LOCUS       BBa_J176043             2642 bp    DNA     linear       22-Dec-2024
DEFINITION  Dox-inducible Pc Silencer
ACCESSION   BBa_J176043
VERSION     BBa_J176043.1  
FEATURES             Location/Qualifiers
     misc_feature    573..579
                     /label=TATA box
     misc_feature    589..628
                     /label=TetO x2
     promoter        1..727
                     /label=BBa_J176017
     misc_feature    728..733
                     /label=BBa_J176041
     RBS             734..751
                     /label=BBa_J176012
     misc_feature    752..757
                     /label=BBa_J176041
     misc_feature    758..1216
                     /label=BBa_J176020
     misc_feature    1217..1222
                     /label=BBa_J176041
     misc_feature    1223..2623
                     /label=BBa_J176023
     misc_feature    1..2623
                     /label=BBa_S04681
     misc_feature    2624..2629
                     /label=BBa_J176041
     terminator      2630..2642
                     /label=BBa_J176016
ORIGIN
        1 gttgacattg attattggct agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
      421 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
      481 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
      541 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctctc cctatcagtg
      601 atagagatct ccctatcagt gatagagatc gtcgacgagc tcgtttagtg aaccgtcaga
      661 tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca
      721 gcctccgact agacccgccg ccaccatgga gactagaatg aagctactgt cttctatcga
      781 acaagcatgc gatatttgcc gacttaaaaa gctcaagtgc tccaaagaaa aaccgaagtg
      841 cgccaagtgt ctgaagaaca actgggagtg tcgctactct cccaaaacca aaaggtctcc
      901 gctgactagg gcacatctga cagaagtgga atcaaggcta gaaagactgg aacagctatt
      961 tctactgatt tttcctcgag aagaccttga catgattttg aaaatggatt ctttacagga
     1021 tataaaagca ttgttaacag gattatttgt acaagataat gtgaataaag atgccgtcac
     1081 agatagattg gcttcagtgg agactgatat gcctctaaca ttgagacagc atagaataag
     1141 tgcgacatca tcatcggaag agagtagtaa caaaggtcaa agacagttga ctgtatcgcc
     1201 ggaatttccg gggatcacta gaatgtccga gagggaagtg tcgactgcgc cggcgggaac
     1261 agacatgcct gcggccaaga agcagaagct gagcagtgac gagaacagca atccagacct
     1321 ctctggagac gagaatgatg acgctgtcag tatagaaagt ggtacaaaca ctgaacgccc
     1381 tgatacacct acaaacacgc caaatgcacc tggaaggaaa agttggggaa agggaaaatg
     1441 gaagtcaaag aaatgcaaat attctttcaa atgtgtaaat agtctcaagg aagatcataa
     1501 ccaaccattg tttggagttc agtttaactg gcacagtaaa gaaggagatc cattagtgtt
     1561 tgcaactgta ggaagcaaca gagttacctt gtatgaatgt cattcacaag gagaaatccg
     1621 gttgttgcaa tcttacgtgg atgctgatgc tgatgaaaac ttttacactt gtgcatggac
     1681 ctatgatagc aatacgagcc atcctctgct ggctgtagct ggatcaagag gcataattag
     1741 gataataaat cctataacaa tgcagtgtat aaagcactat gttggccatg gaaatgctat
     1801 caatgagctg aaattccatc caagagatcc aaatcttctc ctgtcagtaa gtaaagatca
     1861 tgctttacga ttatggaata tccagacgga cactctggtg gcaatatttg gaggcgtaga
     1921 agggcacaga gatgaagttc taagtgctga ttatgatctt ttgggtgaaa aaataatgtc
     1981 ctgtggtatg gatcattctc ttaaactttg gaggatcaat tcaaagagaa tgatgaatgc
     2041 aattaaggaa tcttatgatt ataatccaaa taaaactaac aggccattta tttctcagaa
     2101 aatccatttt cctgattttt ctaccagaga catacatagg aattatgttg attgtgtgcg
     2161 atggttaggc gatttgatac tttctaagag tggccgtgcc attttacatt cccaccagca
     2221 atgtatgaga gatccagtgt ctccgaatct tcgccagcat ttgtcttgtg aaaatgccat
     2281 tgtgtgctgg aaacctggca agatggaaga tgatatagat aaaattaaac ccagtgaatc
     2341 taatgtgact attcttgggc gatttgatta cagccagtgt gacatttggt acatgaggtt
     2401 ttctatggat ttctggcaaa agatgcttgc attgggcaat caagttggca aactttatgt
     2461 ttgggattta gaagtagaag atcctcataa agccaaatgt acaacactga ctcatcataa
     2521 atgtggtgct gctattcgac aaaccagttt tagcagggat agcagcattc ttatagctgt
     2581 ttgtgatgat gccagtattt ggcgctggga tcgacttcga ttgactagag cgtaaagctg
     2641 ag
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