LOCUS       BBa_J16002              1916 bp    DNA     linear       03-May-2024
DEFINITION  iGEM tutorial part (2)
ACCESSION   BBa_J16002
VERSION     BBa_J16002.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     CDS             85..792
                     /label=cI lambda
     misc_feature    793..825
                     /label=LVA
     misc_feature    832..856
                     /label=Help:Barcodes
     CDS             82..831
                     /label=BBa_C0051
     stem_loop       876..919
                     /label=stem_loop
     misc_feature    865..944
                     /label=BBa_B0010
     terminator      865..944
                     /label=BBa_B0010
     stem_loop       960..979
                     /label=T7 TE
     misc_feature    986..986
                     /label=stop
     polyA_site      980..993
                     /label=polya
     misc_feature    953..993
                     /label=BBa_B0012
     terminator      953..993
                     /label=BBa_B0012
     promoter        1039..1044
                     /label=-10
     promoter        1016..1021
                     /label=-35
     misc_feature    1026..1042
                     /label=OR1
     misc_feature    1002..1018
                     /label=OR2
     misc_feature    1002..1050
                     /label=BBa_R0051
     promoter        1002..1050
                     /label=BBa_R0051
     misc_feature    64..1050
                     /label=BBa_Q04510
     CDS             1057..1764
                     /label=mCherry
     misc_feature    1408..1408
                     /label=C->T (removing PstI site)
     misc_feature    1765..1773
                     /label=LVA
     misc_feature    1057..1779
                     /label=BBa_J06505
     stem_loop       1799..1842
                     /label=stem_loop
     misc_feature    1788..1867
                     /label=BBa_B0010
     terminator      1788..1867
                     /label=BBa_B0010
     stem_loop       1883..1902
                     /label=T7 TE
     misc_feature    1909..1909
                     /label=stop
     polyA_site      1903..1916
                     /label=polya
     misc_feature    1876..1916
                     /label=BBa_B0012
     terminator      1876..1916
                     /label=BBa_B0012
     terminator      1788..1916
                     /label=BBa_B0015
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatgagcaca aaaaagaaac cattaacaca agagcagctt
      121 gaggacgcac gtcgccttaa agcaatttat gaaaaaaaga aaaatgaact tggcttatcc
      181 caggaatctg tcgcagacaa gatggggatg gggcagtcag gcgttggtgc tttatttaat
      241 ggcatcaatg cattaaatgc ttataacgcc gcattgcttg caaaaattct caaagttagc
      301 gttgaagaat ttagcccttc aatcgccaga gaaatctacg agatgtatga agcggttagt
      361 atgcagccgt cacttagaag tgagtatgag taccctgttt tttctcatgt tcaggcaggg
      421 atgttctcac ctgagcttag aacctttacc aaaggtgatg cggagagatg ggtaagcaca
      481 accaaaaaag ccagtgattc tgcattctgg cttgaggttg aaggtaattc catgaccgca
      541 ccaacaggct ccaagccaag ctttcctgac ggaatgttaa ttctcgttga ccctgagcag
      601 gctgttgagc caggtgattt ctgcatagcc agacttgggg gtgatgagtt taccttcaag
      661 aaactgatca gggatagcgg tcaggtgttt ttacaaccac taaacccaca gtacccaatg
      721 atcccatgca atgagagttg ttccgttgtg gggaaagtta tcgctagtca gtggcctgaa
      781 gagacgtttg gcgctgcaaa cgacgaaaac tacgctttag tagcttaata acgctgatag
      841 tgctagtgta gatcgctact agagccaggc atcaaataaa acgaaaggct cagtcgaaag
      901 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
      961 gctcaccttc gggtgggcct ttctgcgttt atatactaga gtaacaccgt gcgtgttgac
     1021 tattttacct ctggcggtga taatggttgc tactagatgg tgagcaaggg cgaggaggat
     1081 aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac
     1141 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc
     1201 gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct
     1261 cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg
     1321 aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc
     1381 gtggtgaccg tgacccagga ctcctccttg caggacggcg agttcatcta caaggtgaag
     1441 ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg
     1501 gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag
     1561 aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc
     1621 aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc
     1681 cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc
     1741 ggcggcatgg acgagctgta caagttagta gcttaataat actagagcca ggcatcaaat
     1801 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
     1861 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata
//