##gff-version 3
##sequence-region BBa_J162003 1 2991
BBa_J162003	.	promoter	1	35	.	+	0	ID=BBa_J23101;Name=BBa_J23101
BBa_J162003	.	plasmid_vector	44	2991	.	+	0	ID=BBa_J61002;Name=BBa_J61002
BBa_J162003	.	sequence_feature	928	933	.	+	0	ID=annotation1975717;Name=PstI;Parent=BBa_J61002
BBa_J162003	.	sequence_feature	1095	1714	.	+	0	ID=annotation1975714;Name=pBR322 origin;Parent=BBa_J61002
BBa_J162003	.	sequence_feature	921	928	.	+	0	ID=annotation1975718;Name=NotI;Parent=BBa_J61002
BBa_J162003	.	CDS	1869	2729	.	+	0	ID=annotation1892276;Name=beta-lactamase ampicillin resistance marker;Parent=BBa_J61002
BBa_J162003	.	primer_binding_site	991	1010	.	+	0	ID=annotation1975768;Name=Verification reverse (VR) primer binding site;Parent=BBa_J61002
BBa_J162003	.	sequence_alteration	2984	2984	.	+	0	ID=annotation1975921;Name=Ambiguity; should be a T;Parent=BBa_J61002
BBa_J162003	.	CDS	70	750	.	+	0	ID=annotation1892273;Name=mRFP;Parent=BBa_J61002
BBa_J162003	.	sequence_feature	1092	1774	.	+	0	ID=annotation1892275;Name=ColE1 origin;Parent=BBa_J61002
BBa_J162003	.	sequence_feature	2970	2991	.	+	0	ID=annotation1975715;Name=BioBrick prefix;Parent=BBa_J61002
BBa_J162003	.	promoter	2771	2799	.	+	0	ID=annotation1892272;Name=beta-lactamase (ampR, bla) promoter;Parent=BBa_J61002
BBa_J162003	.	engineered_region	784	912	.	+	0	ID=annotation1892274;Name=BBa_B0015;Parent=BBa_J61002
BBa_J162003	.	stem_loop	795	838	.	+	0	ID=annotation1975713;Name=rrnB terminator;Parent=BBa_J61002
BBa_J162003	.	ribosome_entry_site	52	63	.	+	0	ID=annotation1975766;Name=RBS;Parent=BBa_J61002
BBa_J162003	.	primer_binding_site	2852	2871	.	+	0	ID=annotation1975769;Name=Verification forward (VF2) primer binding site;Parent=BBa_J61002
BBa_J162003	.	engineered_region	52	63	.	+	0	ID=annotation1975767;Name=BBa_B0034 RBS;Parent=BBa_J61002
BBa_J162003	.	sequence_feature	45	50	.	+	0	ID=annotation1975716;Name=SpeI;Parent=BBa_J61002
>BBa_J162003
tttacagctagctcagtcctaggtattatgctagctactagagtactagtgaaagaggagaaatactagatggcttcct
ccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcga
aggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttc
gcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacc
tgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttaccca
ggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggtt
atgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatca
aaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttca
gctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtac
gaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggca
tcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactag
agtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaagcggccgctgcaggcttcctcgctcact
gactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaat
caggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggc
gtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacagga
ctataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacc
tgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgt
tcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgag
tccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcg
gtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaa
gccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtt
tgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagt
ggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaa
atgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacct
atctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggct
taccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagcc
agccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagct
agagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgt
ttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggt
tagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcat
aattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagt
gtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgct
catcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccact
cgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccg
caaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatca
gggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccc
cgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcaga
atttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgcggccgcatctagag