LOCUS BBa_J176047 5064 bp DNA linear 22-Dec-2024 DEFINITION Luc miRNA RFP-reporter 2 ACCESSION BBa_J176047 VERSION BBa_J176047.1 FEATURES Location/Qualifiers misc_feature 1..340 /label=BBa_J176021 misc_feature 341..346 /label=BBa_J176041 misc_feature 347..686 /label=BBa_J176021 misc_feature 687..692 /label=BBa_J176041 misc_feature 945..945 /label=mutated SpeI misc_feature 1203..1203 /label=mutated PstI promoter 693..1208 /label=BBa_J176028 misc_feature 1209..1214 /label=BBa_J176041 RBS 1215..1232 /label=BBa_J176012 misc_feature 1233..1238 /label=BBa_J176041 misc_feature 1239..1697 /label=BBa_J176020 misc_feature 1698..1703 /label=BBa_J176041 misc_feature 1704..2237 /label=BBa_J176018 misc_feature 2238..2243 /label=BBa_J176041 misc_feature 2244..2948 /label=BBa_J176005 misc_feature 2949..2954 /label=BBa_J176041 misc_feature 2955..3659 /label=BBa_J176005 misc_feature 3660..3665 /label=BBa_J176041 misc_feature 3666..3686 /label=BBa_J176008 misc_feature 3687..3692 /label=BBa_J176041 terminator 3693..3705 /label=BBa_J176016 misc_feature 1..3705 /label=BBa_S04700 misc_feature 3706..3711 /label=BBa_J176041 misc_feature 3739..3757 /label=repeat 1 misc_feature 3766..3784 /label=repeat 2 misc_feature 3793..3811 /label=repeat 3 misc_feature 3820..3838 /label=repeat 4 misc_feature 3846..3864 /label=repeat 5 misc_feature 3712..3898 /label=BBa_J176025 misc_feature 3899..3904 /label=BBa_J176041 misc_feature 4032..4032 /label=questionable base call misc_feature 3905..4372 /label=BBa_J176042 misc_feature 4373..4378 /label=BBa_J176041 misc_feature 4379..4718 /label=BBa_J176021 misc_feature 4719..4724 /label=BBa_J176041 misc_feature 4725..5064 /label=BBa_J176021 misc_feature 3712..5064 /label=BBa_S04691 ORIGIN 1 ttaattaact cgagtcctaa agaagtcggc ttctccatcg gatcctgatc aggggagctc 61 acggggacag ccccccccca aagcccccag ggatgtaatt acgtccctcc cccgctaggg 121 ggcagcagcg accgcccggg gctccgctcc ggtccggcgc tccccccgca tcccgagccg 181 gcagcgtgcg gggacagccc gggcacgggg aaggtggcac gggatcgctt tcctctgaac 241 gcttctcgct gctctttgag cctgcaaaca cctgggggat acggggaaaa tgatcaagat 301 cttctacact gggacgcaag gattatcctc gaggcgcgcc actagattaa ttaactcgag 361 tcctaaagaa gtcggcttct ccatcggatc ctgatcaggg gagctcacgg ggacagcccc 421 cccccaaagc ccccagggat gtaattacgt ccctcccccg ctagggggca gcagcgaccg 481 cccggggctc cgctccggtc cggcgctccc cccgcatccc gagccggcag cgtgcgggga 541 cagcccgggc acggggaagg tggcacggga tcgctttcct ctgaacgctt ctcgctgctc 601 tttgagcctg caaacacctg ggggatacgg ggaaaatgat caagatcttc tacactggga 661 cgcaaggatt atcctcgagg cgcgccacta gactaccggg taggggaggc gcttttccca 721 aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta cacaagtggc 781 ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc gttctttggt 841 ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc cgccccgcag 901 ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcacaagtct cgtgcagatg 961 gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca atagcagctt 1021 tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg cgggctcagg 1081 ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc attctgcacg 1141 cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg cctttcgacc 1201 tggagcccac tagacccgcc gccaccatgg agactagaat gaagctactg tcttctatcg 1261 aacaagcatg cgatatttgc cgacttaaaa agctcaagtg ctccaaagaa aaaccgaagt 1321 gcgccaagtg tctgaagaac aactgggagt gtcgctactc tcccaaaacc aaaaggtctc 1381 cgctgactag ggcacatctg acagaagtgg aatcaaggct agaaagactg gaacagctat 1441 ttctactgat ttttcctcga gaagaccttg acatgatttt gaaaatggat tctttacagg 1501 atataaaagc attgttaaca ggattatttg tacaagataa tgtgaataaa gatgccgtca 1561 cagatagatt ggcttcagtg gagactgata tgcctctaac attgagacag catagaataa 1621 gtgcgacatc atcatcggaa gagagtagta acaaaggtca aagacagttg actgtatcgc 1681 cggaatttcc ggggatcact agagcatcca acttgaacat ttcgagaaag cttaccatat 1741 caaccccatc atgctctttc gaaaattcaa atagcacatc cattccttcg cccgcttcct 1801 catctcaaag ccacactcca atgagaaaca tgagctcact ctctgataac agcgttttca 1861 gccggaatat ggaacaatca tcaccaatca ctccaagtat gtaccaattt ggtcagcagc 1921 agtcaaacag tatatgtggt agcaccgtta gtgtgaatag tctggtgaat acaaataaca 1981 aacaaaggat ctacgaacaa atcacgggtc ctaacagcaa taacgcaacc aatgattata 2041 ttgatttgct aaacctaaat gagtctaaca aggaaaacca aaatcccgca acggcgcatt 2101 acctcaatgg gggcccaccc aagacaagct tcattaacca tggaatgttc ccctcgccaa 2161 ctgggaccat aaatagcggt aaatctagca gtgcctcatc tttaatttct tttggtatgg 2221 gcaataccca agtaataact agagtgagca agggcgagga ggataacatg gccatcatca 2281 aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga 2341 tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga 2401 ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc atgtacggct 2461 ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttgaagctg tccttccccg 2521 agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc 2581 aggactcctc cttgcaggac ggcgagttca tctacaaggt gaagctgcgc ggcaccaact 2641 tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc tcctccgagc 2701 ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa gcagaggctg aagctgaagg 2761 acggcggcca ctacgacgct gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc 2821 tacccggcgc ctacaacgtc aacatcaagt tggacatcac ctcccacaac gaggactaca 2881 ccatcgtgga acagtacgaa cgcgccgagg gccgccactc caccggcggc atggacgagc 2941 tgtacaagac tagagtgagc aagggcgagg aggataacat ggccatcatc aaggagttca 3001 tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg 3061 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg 3121 gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct 3181 acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca 3241 agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct 3301 ccttgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg 3361 acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc 3421 ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc 3481 actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctacccggcg 3541 cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg 3601 aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaaga 3661 ctagacccaa gaaaaagcgc aaggtaacta gagcgtaaag ctgagactag aatagttgtt 3721 taaacgagct cgctagcaaa tcaagagtcc tcataaagga attaaaatca agagtcctca 3781 taaagtctag caaatcaaga gtcctcataa agctagacaa atcaagagtc ctcataaata 3841 attccaatca agagtcctca taaagtcgac ctgcaagcat gcaagctgat ccggctgcac 3901 tagaagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa 3961 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct 4021 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg 4081 tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc 4141 ctgcaagcct cgtcgtcctg gccggaccac gctatctgtg caaggtcccc ggccccggac 4201 gcgcgctcca tgagcagagc gcccgccgcc gaggcgaaga ctcgggcggc gccctgcccg 4261 tcccaccagg tcaacaggcg gtaaccggcc tcttcatcgg gaatgcgcgc gaccttcagc 4321 atcgccggca tgtccccctg gcggacggga agtatccagc tcgaccaagc ttactagatt 4381 aattaactcg agtcctaaag aagtcggctt ctccatcgga tcctgatcag gggagctcac 4441 ggggacagcc cccccccaaa gcccccaggg atgtaattac gtccctcccc cgctaggggg 4501 cagcagcgac cgcccggggc tccgctccgg tccggcgctc cccccgcatc ccgagccggc 4561 agcgtgcggg gacagcccgg gcacggggaa ggtggcacgg gatcgctttc ctctgaacgc 4621 ttctcgctgc tctttgagcc tgcaaacacc tgggggatac ggggaaaatg atcaagatct 4681 tctacactgg gacgcaagga ttatcctcga ggcgcgccac tagattaatt aactcgagtc 4741 ctaaagaagt cggcttctcc atcggatcct gatcagggga gctcacgggg acagcccccc 4801 cccaaagccc ccagggatgt aattacgtcc ctcccccgct agggggcagc agcgaccgcc 4861 cggggctccg ctccggtccg gcgctccccc cgcatcccga gccggcagcg tgcggggaca 4921 gcccgggcac ggggaaggtg gcacgggatc gctttcctct gaacgcttct cgctgctctt 4981 tgagcctgca aacacctggg ggatacgggg aaaatgatca agatcttcta cactgggacg 5041 caaggattat cctcgaggcg cgcc //