CDS             81..1208
                     /label=BBa_C0012
     stem_loop       1253..1296
                     /label=stem_loop
     misc_feature    1242..1321
                     /label=BBa_B0010
     terminator      1242..1321
                     /label=BBa_B0010
     stem_loop       1337..1356
                     /label=T7 TE
     misc_feature    1363..1363
                     /label=stop
     polyA_site      1357..1370
                     /label=polya
     misc_feature    1330..1370
                     /label=BBa_B0012
     terminator      1330..1370
                     /label=BBa_B0012
     terminator      1242..1370
                     /label=BBa_B0015
     misc_feature    1381..1397
                     /label=lac O1
     promoter        1398..1403
                     /label=-35
     misc_feature    1404..1420
                     /label=lac O1
     promoter        1421..1426
                     /label=-10
     misc_feature    1379..1432
                     /label=BBa_R0011
     promoter        1379..1432
                     /label=BBa_R0011
     misc_feature    63..1433
                     /label=BBa_Q04121
     CDS             1440..2147
                     /label=mCherry
     misc_feature    1791..1791
                     /label=C->T (removing PstI site)
     misc_feature    2148..2156
                     /label=LVA
     misc_feature    1440..2162
                     /label=BBa_J06505
     stem_loop       2182..2225
                     /label=stem_loop
     misc_feature    2171..2250
                     /label=BBa_B0010
     terminator      2171..2250
                     /label=BBa_B0010
     stem_loop       2266..2285
                     /label=T7 TE
     misc_feature    2292..2292
                     /label=stop
     polyA_site      2286..2299
                     /label=polya
     misc_feature    2259..2299
                     /label=BBa_B0012
     terminator      2259..2299
                     /label=BBa_B0012
     terminator      2171..2299
                     /label=BBa_B0015
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atggtgaatg tgaaaccagt aacgttatac gatgtcgcag
      121 agtatgccgg tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc agccacgttt
      181 ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac attcccaacc
      241 gcgtggcaca acaactggcg ggcaaacagt cgttgctgat tggcgttgcc acctccagtc
      301 tggccctgca cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc gatcaactgg
      361 gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg
      421 tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg ctggatgacc
      481 aggatgccat tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt cttgatgtct
      541 ctgaccagac acccatcaac agtattattt tctcccatga agacggtacg cgactgggcg
      601 tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt
      661 ctgtctcggc gcgtctgcgt ctggctggct ggcataaata tctcactcgc aatcaaattc
      721 agccgatagc ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa caaaccatgc
      781 aaatgctgaa tgagggcatc gttcccactg cgatgctggt tgccaacgat cagatggcgc
      841 tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat atctcggtag
      901 tgggatacga cgataccgaa gacagctcat gttatatccc gccgttaacc accatcaaac
      961 aggattttcg cctgctgggg caaaccagcg tggaccgctt gctgcaactc tctcagggcc
     1021 aggcggtgaa gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa accaccctgg
     1081 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     1141 gacaggtttc ccgactggaa agcgggcagg ctgcaaacga cgaaaactac gctttagtag
     1201 cttaataact ctgatagtgc tagtgtagat ctctactaga gccaggcatc aaataaaacg
     1261 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
     1321 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata tactagagaa
     1381 ttgtgagcgg ataacaattg acattgtgag cggataacaa gatactgagc acatactaga
     1441 tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg
     1501 tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc
     1561 gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct
     1621 tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc
     1681 ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg
     1741 tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ttgcaggacg
     1801 gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa
     1861 tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg
     1921 ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg
     1981 aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca
     2041 acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac
     2101 gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagtta gtagcttaat
     2161 aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
     2221 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
     2281 gggcctttct gcgtttata
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