BBa_J176046
type: ComponentDefinition
persistent identity: BBa J176046
display id: BBa_J176046
version: 1
was generated by: iGEM to SBOL conversion
description: Luc miRNA RFP-reporter 1
created: 2011-10-07T11:00:00Z
modified: 2015-08-31T04:08:34Z
mutable provenance: TBA
owned by: james
owned by: myers
mutable description: Luc miRNA RFP-reporter 1
discontinued: false
dominant: false
group u list: _863_
m user id: 0
owner id: 10865
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: TBA
star: false
creator: Brady Laughlin
type: DnaRegion
role: SO:0000110
role: partType/Temporary
component: BBa_S04699
component: BBa_S04691
component: BBa_J176041
sequence annotation: BBa_S04699
sequence annotation: BBa_J176041
sequence annotation: BBa_S04691
sequence: https://synbiohub.org/public/igem/BBa_J176046_sequence/1

BBa_S04699
type: Component
persistent identity: component2157106
display id: component2157106
version: 1
top level: BBa_J176046
definition: BBa_S04699
access: public

BBa_S04691
type: Component
persistent identity: component2157116
display id: component2157116
version: 1
top level: BBa_J176046
definition: BBa_S04691
access: public

BBa_J176041
type: Component
persistent identity: component2157107
display id: component2157107
version: 1
top level: BBa_J176046
definition: scar
access: public

BBa_S04699
type: SequenceAnnotation
persistent identity: annotation2157106
display id: annotation2157106
version: 1
top level: BBa_J176046
location: https://synbiohub.org/public/igem/BBa_J176046/annotation2157106/range2157106/1
component: BBa_S04699

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persistent identity: range2157106
display id: range2157106
version: 1
top level: BBa_J176046
direction: direction/reverse
start: 1
end: 3557
orientation: inline

BBa_J176041
type: SequenceAnnotation
persistent identity: annotation2157107
display id: annotation2157107
version: 1
top level: BBa_J176046
location: https://synbiohub.org/public/igem/BBa_J176046/annotation2157107/range2157107/1
component: BBa_J176041

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persistent identity: range2157107
display id: range2157107
version: 1
top level: BBa_J176046
direction: direction/reverse
start: 3558
end: 3563
orientation: inline

BBa_S04691
type: SequenceAnnotation
persistent identity: annotation2157116
display id: annotation2157116
version: 1
top level: BBa_J176046
location: https://synbiohub.org/public/igem/BBa_J176046/annotation2157116/range2157116/1
component: BBa_S04691

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type: Range
persistent identity: range2157116
display id: range2157116
version: 1
top level: BBa_J176046
direction: direction/reverse
start: 3564
end: 4916
orientation: inline

scar
type: ComponentDefinition
persistent identity: BBa J176041
display id: BBa_J176041
version: 1
was generated by: iGEM to SBOL conversion
description: Silver scar
created: 2011-10-04T11:00:00Z
modified: 2015-08-31T04:08:34Z
mutable provenance: This sequence is generated when BioBricks are assembled via assembly standard 23 (Silver standard).
owned by: james
owned by: myers
mutable description: Six base pair scar between parts that have been assembled using BioBrick assembly standard 23 (Silver standard).
discontinued: false
dominant: false
experience: experience/None
group u list: _863_
m user id: 0
owner id: 1144
owning group id: 61
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The six base pair scar allows protein modules to be assembled in frame. This scar retains the triplet pattern for proper translation of the mRNA. Two extra amino acids, Thr and Arg, are introduced into the protein fusion, but in our hands, this does not disrupt the function of our synthetic mammalian parts.
star: false
creator: Karmella Haynes
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_J176041_sequence/1

BBa_S04691
type: ComponentDefinition
persistent identity: BBa S04691
display id: BBa_S04691
version: 1
was generated by: iGEM to SBOL conversion
description: J176025:J176009
created: 2011-10-06T11:00:00Z
modified: 2015-05-08T01:14:44Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 10865
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Brady Laughlin
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_J176025
component: BBa_J176042
component: BBa_J176041
component: BBa_J176021
sequence annotation: BBa_J176025
sequence annotation: BBa_J176041
sequence annotation: BBa_J176042
sequence annotation: BBa_J176021
sequence: https://synbiohub.org/public/igem/BBa_S04691_sequence/1

BBa_J176025
type: Component
persistent identity: component2156718
display id: component2156718
version: 1
top level: BBa_S04691
definition: miRts luc
access: public

BBa_J176042
type: Component
persistent identity: component2156721
display id: component2156721
version: 1
top level: BBa_S04691
definition: polyA
access: public

BBa_J176041
type: Component
persistent identity: component2156719
display id: component2156719
version: 1
top level: BBa_S04691
definition: scar
access: public

BBa_J176021
type: Component
persistent identity: component2156723
display id: component2156723
version: 1
top level: BBa_S04691
definition: HS4
access: public

BBa_J176041
type: Component
persistent identity: component2156722
display id: component2156722
version: 1
top level: BBa_S04691
definition: scar
access: public

BBa_J176021
type: Component
persistent identity: component2156725
display id: component2156725
version: 1
top level: BBa_S04691
definition: HS4
access: public

BBa_J176041
type: Component
persistent identity: component2156724
display id: component2156724
version: 1
top level: BBa_S04691
definition: scar
access: public

BBa_J176025
type: SequenceAnnotation
persistent identity: annotation2156718
display id: annotation2156718
version: 1
top level: BBa_S04691
location: https://synbiohub.org/public/igem/BBa_S04691/annotation2156718/range2156718/1
component: BBa_J176025

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persistent identity: range2156718
display id: range2156718
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direction: direction/reverse
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orientation: inline

BBa_J176041
type: SequenceAnnotation
persistent identity: annotation2156719
display id: annotation2156719
version: 1
top level: BBa_S04691
location: https://synbiohub.org/public/igem/BBa_S04691/annotation2156719/range2156719/1
component: BBa_J176041

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type: Range
persistent identity: range2156719
display id: range2156719
version: 1
top level: BBa_S04691
direction: direction/reverse
start: 188
end: 193
orientation: inline

BBa_J176042
type: SequenceAnnotation
persistent identity: annotation2156721
display id: annotation2156721
version: 1
top level: BBa_S04691
location: https://synbiohub.org/public/igem/BBa_S04691/annotation2156721/range2156721/1
component: BBa_J176042

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type: Range
persistent identity: range2156721
display id: range2156721
version: 1
top level: BBa_S04691
direction: direction/reverse
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end: 661
orientation: inline

BBa_J176041
type: SequenceAnnotation
persistent identity: annotation2156722
display id: annotation2156722
version: 1
top level: BBa_S04691
location: https://synbiohub.org/public/igem/BBa_S04691/annotation2156722/range2156722/1
component: BBa_J176041

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type: Range
persistent identity: range2156722
display id: range2156722
version: 1
top level: BBa_S04691
direction: direction/reverse
start: 662
end: 667
orientation: inline

BBa_J176021
type: SequenceAnnotation
persistent identity: annotation2156723
display id: annotation2156723
version: 1
top level: BBa_S04691
location: https://synbiohub.org/public/igem/BBa_S04691/annotation2156723/range2156723/1
component: BBa_J176021

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type: Range
persistent identity: range2156723
display id: range2156723
version: 1
top level: BBa_S04691
direction: direction/reverse
start: 668
end: 1007
orientation: inline

BBa_J176041
type: SequenceAnnotation
persistent identity: annotation2156724
display id: annotation2156724
version: 1
top level: BBa_S04691
location: https://synbiohub.org/public/igem/BBa_S04691/annotation2156724/range2156724/1
component: BBa_J176041

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type: Range
persistent identity: range2156724
display id: range2156724
version: 1
top level: BBa_S04691
direction: direction/reverse
start: 1008
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orientation: inline

BBa_J176021
type: SequenceAnnotation
persistent identity: annotation2156725
display id: annotation2156725
version: 1
top level: BBa_S04691
location: https://synbiohub.org/public/igem/BBa_S04691/annotation2156725/range2156725/1
component: BBa_J176021

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type: Range
persistent identity: range2156725
display id: range2156725
version: 1
top level: BBa_S04691
direction: direction/reverse
start: 1014
end: 1353
orientation: inline

BBa_S04699
type: ComponentDefinition
persistent identity: BBa S04699
display id: BBa_S04699
version: 1
was generated by: iGEM to SBOL conversion
description: J176021:J176021
created: 2011-10-06T11:00:00Z
modified: 2015-05-08T01:14:44Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 10865
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Brady Laughlin
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_J176021
component: BBa_J176041
component: BBa_J176003
component: BBa_J176012
component: BBa_J176020
component: BBa_J176018
component: BBa_J176005
component: BBa_J176008
component: BBa_J176016
sequence annotation: BBa_J176021
sequence annotation: BBa_J176041
sequence annotation: BBa_J176003
sequence annotation: BBa_J176012
sequence annotation: BBa_J176020
sequence annotation: BBa_J176018
sequence annotation: BBa_J176005
sequence annotation: BBa_J176008
sequence annotation: BBa_J176016
sequence: https://synbiohub.org/public/igem/BBa_S04699_sequence/1

BBa_J176021
type: Component
persistent identity: component2156539
display id: component2156539
version: 1
top level: BBa_S04699
definition: HS4
access: public

BBa_J176021
type: Component
persistent identity: component2156541
display id: component2156541
version: 1
top level: BBa_S04699
definition: HS4
access: public

BBa_J176041
type: Component
persistent identity: component2156540
display id: component2156540
version: 1
top level: BBa_S04699
definition: scar
access: public

BBa_J176003
type: Component
persistent identity: component2156543
display id: component2156543
version: 1
top level: BBa_S04699
definition: Ubc
access: public

BBa_J176041
type: Component
persistent identity: component2156542
display id: component2156542
version: 1
top level: BBa_S04699
definition: scar
access: public

BBa_J176012
type: Component
persistent identity: component2156545
display id: component2156545
version: 1
top level: BBa_S04699
definition: K
access: public

BBa_J176041
type: Component
persistent identity: component2156544
display id: component2156544
version: 1
top level: BBa_S04699
definition: scar
access: public

BBa_J176020
type: Component
persistent identity: component2156547
display id: component2156547
version: 1
top level: BBa_S04699
definition: Gal4DB
access: public

BBa_J176041
type: Component
persistent identity: component2156546
display id: component2156546
version: 1
top level: BBa_S04699
definition: scar
access: public

BBa_J176018
type: Component
persistent identity: component2156549
display id: component2156549
version: 1
top level: BBa_S04699
definition: PEST
access: public

BBa_J176041
type: Component
persistent identity: component2156548
display id: component2156548
version: 1
top level: BBa_S04699
definition: scar
access: public

BBa_J176005
type: Component
persistent identity: component2156551
display id: component2156551
version: 1
top level: BBa_S04699
definition: mCh
access: public

BBa_J176041
type: Component
persistent identity: component2156550
display id: component2156550
version: 1
top level: BBa_S04699
definition: scar
access: public

BBa_J176005
type: Component
persistent identity: component2156553
display id: component2156553
version: 1
top level: BBa_S04699
definition: mCh
access: public

BBa_J176041
type: Component
persistent identity: component2156552
display id: component2156552
version: 1
top level: BBa_S04699
definition: scar
access: public

BBa_J176008
type: Component
persistent identity: component2156555
display id: component2156555
version: 1
top level: BBa_S04699
definition: NLS
access: public

BBa_J176041
type: Component
persistent identity: component2156554
display id: component2156554
version: 1
top level: BBa_S04699
definition: scar
access: public

BBa_J176016
type: Component
persistent identity: component2156557
display id: component2156557
version: 1
top level: BBa_S04699
definition: STOP
access: public

BBa_J176041
type: Component
persistent identity: component2156556
display id: component2156556
version: 1
top level: BBa_S04699
definition: scar
access: public

BBa_J176021
type: SequenceAnnotation
persistent identity: annotation2156539
display id: annotation2156539
version: 1
top level: BBa_S04699
location: https://synbiohub.org/public/igem/BBa_S04699/annotation2156539/range2156539/1
component: BBa_J176021

https://synbiohub.org/public/igem/BBa_S04699/annotation2156539/range2156539/1
type: Range
persistent identity: range2156539
display id: range2156539
version: 1
top level: BBa_S04699
direction: direction/reverse
start: 1
end: 340
orientation: inline

BBa_J176041
type: SequenceAnnotation
persistent identity: annotation2156540
display id: annotation2156540
version: 1
top level: BBa_S04699
location: https://synbiohub.org/public/igem/BBa_S04699/annotation2156540/range2156540/1
component: BBa_J176041

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type: Range
persistent identity: range2156540
display id: range2156540
version: 1
top level: BBa_S04699
direction: direction/reverse
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orientation: inline

BBa_J176021
type: SequenceAnnotation
persistent identity: annotation2156541
display id: annotation2156541
version: 1
top level: BBa_S04699
location: https://synbiohub.org/public/igem/BBa_S04699/annotation2156541/range2156541/1
component: BBa_J176021

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type: Range
persistent identity: range2156541
display id: range2156541
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direction: direction/reverse
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orientation: inline

BBa_J176041
type: SequenceAnnotation
persistent identity: annotation2156542
display id: annotation2156542
version: 1
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component: BBa_J176041

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type: Range
persistent identity: range2156542
display id: range2156542
version: 1
top level: BBa_S04699
direction: direction/reverse
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BBa_J176003
type: SequenceAnnotation
persistent identity: annotation2156543
display id: annotation2156543
version: 1
top level: BBa_S04699
location: https://synbiohub.org/public/igem/BBa_S04699/annotation2156543/range2156543/1
component: BBa_J176003

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persistent identity: range2156543
display id: range2156543
version: 1
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BBa_J176041
type: SequenceAnnotation
persistent identity: annotation2156544
display id: annotation2156544
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top level: BBa_S04699
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BBa_J176012
type: SequenceAnnotation
persistent identity: annotation2156545
display id: annotation2156545
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persistent identity: range2156545
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BBa_J176041
type: SequenceAnnotation
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BBa_J176020
type: SequenceAnnotation
persistent identity: annotation2156547
display id: annotation2156547
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BBa_J176041
type: SequenceAnnotation
persistent identity: annotation2156548
display id: annotation2156548
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display id: range2156548
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BBa_J176018
type: SequenceAnnotation
persistent identity: annotation2156549
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BBa_J176041
type: SequenceAnnotation
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BBa_J176005
type: SequenceAnnotation
persistent identity: annotation2156551
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top level: BBa_S04699
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BBa_J176041
type: SequenceAnnotation
persistent identity: annotation2156552
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BBa_J176005
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BBa_J176041
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top level: BBa_S04699
direction: direction/reverse
start: 3512
end: 3517
orientation: inline

BBa_J176008
type: SequenceAnnotation
persistent identity: annotation2156555
display id: annotation2156555
version: 1
top level: BBa_S04699
location: https://synbiohub.org/public/igem/BBa_S04699/annotation2156555/range2156555/1
component: BBa_J176008

https://synbiohub.org/public/igem/BBa_S04699/annotation2156555/range2156555/1
type: Range
persistent identity: range2156555
display id: range2156555
version: 1
top level: BBa_S04699
direction: direction/reverse
start: 3518
end: 3538
orientation: inline

BBa_J176041
type: SequenceAnnotation
persistent identity: annotation2156556
display id: annotation2156556
version: 1
top level: BBa_S04699
location: https://synbiohub.org/public/igem/BBa_S04699/annotation2156556/range2156556/1
component: BBa_J176041

https://synbiohub.org/public/igem/BBa_S04699/annotation2156556/range2156556/1
type: Range
persistent identity: range2156556
display id: range2156556
version: 1
top level: BBa_S04699
direction: direction/reverse
start: 3539
end: 3544
orientation: inline

BBa_J176016
type: SequenceAnnotation
persistent identity: annotation2156557
display id: annotation2156557
version: 1
top level: BBa_S04699
location: https://synbiohub.org/public/igem/BBa_S04699/annotation2156557/range2156557/1
component: BBa_J176016

https://synbiohub.org/public/igem/BBa_S04699/annotation2156557/range2156557/1
type: Range
persistent identity: range2156557
display id: range2156557
version: 1
top level: BBa_S04699
direction: direction/reverse
start: 3545
end: 3557
orientation: inline

Ubc
type: ComponentDefinition
persistent identity: BBa J176003
display id: BBa_J176003
version: 1
was generated by: iGEM to SBOL conversion
description: Ubc
created: 2011-09-28T11:00:00Z
modified: 2015-08-31T04:08:33Z
mutable provenance: TBA
owned by: james
owned by: myers
mutable description: Ubc constitutive promoter
discontinued: false
dominant: false
experience: experience/Works
group u list: _863_
m user id: 0
owner id: 10865
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: TBA
star: false
creator: Karmella Haynes
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J176003_sequence/1

mCh
type: ComponentDefinition
persistent identity: BBa J176005
display id: BBa_J176005
version: 1
was generated by: iGEM to SBOL conversion
description: mCh
created: 2011-09-28T11:00:00Z
modified: 2015-08-31T04:08:33Z
mutable provenance: TBA
owned by: james
owned by: myers
mutable description: Humanized mCherry fluorescent protein
discontinued: false
dominant: false
experience: experience/Works
group u list: _863_
m user id: 0
owner id: 10865
owning group id: 9
sample status: It's complicated
sample status: In stock
status: status/Available
status: status/Planning
bookmark: false
mutable notes: TBA
star: false
creator: Karmella Haynes
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_J176005_sequence/1

NLS
type: ComponentDefinition
persistent identity: BBa J176008
display id: BBa_J176008
version: 1
was generated by: iGEM to SBOL conversion
description: NLS
created: 2011-09-28T11:00:00Z
modified: 2015-08-31T04:08:33Z
mutable provenance: TBA
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: SV40 nuclear localization sequence
discontinued: false
dominant: false
experience: experience/Works
group u list: _863_
m user id: 0
owner id: 10865
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TBA
star: false
creator: Karmella Haynes
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence: https://synbiohub.org/public/igem/BBa_J176008_sequence/1

K
type: ComponentDefinition
persistent identity: BBa J176012
display id: BBa_J176012
version: 1
was generated by: iGEM to SBOL conversion
description: Kozak
created: 2011-09-28T11:00:00Z
modified: 2015-08-31T04:08:33Z
mutable provenance: TBA
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: yeast/ human Kozak plus start codon
discontinued: false
dominant: false
experience: experience/Works
group u list: _863_
m user id: 0
owner id: 10865
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TBA
star: false
creator: Brady Laughlin
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence: https://synbiohub.org/public/igem/BBa_J176012_sequence/1

STOP
type: ComponentDefinition
persistent identity: BBa J176016
display id: BBa_J176016
version: 1
was generated by: iGEM to SBOL conversion
description: STOP
created: 2011-09-28T11:00:00Z
modified: 2015-08-31T04:08:33Z
mutable provenance: TBA
owned by: james
owned by: myers
mutable description: contains stop codons TAA and TGA in frame
discontinued: false
dominant: false
experience: experience/Works
group u list: _863_
m user id: 0
owner id: 10865
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: TBA
star: false
creator: Karmella Haynes
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence: https://synbiohub.org/public/igem/BBa_J176016_sequence/1

PEST
type: ComponentDefinition
persistent identity: BBa J176018
display id: BBa_J176018
version: 1
was generated by: iGEM to SBOL conversion
description: PEST
created: 2011-09-28T11:00:00Z
modified: 2015-08-31T04:08:33Z
mutable provenance: TBA
owned by: james
owned by: myers
mutable description: degradation tag
discontinued: false
dominant: false
experience: experience/None
group u list: _863_
m user id: 0
owner id: 10865
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: TBA
star: false
creator: Karmella Haynes
type: DnaRegion
role: partType/Tag
role: SO:0000324
sequence: https://synbiohub.org/public/igem/BBa_J176018_sequence/1

Gal4DB
type: ComponentDefinition
persistent identity: BBa J176020
display id: BBa_J176020
version: 1
was generated by: iGEM to SBOL conversion
description: Gal4 DB
created: 2011-09-28T11:00:00Z
modified: 2015-08-31T04:08:33Z
mutable provenance: TBA
owned by: james
owned by: myers
mutable description: Gal4 DNA binding domain (remade by KAH)
discontinued: false
dominant: false
experience: experience/Works
group u list: _863_
m user id: 0
owner id: 10865
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: TBA
star: false
creator: Karmella Haynes
type: DnaRegion
role: partType/Protein Domain
role: SO:0000417
sequence: https://synbiohub.org/public/igem/BBa_J176020_sequence/1

HS4
type: ComponentDefinition
persistent identity: BBa J176021
display id: BBa_J176021
version: 1
was generated by: iGEM to SBOL conversion
description: HS4
created: 2011-09-28T11:00:00Z
modified: 2015-08-31T04:08:33Z
mutable provenance: TBA
owned by: james
owned by: myers
mutable description: chicken beta-globin gene insulator
discontinued: false
dominant: false
experience: experience/None
group u list: _863_
m user id: 0
owner id: 10865
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: TBA
star: false
creator: Karmella Haynes
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_J176021_sequence/1

miRts luc
type: ComponentDefinition
persistent identity: BBa J176025
display id: BBa_J176025
version: 1
was generated by: iGEM to SBOL conversion
description: miRts luc
created: 2011-09-28T11:00:00Z
modified: 2015-08-31T04:08:33Z
mutable provenance: TBA
owned by: james
owned by: myers
mutable description: miRNA target for luciferase RNAi
discontinued: false
dominant: false
experience: experience/Works
group u list: _863_
m user id: 0
owner id: 10865
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: TBA
star: false
creator: Karmella Haynes
type: DnaRegion
role: partType/RNA
role: SO:0000834
sequence annotation: repeat 1
sequence annotation: repeat 2
sequence annotation: repeat 3
sequence annotation: repeat 4
sequence annotation: repeat 5
sequence: https://synbiohub.org/public/igem/BBa_J176025_sequence/1

repeat 1
type: SequenceAnnotation
persistent identity: annotation2166807
display id: annotation2166807
version: 1
top level: miRts luc
location: https://synbiohub.org/public/igem/BBa_J176025/annotation2166807/range2166807/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_J176025/annotation2166807/range2166807/1
type: Range
persistent identity: range2166807
display id: range2166807
version: 1
top level: miRts luc
direction: direction/forward
start: 28
end: 46
orientation: inline

repeat 2
type: SequenceAnnotation
persistent identity: annotation2166808
display id: annotation2166808
version: 1
top level: miRts luc
location: https://synbiohub.org/public/igem/BBa_J176025/annotation2166808/range2166808/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_J176025/annotation2166808/range2166808/1
type: Range
persistent identity: range2166808
display id: range2166808
version: 1
top level: miRts luc
direction: direction/forward
start: 55
end: 73
orientation: inline

repeat 3
type: SequenceAnnotation
persistent identity: annotation2166809
display id: annotation2166809
version: 1
top level: miRts luc
location: https://synbiohub.org/public/igem/BBa_J176025/annotation2166809/range2166809/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J176025/annotation2166809/range2166809/1
type: Range
persistent identity: range2166809
display id: range2166809
version: 1
top level: miRts luc
direction: direction/forward
start: 82
end: 100
orientation: inline

repeat 4
type: SequenceAnnotation
persistent identity: annotation2166810
display id: annotation2166810
version: 1
top level: miRts luc
location: https://synbiohub.org/public/igem/BBa_J176025/annotation2166810/range2166810/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J176025/annotation2166810/range2166810/1
type: Range
persistent identity: range2166810
display id: range2166810
version: 1
top level: miRts luc
direction: direction/forward
start: 109
end: 127
orientation: inline

repeat 5
type: SequenceAnnotation
persistent identity: annotation2166811
display id: annotation2166811
version: 1
top level: miRts luc
location: https://synbiohub.org/public/igem/BBa_J176025/annotation2166811/range2166811/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J176025/annotation2166811/range2166811/1
type: Range
persistent identity: range2166811
display id: range2166811
version: 1
top level: miRts luc
direction: direction/forward
start: 135
end: 153
orientation: inline

polyA
type: ComponentDefinition
persistent identity: BBa J176042
display id: BBa_J176042
version: 1
was generated by: iGEM to SBOL conversion
description: polyA
created: 2011-10-06T11:00:00Z
modified: 2015-08-31T04:08:34Z
mutable provenance: Originally from the Silver Lab BioBrick collection. Biological source unknown. This part was created by David Drubin.
owned by: james
owned by: myers
mutable description: Polyadenylation signal for mammalian genes
discontinued: false
dominant: false
experience: experience/None
group u list: _863_
m user id: 0
owner id: 1144
owning group id: 61
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This is the first version of polyA that we used to build many of our mammalian parts, until we switched to a streamlined, shorter polyA (polyA2, BBa_J176009).
star: false
creator: Karmella Haynes
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence annotation: questionable base call
sequence: https://synbiohub.org/public/igem/BBa_J176042_sequence/1

questionable base call
type: SequenceAnnotation
persistent identity: annotation2156673
display id: annotation2156673
version: 1
top level: polyA
location: https://synbiohub.org/public/igem/BBa_J176042/annotation2156673/range2156673/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_J176042/annotation2156673/range2156673/1
type: Range
persistent identity: range2156673
display id: range2156673
version: 1
top level: polyA
direction: direction/forward
start: 128
end: 128
orientation: inline

https://synbiohub.org/public/igem/BBa_J176046_sequence/1
type: Sequence
persistent identity: BBa J176046 sequence
display id: BBa_J176046_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttaattaactcgagtcctaaagaagtcggcttctccatcggatcctgatcaggggagctcacggggacagcccccccccaaagcccccagggatgtaattacgtccctcccccgctagggggcagcagcgaccgcccggggctccgctccggtccggcgctccccccgcatcccgagccggcagcgtgcggggacagcccgggcacggggaaggtggcacgggatcgctttcctctgaacgcttctcgctgctctttgagcctgcaaacacctgggggatacggggaaaatgatcaagatcttctacactgggacgcaaggattatcctcgaggcgcgccactagattaattaactcgagtcctaaagaagtcggcttctccatcggatcctgatcaggggagctcacggggacagcccccccccaaagcccccagggatgtaattacgtccctcccccgctagggggcagcagcgaccgcccggggctccgctccggtccggcgctccccccgcatcccgagccggcagcgtgcggggacagcccgggcacggggaaggtggcacgggatcgctttcctctgaacgcttctcgctgctctttgagcctgcaaacacctgggggatacggggaaaatgatcaagatcttctacactgggacgcaaggattatcctcgaggcgcgccactagaaaactggcctccgcgccgggcttttggcgcctcccgcgggcgcccccctcctcacggcgagcgctgccacgtcagacgaagggcgcagcgagcgtcctgatccttccgcccggacgctcaggacagcggcccgctgctcataagactcggccttagaaccccagtatcagcagaaggacattttaggacgggacttgggtgactctagggcactggttttctttccagagagcggaacaggcgaggaaaagtagtcccttctcggcgattctgcggagggatctccgtggggcggtgaacgccgatgattatataaggacgcgccgggtgtggcacagctagttactagacccgccgccaccatggagactagacccgccgccaccatggagactagaatgaagctactgtcttctatcgaacaagcatgcgatatttgccgacttaaaaagctcaagtgctccaaagaaaaaccgaagtgcgccaagtgtctgaagaacaactgggagtgtcgctactctcccaaaaccaaaaggtctccgctgactagggcacatctgacagaagtggaatcaaggctagaaagactggaacagctatttctactgatttttcctcgagaagaccttgacatgattttgaaaatggattctttacaggatataaaagcattgttaacaggattatttgtacaagataatgtgaataaagatgccgtcacagatagattggcttcagtggagactgatatgcctctaacattgagacagcatagaataagtgcgacatcatcatcggaagagagtagtaacaaaggtcaaagacagttgactgtatcgccggaatttccggggatcactagagcatccaacttgaacatttcgagaaagcttaccatatcaaccccatcatgctctttcgaaaattcaaatagcacatccattccttcgcccgcttcctcatctcaaagccacactccaatgagaaacatgagctcactctctgataacagcgttttcagccggaatatggaacaatcatcaccaatcactccaagtatgtaccaatttggtcagcagcagtcaaacagtatatgtggtagcaccgttagtgtgaatagtctggtgaatacaaataacaaacaaaggatctacgaacaaatcacgggtcctaacagcaataacgcaaccaatgattatattgatttgctaaacctaaatgagtctaacaaggaaaaccaaaatcccgcaacggcgcattacctcaatgggggcccacccaagacaagcttcattaaccatggaatgt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J176041_sequence/1
type: Sequence
persistent identity: BBa J176041 sequence
display id: BBa_J176041_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: actaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S04691_sequence/1
type: Sequence
persistent identity: BBa S04691 sequence
display id: BBa_S04691_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atagttgtttaaacgagctcgctagcaaatcaagagtcctcataaaggaattaaaatcaagagtcctcataaagtctagcaaatcaagagtcctcataaagctagacaaatcaagagtcctcataaataattccaatcaagagtcctcataaagtcgacctgcaagcatgcaagctgatccggctgcactagaagacatgataagatacattgatgagtttggacaaaccacaactagaatgcagtgaaaaaaatgctttatttgtgaaatttgtgatgctattgctttatttgtaaccattataagctgcaataaacaagttaacaacaacaattgcattcattttatgtttcaggttcagggggaggtgtgggaggttttttaaagcaagtaaaacctctacaaatgtggtatggctgattatgatcctgcaagcctcgtcgtcctggccggaccacgctatctgtgcaaggtccccggccccggacgcgcgctccatgagcagagcgcccgccgccgaggcgaagactcgggcggcgccctgcccgtcccaccaggtcaacaggcggtaaccggcctcttcatcgggaatgcgcgcgaccttcagcatcgccggcatgtccccctggcggacgggaagtatccagctcgaccaagcttactagattaattaactcgagtcctaaagaagtcggcttctccatcggatcctgatcaggggagctcacggggacagcccccccccaaagcccccagggatgtaattacgtccctcccccgctagggggcagcagcgaccgcccggggctccgctccggtccggcgctccccccgcatcccgagccggcagcgtgcggggacagcccgggcacggggaaggtggcacgggatcgctttcctctgaacgcttctcgctgctctttgagcctgcaaacacctgggggatacggggaaaatgatcaagatcttctacactgggacgcaaggattatcctcgaggcgcgccactagattaattaactcgagtcctaaagaagtcggcttctccatcggatcctgatcaggggagctcacggggacagcccccccccaaagcccccagggatgtaattacgtccctcccccgctagggggcagcagcgaccgcccggggctccgctccggtccggcgctccccccgcatcccgagccggcagcgtgcggggacagcccgggcacggggaaggtggcacgggatcgctttcctctgaacgcttctcgctgctctttgagcctgcaaacacctgggggatacggggaaaatgatcaagatcttctacactgggacgcaaggattatcctcgaggcgcgcc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S04699_sequence/1
type: Sequence
persistent identity: BBa S04699 sequence
display id: BBa_S04699_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttaattaactcgagtcctaaagaagtcggcttctccatcggatcctgatcaggggagctcacggggacagcccccccccaaagcccccagggatgtaattacgtccctcccccgctagggggcagcagcgaccgcccggggctccgctccggtccggcgctccccccgcatcccgagccggcagcgtgcggggacagcccgggcacggggaaggtggcacgggatcgctttcctctgaacgcttctcgctgctctttgagcctgcaaacacctgggggatacggggaaaatgatcaagatcttctacactgggacgcaaggattatcctcgaggcgcgccactagattaattaactcgagtcctaaagaagtcggcttctccatcggatcctgatcaggggagctcacggggacagcccccccccaaagcccccagggatgtaattacgtccctcccccgctagggggcagcagcgaccgcccggggctccgctccggtccggcgctccccccgcatcccgagccggcagcgtgcggggacagcccgggcacggggaaggtggcacgggatcgctttcctctgaacgcttctcgctgctctttgagcctgcaaacacctgggggatacggggaaaatgatcaagatcttctacactgggacgcaaggattatcctcgaggcgcgccactagaaaactggcctccgcgccgggcttttggcgcctcccgcgggcgcccccctcctcacggcgagcgctgccacgtcagacgaagggcgcagcgagcgtcctgatccttccgcccggacgctcaggacagcggcccgctgctcataagactcggccttagaaccccagtatcagcagaaggacattttaggacgggacttgggtgactctagggcactggttttctttccagagagcggaacaggcgaggaaaagtagtcccttctcggcgattctgcggagggatctccgtggggcggtgaacgccgatgattatataaggacgcgccgggtgtggcacagctagttactagacccgccgccaccatggagactagacccgccgccaccatggagactagaatgaagctactgtcttctatcgaacaagcatgcgatatttgccgacttaaaaagctcaagtgctccaaagaaaaaccgaagtgcgccaagtgtctgaagaacaactgggagtgtcgctactctcccaaaaccaaaaggtctccgctgactagggcacatctgacagaagtggaatcaaggctagaaagactggaacagctatttctactgatttttcctcgagaagaccttgacatgattttgaaaatggattctttacaggatataaaagcattgttaacaggattatttgtacaagataatgtgaataaagatgccgtcacagatagattggcttcagtggagactgatatgcctctaacattgagacagcatagaataagtgcgacatcatcatcggaagagagtagtaacaaaggtcaaagacagttgactgtatcgccggaatttccggggatcactagagcatccaacttgaacatttcgagaaagcttaccatatcaaccccatcatgctctttcgaaaattcaaatagcacatccattccttcgcccgcttcctcatctcaaagccacactccaatgagaaacatgagctcactctctgataacagcgttttcagccggaatatggaacaatcatcaccaatcactccaagtatgtaccaatttggtcagcagcagtcaaacagtatatgtggtagcaccgttagtgtgaatagtctggtgaatacaaataacaaacaaaggatctacgaacaaatcacgggtcctaacagcaataacgcaaccaatgattatattgatttgctaaacctaaatgagtctaacaaggaaaaccaaaatcccgcaacggcgcattacctcaatgggggcccacccaagacaagcttcattaaccatggaatgt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J176003_sequence/1
type: Sequence
persistent identity: BBa J176003 sequence
display id: BBa_J176003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaactggcctccgcgccgggcttttggcgcctcccgcgggcgcccccctcctcacggcgagcgctgccacgtcagacgaagggcgcagcgagcgtcctgatccttccgcccggacgctcaggacagcggcccgctgctcataagactcggccttagaaccccagtatcagcagaaggacattttaggacgggacttgggtgactctagggcactggttttctttccagagagcggaacaggcgaggaaaagtagtcccttctcggcgattctgcggagggatctccgtggggcggtgaacgccgatgattatataaggacgcgccgggtgtggcacagctagttactagacccgccgccaccatggag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J176005_sequence/1
type: Sequence
persistent identity: BBa J176005 sequence
display id: BBa_J176005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctacccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J176008_sequence/1
type: Sequence
persistent identity: BBa J176008 sequence
display id: BBa_J176008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cccaagaaaaagcgcaaggta
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J176012_sequence/1
type: Sequence
persistent identity: BBa J176012 sequence
display id: BBa_J176012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cccgccgccaccatggag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J176016_sequence/1
type: Sequence
persistent identity: BBa J176016 sequence
display id: BBa_J176016_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcgtaaagctgag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J176018_sequence/1
type: Sequence
persistent identity: BBa J176018 sequence
display id: BBa_J176018_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcatccaacttgaacatttcgagaaagcttaccatatcaaccccatcatgctctttcgaaaattcaaatagcacatccattccttcgcccgcttcctcatctcaaagccacactccaatgagaaacatgagctcactctctgataacagcgttttcagccggaatatggaacaatcatcaccaatcactccaagtatgtaccaatttggtcagcagcagtcaaacagtatatgtggtagcaccgttagtgtgaatagtctggtgaatacaaataacaaacaaaggatctacgaacaaatcacgggtcctaacagcaataacgcaaccaatgattatattgatttgctaaacctaaatgagtctaacaaggaaaaccaaaatcccgcaacggcgcattacctcaatgggggcccacccaagacaagcttcattaaccatggaatgttcccctcgccaactgggaccataaatagcggtaaatctagcagtgcctcatctttaatttcttttggtatgggcaatacccaagtaata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J176020_sequence/1
type: Sequence
persistent identity: BBa J176020 sequence
display id: BBa_J176020_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaagctactgtcttctatcgaacaagcatgcgatatttgccgacttaaaaagctcaagtgctccaaagaaaaaccgaagtgcgccaagtgtctgaagaacaactgggagtgtcgctactctcccaaaaccaaaaggtctccgctgactagggcacatctgacagaagtggaatcaaggctagaaagactggaacagctatttctactgatttttcctcgagaagaccttgacatgattttgaaaatggattctttacaggatataaaagcattgttaacaggattatttgtacaagataatgtgaataaagatgccgtcacagatagattggcttcagtggagactgatatgcctctaacattgagacagcatagaataagtgcgacatcatcatcggaagagagtagtaacaaaggtcaaagacagttgactgtatcgccggaatttccggggatc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J176021_sequence/1
type: Sequence
persistent identity: BBa J176021 sequence
display id: BBa_J176021_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttaattaactcgagtcctaaagaagtcggcttctccatcggatcctgatcaggggagctcacggggacagcccccccccaaagcccccagggatgtaattacgtccctcccccgctagggggcagcagcgaccgcccggggctccgctccggtccggcgctccccccgcatcccgagccggcagcgtgcggggacagcccgggcacggggaaggtggcacgggatcgctttcctctgaacgcttctcgctgctctttgagcctgcaaacacctgggggatacggggaaaatgatcaagatcttctacactgggacgcaaggattatcctcgaggcgcgcc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J176025_sequence/1
type: Sequence
persistent identity: BBa J176025 sequence
display id: BBa_J176025_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atagttgtttaaacgagctcgctagcaaatcaagagtcctcataaaggaattaaaatcaagagtcctcataaagtctagcaaatcaagagtcctcataaagctagacaaatcaagagtcctcataaataattccaatcaagagtcctcataaagtcgacctgcaagcatgcaagctgatccggctgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J176042_sequence/1
type: Sequence
persistent identity: BBa J176042 sequence
display id: BBa_J176042_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agacatgataagatacattgatgagtttggacaaaccacaactagaatgcagtgaaaaaaatgctttatttgtgaaatttgtgatgctattgctttatttgtaaccattataagctgcaataaacaagttaacaacaacaattgcattcattttatgtttcaggttcagggggaggtgtgggaggttttttaaagcaagtaaaacctctacaaatgtggtatggctgattatgatcctgcaagcctcgtcgtcctggccggaccacgctatctgtgcaaggtccccggccccggacgcgcgctccatgagcagagcgcccgccgccgaggcgaagactcgggcggcgccctgcccgtcccaccaggtcaacaggcggtaaccggcctcttcatcgggaatgcgcgcgaccttcagcatcgccggcatgtccccctggcggacgggaagtatccagctcgaccaagctt
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z