LOCUS       BBa_J162003             2991 bp    DNA     linear       22-Nov-2024
DEFINITION  J23101_J61002
ACCESSION   BBa_J162003
VERSION     BBa_J162003.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23101
     promoter        2771..2799
                     /label=beta-lactamase (ampR, bla) promoter
     CDS             70..750
                     /label=mRFP
     misc_feature    784..912
                     /label=BBa_B0015
     misc_feature    1092..1774
                     /label=ColE1 origin
     CDS             1869..2729
                     /label=beta-lactamase ampicillin resistance marker
     stem_loop       795..838
                     /label=rrnB terminator
     misc_feature    1095..1714
                     /label=pBR322 origin
     misc_feature    2970..2991
                     /label=BioBrick prefix
     misc_feature    45..50
                     /label=SpeI
     misc_feature    928..933
                     /label=PstI
     misc_feature    921..928
                     /label=NotI
     RBS             52..63
                     /label=RBS
     misc_feature    52..63
                     /label=BBa_B0034 RBS
     primer_bind     991..1010
                     /label=Verification reverse (VR) primer binding site
     primer_bind     2852..2871
                     /label=Verification forward (VF2) primer binding site
     misc_feature    2984..2984
                     /label=Ambiguity; should be a T
     misc_feature    44..2991
                     /label=BBa_J61002
ORIGIN
        1 tttacagcta gctcagtcct aggtattatg ctagctacta gagtactagt gaaagaggag
       61 aaatactaga tggcttcctc cgaagacgtt atcaaagagt tcatgcgttt caaagttcgt
      121 atggaaggtt ccgttaacgg tcacgagttc gaaatcgaag gtgaaggtga aggtcgtccg
      181 tacgaaggta cccagaccgc taaactgaaa gttaccaaag gtggtccgct gccgttcgct
      241 tgggacatcc tgtccccgca gttccagtac ggttccaaag cttacgttaa acacccggct
      301 gacatcccgg actacctgaa actgtccttc ccggaaggtt tcaaatggga acgtgttatg
      361 aacttcgaag acggtggtgt tgttaccgtt acccaggact cctccctgca agacggtgag
      421 ttcatctaca aagttaaact gcgtggtacc aacttcccgt ccgacggtcc ggttatgcag
      481 aaaaaaacca tgggttggga agcttccacc gaacgtatgt acccggaaga cggtgctctg
      541 aaaggtgaaa tcaaaatgcg tctgaaactg aaagacggtg gtcactacga cgctgaagtt
      601 aaaaccacct acatggctaa aaaaccggtt cagctgccgg gtgcttacaa aaccgacatc
      661 aaactggaca tcacctccca caacgaagac tacaccatcg ttgaacagta cgaacgtgct
      721 gaaggtcgtc actccaccgg tgcttaataa cgctgatagt gctagtgtag atcgctacta
      781 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
      841 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt
      901 tctgcgttta tatactagaa gcggccgctg caggcttcct cgctcactga ctcgctgcgc
      961 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     1021 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     1081 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     1141 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     1201 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     1261 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     1321 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     1381 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     1441 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     1501 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
     1561 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     1621 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     1681 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     1741 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     1801 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     1861 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     1921 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     1981 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     2041 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     2101 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     2161 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     2221 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     2281 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     2341 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     2401 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     2461 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     2521 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     2581 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     2641 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     2701 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     2761 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     2821 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
     2881 atcatgacat taacctataa aaataggcgt atcacgaggc agaatttcag ataaaaaaaa
     2941 tccttagctt tcgctaagga tgatttctgg aattcgcggc cgcatctaga g
//