LOCUS       BBa_J176044             2177 bp    DNA     linear       22-Nov-2024
DEFINITION  Pc silencer, no DB neg. control
ACCESSION   BBa_J176044
VERSION     BBa_J176044.1  
FEATURES             Location/Qualifiers
     misc_feature    573..579
                     /label=TATA box
     misc_feature    589..628
                     /label=TetO x2
     promoter        1..727
                     /label=BBa_J176017
     misc_feature    728..733
                     /label=BBa_J176041
     RBS             734..751
                     /label=BBa_J176012
     misc_feature    752..757
                     /label=BBa_J176041
     misc_feature    758..2158
                     /label=BBa_J176023
     misc_feature    1..2158
                     /label=BBa_S04682
     misc_feature    2159..2164
                     /label=BBa_J176041
     terminator      2165..2177
                     /label=BBa_J176016
ORIGIN
        1 gttgacattg attattggct agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
      421 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
      481 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
      541 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctctc cctatcagtg
      601 atagagatct ccctatcagt gatagagatc gtcgacgagc tcgtttagtg aaccgtcaga
      661 tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca
      721 gcctccgact agacccgccg ccaccatgga gactagaatg tccgagaggg aagtgtcgac
      781 tgcgccggcg ggaacagaca tgcctgcggc caagaagcag aagctgagca gtgacgagaa
      841 cagcaatcca gacctctctg gagacgagaa tgatgacgct gtcagtatag aaagtggtac
      901 aaacactgaa cgccctgata cacctacaaa cacgccaaat gcacctggaa ggaaaagttg
      961 gggaaaggga aaatggaagt caaagaaatg caaatattct ttcaaatgtg taaatagtct
     1021 caaggaagat cataaccaac cattgtttgg agttcagttt aactggcaca gtaaagaagg
     1081 agatccatta gtgtttgcaa ctgtaggaag caacagagtt accttgtatg aatgtcattc
     1141 acaaggagaa atccggttgt tgcaatctta cgtggatgct gatgctgatg aaaactttta
     1201 cacttgtgca tggacctatg atagcaatac gagccatcct ctgctggctg tagctggatc
     1261 aagaggcata attaggataa taaatcctat aacaatgcag tgtataaagc actatgttgg
     1321 ccatggaaat gctatcaatg agctgaaatt ccatccaaga gatccaaatc ttctcctgtc
     1381 agtaagtaaa gatcatgctt tacgattatg gaatatccag acggacactc tggtggcaat
     1441 atttggaggc gtagaagggc acagagatga agttctaagt gctgattatg atcttttggg
     1501 tgaaaaaata atgtcctgtg gtatggatca ttctcttaaa ctttggagga tcaattcaaa
     1561 gagaatgatg aatgcaatta aggaatctta tgattataat ccaaataaaa ctaacaggcc
     1621 atttatttct cagaaaatcc attttcctga tttttctacc agagacatac ataggaatta
     1681 tgttgattgt gtgcgatggt taggcgattt gatactttct aagagtggcc gtgccatttt
     1741 acattcccac cagcaatgta tgagagatcc agtgtctccg aatcttcgcc agcatttgtc
     1801 ttgtgaaaat gccattgtgt gctggaaacc tggcaagatg gaagatgata tagataaaat
     1861 taaacccagt gaatctaatg tgactattct tgggcgattt gattacagcc agtgtgacat
     1921 ttggtacatg aggttttcta tggatttctg gcaaaagatg cttgcattgg gcaatcaagt
     1981 tggcaaactt tatgtttggg atttagaagt agaagatcct cataaagcca aatgtacaac
     2041 actgactcat cataaatgtg gtgctgctat tcgacaaacc agttttagca gggatagcag
     2101 cattcttata gctgtttgtg atgatgccag tatttggcgc tgggatcgac ttcgattgac
     2161 tagagcgtaa agctgag
//