LOCUS BBa_K327001 2331 bp DNA linear 26-Dec-2024 DEFINITION RBS+CI+RBS+RFP+F1610 ACCESSION BBa_K327001 VERSION BBa_K327001.1 FEATURES Location/Qualifiers misc_feature 5..8 /label=conserved RBS 1..12 /label=BBa_B0034 CDS 22..729 /label=cI lambda misc_feature 730..762 /label=LVA misc_feature 769..793 /label=Help:Barcodes CDS 19..793 /label=BBa_C0051 misc_feature 806..809 /label=conserved RBS 802..813 /label=BBa_B0034 CDS 820..1494 /label=mrfp1 misc_feature 1501..1525 /label=Help:Barcodes CDS 820..1525 /label=BBa_E1010 misc_feature 1538..1541 /label=conserved RBS 1534..1545 /label=BBa_B0034 misc_feature 2131..2162 /label=LVA CDS 1552..2130 /label=luxI misc_feature 1552..2169 /label=BBa_C0061 misc_feature 2170..2194 /label=Help:Barcodes CDS 1552..2169 /label=BBa_C0061 stem_loop 2214..2257 /label=stem_loop misc_feature 2203..2282 /label=BBa_B0010 terminator 2203..2282 /label=BBa_B0010 stem_loop 2298..2317 /label=T7 TE misc_feature 2324..2324 /label=stop polyA_site 2318..2331 /label=polya misc_feature 2291..2331 /label=BBa_B0012 terminator 2291..2331 /label=BBa_B0012 misc_feature 1534..2331 /label=BBa_F1610 ORIGIN 1 aaagaggaga aatactagat gagcacaaaa aagaaaccat taacacaaga gcagcttgag 61 gacgcacgtc gccttaaagc aatttatgaa aaaaagaaaa atgaacttgg cttatcccag 121 gaatctgtcg cagacaagat ggggatgggg cagtcaggcg ttggtgcttt atttaatggc 181 atcaatgcat taaatgctta taacgccgca ttgcttgcaa aaattctcaa agttagcgtt 241 gaagaattta gcccttcaat cgccagagaa atctacgaga tgtatgaagc ggttagtatg 301 cagccgtcac ttagaagtga gtatgagtac cctgtttttt ctcatgttca ggcagggatg 361 ttctcacctg agcttagaac ctttaccaaa ggtgatgcgg agagatgggt aagcacaacc 421 aaaaaagcca gtgattctgc attctggctt gaggttgaag gtaattccat gaccgcacca 481 acaggctcca agccaagctt tcctgacgga atgttaattc tcgttgaccc tgagcaggct 541 gttgagccag gtgatttctg catagccaga cttgggggtg atgagtttac cttcaagaaa 601 ctgatcaggg atagcggtca ggtgttttta caaccactaa acccacagta cccaatgatc 661 ccatgcaatg agagttgttc cgttgtgggg aaagttatcg ctagtcagtg gcctgaagag 721 acgtttggcg ctgcaaacga cgaaaactac gctttagtag cttaataacg ctgatagtgc 781 tagtgtagat cgctactaga gaaagaggag aaatactaga tggcttcctc cgaagacgtt 841 atcaaagagt tcatgcgttt caaagttcgt atggaaggtt ccgttaacgg tcacgagttc 901 gaaatcgaag gtgaaggtga aggtcgtccg tacgaaggta cccagaccgc taaactgaaa 961 gttaccaaag gtggtccgct gccgttcgct tgggacatcc tgtccccgca gttccagtac 1021 ggttccaaag cttacgttaa acacccggct gacatcccgg actacctgaa actgtccttc 1081 ccggaaggtt tcaaatggga acgtgttatg aacttcgaag acggtggtgt tgttaccgtt 1141 acccaggact cctccctgca agacggtgag ttcatctaca aagttaaact gcgtggtacc 1201 aacttcccgt ccgacggtcc ggttatgcag aaaaaaacca tgggttggga agcttccacc 1261 gaacgtatgt acccggaaga cggtgctctg aaaggtgaaa tcaaaatgcg tctgaaactg 1321 aaagacggtg gtcactacga cgctgaagtt aaaaccacct acatggctaa aaaaccggtt 1381 cagctgccgg gtgcttacaa aaccgacatc aaactggaca tcacctccca caacgaagac 1441 tacaccatcg ttgaacagta cgaacgtgct gaaggtcgtc actccaccgg tgcttaataa 1501 cgctgatagt gctagtgtag atcgctacta gagaaagagg agaaatacta gatgactata 1561 atgataaaaa aatcggattt tttggcaatt ccatcggagg agtataaagg tattctaagt 1621 cttcgttatc aagtgtttaa gcaaagactt gagtgggact tagttgtaga aaataacctt 1681 gaatcagatg agtatgataa ctcaaatgca gaatatattt atgcttgtga tgatactgaa 1741 aatgtaagtg gatgctggcg tttattacct acaacaggtg attatatgct gaaaagtgtt 1801 tttcctgaat tgcttggtca acagagtgct cccaaagatc ctaatatagt cgaattaagt 1861 cgttttgctg taggtaaaaa tagctcaaag ataaataact ctgctagtga aattacaatg 1921 aaactatttg aagctatata taaacacgct gttagtcaag gtattacaga atatgtaaca 1981 gtaacatcaa cagcaataga gcgattttta aagcgtatta aagttccttg tcatcgtatt 2041 ggagacaaag aaattcatgt attaggtgat actaaatcgg ttgtattgtc tatgcctatt 2101 aatgaacagt ttaaaaaagc agtcttaaat gctgcaaacg acgaaaacta cgctttagta 2161 gcttaataac tctgatagtg ctagtgtaga tctctactag agccaggcat caaataaaac 2221 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc 2281 tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a // LOCUS BBa_J162001 2331 bp DNA linear 26-Dec-2024 DEFINITION RBS cl RBS RFP RBS 3OC6HSL Double Terminator ACCESSION BBa_J162001 VERSION BBa_J162001.1 FEATURES Location/Qualifiers misc_feature 5..8 /label=conserved RBS 1..12 /label=BBa_B0034 CDS 22..729 /label=cI lambda misc_feature 730..762 /label=LVA misc_feature 769..793 /label=Help:Barcodes CDS 19..768 /label=BBa_C0051 misc_feature 806..809 /label=conserved RBS 802..813 /label=BBa_B0034 CDS 820..1494 /label=mrfp1 misc_feature 1501..1525 /label=Help:Barcodes CDS 820..1500 /label=BBa_E1010 misc_feature 1538..1541 /label=conserved RBS 1534..1545 /label=BBa_B0034 misc_feature 2131..2162 /label=LVA CDS 1552..2130 /label=luxI misc_feature 1552..2169 /label=BBa_C0061 misc_feature 2170..2194 /label=Help:Barcodes CDS 1552..2169 /label=BBa_C0061 stem_loop 2214..2257 /label=stem_loop misc_feature 2203..2282 /label=BBa_B0010 terminator 2203..2282 /label=BBa_B0010 stem_loop 2298..2317 /label=T7 TE misc_feature 2324..2324 /label=stop polyA_site 2318..2331 /label=polya misc_feature 2291..2331 /label=BBa_B0012 terminator 2291..2331 /label=BBa_B0012 misc_feature 1534..2331 /label=BBa_F1610 ORIGIN 1 aaagaggaga aatactagat gagcacaaaa aagaaaccat taacacaaga gcagcttgag 61 gacgcacgtc gccttaaagc aatttatgaa aaaaagaaaa atgaacttgg cttatcccag 121 gaatctgtcg cagacaagat ggggatgggg cagtcaggcg ttggtgcttt atttaatggc 181 atcaatgcat taaatgctta taacgccgca ttgcttgcaa aaattctcaa agttagcgtt 241 gaagaattta gcccttcaat cgccagagaa atctacgaga tgtatgaagc ggttagtatg 301 cagccgtcac ttagaagtga gtatgagtac cctgtttttt ctcatgttca ggcagggatg 361 ttctcacctg agcttagaac ctttaccaaa ggtgatgcgg agagatgggt aagcacaacc 421 aaaaaagcca gtgattctgc attctggctt gaggttgaag gtaattccat gaccgcacca 481 acaggctcca agccaagctt tcctgacgga atgttaattc tcgttgaccc tgagcaggct 541 gttgagccag gtgatttctg catagccaga cttgggggtg atgagtttac cttcaagaaa 601 ctgatcaggg atagcggtca ggtgttttta caaccactaa acccacagta cccaatgatc 661 ccatgcaatg agagttgttc cgttgtgggg aaagttatcg ctagtcagtg gcctgaagag 721 acgtttggcg ctgcaaacga cgaaaactac gctttagtag cttaataacg ctgatagtgc 781 tagtgtagat cgctactaga gaaagaggag aaatactaga tggcttcctc cgaagacgtt 841 atcaaagagt tcatgcgttt caaagttcgt atggaaggtt ccgttaacgg tcacgagttc 901 gaaatcgaag gtgaaggtga aggtcgtccg tacgaaggta cccagaccgc taaactgaaa 961 gttaccaaag gtggtccgct gccgttcgct tgggacatcc tgtccccgca gttccagtac 1021 ggttccaaag cttacgttaa acacccggct gacatcccgg actacctgaa actgtccttc 1081 ccggaaggtt tcaaatggga acgtgttatg aacttcgaag acggtggtgt tgttaccgtt 1141 acccaggact cctccctgca agacggtgag ttcatctaca aagttaaact gcgtggtacc 1201 aacttcccgt ccgacggtcc ggttatgcag aaaaaaacca tgggttggga agcttccacc 1261 gaacgtatgt acccggaaga cggtgctctg aaaggtgaaa tcaaaatgcg tctgaaactg 1321 aaagacggtg gtcactacga cgctgaagtt aaaaccacct acatggctaa aaaaccggtt 1381 cagctgccgg gtgcttacaa aaccgacatc aaactggaca tcacctccca caacgaagac 1441 tacaccatcg ttgaacagta cgaacgtgct gaaggtcgtc actccaccgg tgcttaataa 1501 cgctgatagt gctagtgtag atcgctacta gagaaagagg agaaatacta gatgactata 1561 atgataaaaa aatcggattt tttggcaatt ccatcggagg agtataaagg tattctaagt 1621 cttcgttatc aagtgtttaa gcaaagactt gagtgggact tagttgtaga aaataacctt 1681 gaatcagatg agtatgataa ctcaaatgca gaatatattt atgcttgtga tgatactgaa 1741 aatgtaagtg gatgctggcg tttattacct acaacaggtg attatatgct gaaaagtgtt 1801 tttcctgaat tgcttggtca acagagtgct cccaaagatc ctaatatagt cgaattaagt 1861 cgttttgctg taggtaaaaa tagctcaaag ataaataact ctgctagtga aattacaatg 1921 aaactatttg aagctatata taaacacgct gttagtcaag gtattacaga atatgtaaca 1981 gtaacatcaa cagcaataga gcgattttta aagcgtatta aagttccttg tcatcgtatt 2041 ggagacaaag aaattcatgt attaggtgat actaaatcgg ttgtattgtc tatgcctatt 2101 aatgaacagt ttaaaaaagc agtcttaaat gctgcaaacg acgaaaacta cgctttagta 2161 gcttaataac tctgatagtg ctagtgtaga tctctactag agccaggcat caaataaaac 2221 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc 2281 tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a //