BBa_J16103
1
BBa_J16103
SPOM - Super Powers Output Module
2006-07-23T11:00:00Z
2015-08-31T04:08:33Z
Basic Ribosomal and Terminator Parts flank the SupR gene take from Clark Kent
When given a PoPs input will give the host Super Powers beyound their wildless dreams
false
false
_1_
0
740
1
Not in stock
false
Use with Caution
false
James Brown
component1890736
1
BBa_B0030
component1890740
1
BBa_B0010
component1890739
1
BBa_J16100
component1890742
1
BBa_B0012
annotation1890739
1
BBa_J16100
range1890739
1
22
116
annotation1890736
1
BBa_B0030
range1890736
1
1
15
annotation1890742
1
BBa_B0012
range1890742
1
213
253
annotation1890740
1
BBa_B0010
range1890740
1
125
204
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1690
1
polya
range1690
1
28
41
annotation1687
1
stop
range1687
1
34
34
annotation1686
1
T7 TE
range1686
1
8
27
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation1701
1
RBS-1\Strong
range1701
1
1
15
annotation7025
1
BBa_B0030
range7025
1
1
15
annotation1702
1
RBS
range1702
1
8
12
BBa_J16100
1
BBa_J16100
SupR Gene
2006-07-23T11:00:00Z
2015-08-31T04:08:33Z
Acquired from a DNA sample taken from Clark Kent's coffee mug at the Daily Planet
The SupR gene encodes the operate super proteins that give Superman all this special powers. Without this gene, he'd just be plain old Clark Kent.
The protein Krypton binds to a section of this coding region (see features map), blocking he polymerase and inhibiting transcription of the gene, effectively leaving Superman powerless.
false
false
_1_
0
740
1
Not in stock
false
It had one BB restriction site within its sequence - this was removed
false
James Brown
annotation1890734
1
Krypton Bnding Site - inhibits
range1890734
1
20
30
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_J16103_sequence
1
attaaagaggagaaatactagatgatgcgttattgcgtagatgcggattcggatcacacagtgagattcgctatcggggatgctaatctatatcgatgccctctctcgatagcatgtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_J16100_sequence
1
atgatgcgttattgcgtagatgcggattcggatcacacagtgagattcgctatcggggatgctaatctatatcgatgccctctctcgatagcatg
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z